ID   MED10_DICDI             Reviewed;         219 AA.
AC   Q54GE5;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 10;
DE   AltName: Full=Putative mediator complex subunit 10;
GN   Name=med10; ORFNames=DDB_G0290209;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000161; EAL62326.1; -; Genomic_DNA.
DR   RefSeq; XP_635833.1; XM_630741.1.
DR   AlphaFoldDB; Q54GE5; -.
DR   SMR; Q54GE5; -.
DR   STRING; 44689.DDB0266869; -.
DR   PaxDb; Q54GE5; -.
DR   EnsemblProtists; EAL62326; EAL62326; DDB_G0290209.
DR   GeneID; 8627541; -.
DR   KEGG; ddi:DDB_G0290209; -.
DR   dictyBase; DDB_G0290209; med10.
DR   eggNOG; KOG3046; Eukaryota.
DR   HOGENOM; CLU_1263554_0_0_1; -.
DR   InParanoid; Q54GE5; -.
DR   OMA; YSKHCME; -.
DR   PRO; PR:Q54GE5; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR   GO; GO:0070847; C:core mediator complex; ISS:dictyBase.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:dictyBase.
DR   InterPro; IPR019145; Mediator_Med10.
DR   Pfam; PF09748; Med10; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..219
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 10"
FT                   /id="PRO_0000388650"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          177..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          13..74
FT                   /evidence="ECO:0000255"
FT   COILED          166..219
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        189..219
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   219 AA;  26106 MW;  5C31AFAAE4B5BD69 CRC64;
     MEQQQPQQQN DGQQQQQQQQ QHQQQQQQQQ QQQQQQQQSE QERIYNKKQE HLPLLQTVEE
     LVEELRRSLI VVEEFQTPSQ QLLFDKLNKI INLYEKIESN RHIVNDVEIP LEIFRVIDQS
     KNPDLYVKET LQNCLSANEK TKGKIESIKN FKKELESHIS ESFASEYAEY KLLTQKKETQ
     EQQNDDQIKV DDSNNNNNNN NNNNYNNNNN NNNNNINNN