ID   MED10_NEUCR             Reviewed;         253 AA.
AC   Q7RXK7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE   AltName: Full=Mediator complex subunit 10;
GN   Name=nut2; Synonyms=med10; ORFNames=NCU00220;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC       {ECO:0000305}.
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DR   EMBL; CM002238; EAA27369.1; -; Genomic_DNA.
DR   RefSeq; XP_956605.1; XM_951512.2.
DR   AlphaFoldDB; Q7RXK7; -.
DR   SMR; Q7RXK7; -.
DR   STRING; 5141.EFNCRP00000000249; -.
DR   EnsemblFungi; EAA27369; EAA27369; NCU00220.
DR   GeneID; 3872752; -.
DR   KEGG; ncr:NCU00220; -.
DR   VEuPathDB; FungiDB:NCU00220; -.
DR   HOGENOM; CLU_096169_0_0_1; -.
DR   InParanoid; Q7RXK7; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019145; Mediator_Med10.
DR   Pfam; PF09748; Med10; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..253
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   10"
FT                   /id="PRO_0000303174"
FT   REGION          32..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          88..109
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          206..253
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..50
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        233..253
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   253 AA;  26752 MW;  4599FAD23BBF435C CRC64;
     MAPVTATHQE AQESVKNVIQ DLLNLMVQVS QYDTNPSSSN NNTPTSSRAS GGGGGGGGGH
     ASSRDIIAQS LQTLDASLLS VYRTANLLPS SPSSGPSNNQ PQQGTTELER AEAAQFASTF
     NNTHNPYAPH VHQPGPQNPG IPIPLITYVE NGRNPDVYTR EFIELVRRSN QLMRGKMHAF
     RDFRDVLAGE MEAALPELRE DIKRVVEATG GPGPAEGSEE RAREGVVGSL SAGGEGQQGQ
     GQGQQGQGQQ SGQ