MED10_HUMAN
ID MED10_HUMAN Reviewed; 135 AA.
AC Q9BTT4; C6G491;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE AltName: Full=Mediator complex subunit 10;
DE AltName: Full=Transformation-related gene 17 protein;
DE Short=TRG-17;
DE AltName: Full=Transformation-related gene 20 protein;
DE Short=TRG-20;
GN Name=MED10; ORFNames=L6, TRG17, TRG20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15334068; DOI=10.1038/sj.onc.1207921;
RA Petroziello J., Yamane A., Westendorf L., Thompson M., McDonagh C.,
RA Cerveny C., Law C.-L., Wahl A., Carter P.;
RT "Suppression subtractive hybridization and expression profiling identifies
RT a unique set of genes overexpressed in non-small-cell lung cancer.";
RL Oncogene 23:7734-7745(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kim J.W.;
RT "Identification of a human transforming gene.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RG NHLBI resequencing and genotyping service (RS&G);
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE SMCC
RP COMPLEX.
RX PubMed=10024883; DOI=10.1016/s1097-2765(00)80178-1;
RA Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E.,
RA Qin J., Roeder R.G.;
RT "A novel human SRB/MED-containing cofactor complex, SMCC, involved in
RT transcription regulation.";
RL Mol. Cell 3:97-108(1999).
RN [7]
RP ERRATUM OF PUBMED:10024883.
RA Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E.,
RA Qin J., Roeder R.G.;
RL Mol. Cell 3:541-541(1999).
RN [8]
RP INTERACTION WITH MED6.
RX PubMed=10993082; DOI=10.1038/35024111;
RA Akoulitchev S., Chuikov S., Reinberg D.;
RT "TFIIH is negatively regulated by cdk8-containing mediator complexes.";
RL Nature 407:102-106(2000).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=15175163; DOI=10.1016/j.molcel.2004.05.006;
RA Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B.,
RA Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W.,
RA Conaway R.C.;
RT "A set of consensus mammalian mediator subunits identified by
RT multidimensional protein identification technology.";
RL Mol. Cell 14:685-691(2004).
RN [10]
RP INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED14; MED15; MED16;
RP MED17; MED18; MED19; MED20; MED23; MED24 AND MED26, IDENTIFICATION BY MASS
RP SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, AND ASSOCIATION OF
RP THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
RX PubMed=15989967; DOI=10.1016/j.molcel.2005.05.015;
RA Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T.,
RA Roeder R.G.;
RT "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation
RT enriched in RNA polymerase II and is required for ER-mediated
RT transcription.";
RL Mol. Cell 19:89-100(2005).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
CC {ECO:0000269|PubMed:10024883, ECO:0000269|PubMed:15175163,
CC ECO:0000269|PubMed:15989967}.
CC -!- INTERACTION:
CC Q9BTT4; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-394354, EBI-11096309;
CC Q9BTT4; Q99819: ARHGDIG; NbExp=3; IntAct=EBI-394354, EBI-10295284;
CC Q9BTT4; P04066: FUCA1; NbExp=3; IntAct=EBI-394354, EBI-2512153;
CC Q9BTT4; O43513: MED7; NbExp=7; IntAct=EBI-394354, EBI-394632;
CC Q9BTT4; Q9H8W5-2: TRIM45; NbExp=3; IntAct=EBI-394354, EBI-11993364;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC {ECO:0000305}.
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DR EMBL; AY598325; AAT06736.1; -; mRNA.
DR EMBL; AY277599; AAQ18038.1; -; mRNA.
DR EMBL; AY453397; AAS47514.1; -; mRNA.
DR EMBL; FJ515848; ACS13739.1; -; Genomic_DNA.
DR EMBL; CH471102; EAX08110.1; -; Genomic_DNA.
DR EMBL; BC003353; AAH03353.1; -; mRNA.
DR CCDS; CCDS34134.1; -.
DR RefSeq; NP_115662.2; NM_032286.2.
DR PDB; 7EMF; EM; 3.50 A; J=1-135.
DR PDB; 7ENA; EM; 4.07 A; j=1-135.
DR PDB; 7ENC; EM; 4.13 A; j=1-135.
DR PDB; 7ENJ; EM; 4.40 A; J=1-135.
DR PDB; 7LBM; EM; 4.80 A; v=1-135.
DR PDB; 7NVR; EM; 4.50 A; k=1-135.
DR PDBsum; 7EMF; -.
DR PDBsum; 7ENA; -.
DR PDBsum; 7ENC; -.
DR PDBsum; 7ENJ; -.
DR PDBsum; 7LBM; -.
DR PDBsum; 7NVR; -.
DR AlphaFoldDB; Q9BTT4; -.
DR SMR; Q9BTT4; -.
DR BioGRID; 123974; 100.
DR ComplexPortal; CPX-3227; Core mediator complex.
DR CORUM; Q9BTT4; -.
DR DIP; DIP-31457N; -.
DR IntAct; Q9BTT4; 84.
DR MINT; Q9BTT4; -.
DR STRING; 9606.ENSP00000255764; -.
DR iPTMnet; Q9BTT4; -.
DR PhosphoSitePlus; Q9BTT4; -.
DR BioMuta; MED10; -.
DR DMDM; 74733145; -.
DR EPD; Q9BTT4; -.
DR jPOST; Q9BTT4; -.
DR MassIVE; Q9BTT4; -.
DR MaxQB; Q9BTT4; -.
DR PaxDb; Q9BTT4; -.
DR PeptideAtlas; Q9BTT4; -.
DR PRIDE; Q9BTT4; -.
DR ProteomicsDB; 79008; -.
DR TopDownProteomics; Q9BTT4; -.
DR Antibodypedia; 43141; 39 antibodies from 19 providers.
DR DNASU; 84246; -.
DR Ensembl; ENST00000255764.4; ENSP00000255764.3; ENSG00000133398.4.
DR GeneID; 84246; -.
DR KEGG; hsa:84246; -.
DR MANE-Select; ENST00000255764.4; ENSP00000255764.3; NM_032286.3; NP_115662.2.
DR UCSC; uc003jdo.4; human.
DR CTD; 84246; -.
DR GeneCards; MED10; -.
DR HGNC; HGNC:28760; MED10.
DR HPA; ENSG00000133398; Low tissue specificity.
DR MIM; 612382; gene.
DR neXtProt; NX_Q9BTT4; -.
DR OpenTargets; ENSG00000133398; -.
DR PharmGKB; PA144596412; -.
DR VEuPathDB; HostDB:ENSG00000133398; -.
DR eggNOG; KOG3046; Eukaryota.
DR GeneTree; ENSGT00390000014501; -.
DR HOGENOM; CLU_096169_3_0_1; -.
DR InParanoid; Q9BTT4; -.
DR OMA; QYQRAKM; -.
DR OrthoDB; 1403581at2759; -.
DR PhylomeDB; Q9BTT4; -.
DR TreeFam; TF315096; -.
DR PathwayCommons; Q9BTT4; -.
DR Reactome; R-HSA-1989781; PPARA activates gene expression.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR Reactome; R-HSA-381340; Transcriptional regulation of white adipocyte differentiation.
DR SignaLink; Q9BTT4; -.
DR SIGNOR; Q9BTT4; -.
DR BioGRID-ORCS; 84246; 482 hits in 1102 CRISPR screens.
DR ChiTaRS; MED10; human.
DR GenomeRNAi; 84246; -.
DR Pharos; Q9BTT4; Tdark.
DR PRO; PR:Q9BTT4; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q9BTT4; protein.
DR Bgee; ENSG00000133398; Expressed in monocyte and 170 other tissues.
DR Genevisible; Q9BTT4; HS.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0070847; C:core mediator complex; IPI:ComplexPortal.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:ComplexPortal.
DR GO; GO:0000151; C:ubiquitin ligase complex; IEA:Ensembl.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR InterPro; IPR019145; Mediator_Med10.
DR Pfam; PF09748; Med10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..135
FT /note="Mediator of RNA polymerase II transcription subunit
FT 10"
FT /id="PRO_0000303151"
FT HELIX 8..28
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 36..59
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 65..72
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 78..116
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 118..127
FT /evidence="ECO:0007829|PDB:7EMF"
SQ SEQUENCE 135 AA; 15688 MW; F804CD76770149E1 CRC64;
MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD IDKCRQQLHD
ITVPLEVFEY IDQGRNPQLY TKECLERALA KNEQVKGKID TMKKFKSLLI QELSKVFPED
MAKYRSIRGE DHPPS
