ID   MED10_KLULA             Reviewed;         161 AA.
AC   Q6CVN3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE   AltName: Full=Mediator complex subunit 10;
GN   Name=NUT2; Synonyms=MED10; OrderedLocusNames=KLLA0B10714g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382122; CAH02399.1; -; Genomic_DNA.
DR   RefSeq; XP_452006.1; XM_452006.1.
DR   AlphaFoldDB; Q6CVN3; -.
DR   SMR; Q6CVN3; -.
DR   STRING; 28985.XP_452006.1; -.
DR   EnsemblFungi; CAH02399; CAH02399; KLLA0_B10714g.
DR   GeneID; 2897456; -.
DR   KEGG; kla:KLLA0_B10714g; -.
DR   eggNOG; KOG3046; Eukaryota.
DR   HOGENOM; CLU_096169_1_0_1; -.
DR   InParanoid; Q6CVN3; -.
DR   OMA; QYQRAKM; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR019145; Mediator_Med10.
DR   Pfam; PF09748; Med10; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..161
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   10"
FT                   /id="PRO_0000303172"
SQ   SEQUENCE   161 AA;  18583 MW;  37E79BA45AEBC72C CRC64;
     MVEQNLEKLQ TDLVETEQRL AAIIDSFIEL GVYVYDFPGT EDSMQGMVTN MQRNVDRMKQ
     LNQQANDPES QLQNFLVPLD VLQYIEDGRN PDVYTREFVE AIRRSNQYQR AKIHGLNKLR
     DSLAEKIIDE LPELQPHVES IIARTSPQRT DPISPSIVDK P