MED10_SCHPO
ID MED10_SCHPO Reviewed; 144 AA.
AC P87310;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE AltName: Full=Mediator complex subunit 10;
GN Name=med10; Synonyms=nut2; ORFNames=SPBC31F10.09c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RC STRAIN=972 / ATCC 24843;
RX PubMed=10625684; DOI=10.1074/jbc.275.2.1351;
RA Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A.,
RA Gustafsson C.M.;
RT "Purification and characterization of RNA polymerase II holoenzyme from
RT Schizosaccharomyces pombe.";
RL J. Biol. Chem. 275:1351-1356(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:10625684, ECO:0000269|PubMed:11572939}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}. Nucleus envelope
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAB10086.2; -; Genomic_DNA.
DR PIR; T40212; T40212.
DR RefSeq; NP_596571.2; NM_001022492.2.
DR PDB; 5N9J; X-ray; 3.40 A; B=1-144.
DR PDBsum; 5N9J; -.
DR AlphaFoldDB; P87310; -.
DR SMR; P87310; -.
DR BioGRID; 276803; 7.
DR IntAct; P87310; 27.
DR STRING; 4896.SPBC31F10.09c.1; -.
DR iPTMnet; P87310; -.
DR MaxQB; P87310; -.
DR PaxDb; P87310; -.
DR EnsemblFungi; SPBC31F10.09c.1; SPBC31F10.09c.1:pep; SPBC31F10.09c.
DR GeneID; 2540272; -.
DR KEGG; spo:SPBC31F10.09c; -.
DR PomBase; SPBC31F10.09c; -.
DR VEuPathDB; FungiDB:SPBC31F10.09c; -.
DR eggNOG; KOG3046; Eukaryota.
DR HOGENOM; CLU_096169_1_1_1; -.
DR InParanoid; P87310; -.
DR OMA; QYQRAKM; -.
DR PRO; PR:P87310; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005635; C:nuclear envelope; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; TAS:PomBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR InterPro; IPR019145; Mediator_Med10.
DR Pfam; PF09748; Med10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..144
FT /note="Mediator of RNA polymerase II transcription subunit
FT 10"
FT /id="PRO_0000096356"
FT HELIX 9..33
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 43..65
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 71..78
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 83..122
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 127..137
FT /evidence="ECO:0007829|PDB:5N9J"
SQ SEQUENCE 144 AA; 16338 MW; A780C526591714C3 CRC64;
MLPQDDMTDE MKSLASRLED TTQAFYDLAL IVYNLEDTTP SDAIPESLDT LIRDLKSLPD
ISRKVNNLIP QDVLEYIEQG RNPDVYARQF SELVQKDNQY VNGKLYAIEG FQKAFAEEIK
QAYPEVSSVV DKILNEGKVE STVS
