MED11_HUMAN
ID MED11_HUMAN Reviewed; 117 AA.
AC Q9P086; Q6NS89;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 11;
DE AltName: Full=Mediator complex subunit 11;
GN Name=MED11; ORFNames=HSPC296;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RA Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L.,
RA Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.;
RT "Human partial CDS from CD34+ stem cells.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH MED10 AND MED22.
RX PubMed=12584197; DOI=10.1074/jbc.c300054200;
RA Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J.,
RA Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C.,
RA Conaway J.W.;
RT "Identification of mammalian Mediator subunits with similarities to yeast
RT Mediator subunits Srb5, Srb6, Med11, and Rox3.";
RL J. Biol. Chem. 278:15123-15127(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=15175163; DOI=10.1016/j.molcel.2004.05.006;
RA Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B.,
RA Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W.,
RA Conaway R.C.;
RT "A set of consensus mammalian mediator subunits identified by
RT multidimensional protein identification technology.";
RL Mol. Cell 14:685-691(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
RX PubMed=15989967; DOI=10.1016/j.molcel.2005.05.015;
RA Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T.,
RA Roeder R.G.;
RT "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation
RT enriched in RNA polymerase II and is required for ER-mediated
RT transcription.";
RL Mol. Cell 19:89-100(2005).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
CC {ECO:0000269|PubMed:15175163, ECO:0000269|PubMed:15989967}.
CC -!- INTERACTION:
CC Q9P086; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-394704, EBI-11096309;
CC Q9P086; Q96HB5: CCDC120; NbExp=3; IntAct=EBI-394704, EBI-744556;
CC Q9P086; P23434: GCSH; NbExp=3; IntAct=EBI-394704, EBI-715444;
CC Q9P086; Q15528: MED22; NbExp=3; IntAct=EBI-394704, EBI-394687;
CC Q9P086; Q9GZM8: NDEL1; NbExp=3; IntAct=EBI-394704, EBI-928842;
CC Q9P086; Q9Y5B8: NME7; NbExp=3; IntAct=EBI-394704, EBI-744782;
CC Q9P086; Q96ES7: SGF29; NbExp=3; IntAct=EBI-394704, EBI-743117;
CC Q9P086; A1L4H1: SSC5D; NbExp=3; IntAct=EBI-394704, EBI-10172867;
CC Q9P086; Q6PIF2: SYCE2; NbExp=3; IntAct=EBI-394704, EBI-11958386;
CC Q9P086; Q8N6Y0: USHBP1; NbExp=3; IntAct=EBI-394704, EBI-739895;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 11 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF28974.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF161414; AAF28974.1; ALT_INIT; mRNA.
DR EMBL; CH471108; EAW90417.1; -; Genomic_DNA.
DR EMBL; BC070377; AAH70377.1; -; mRNA.
DR CCDS; CCDS32533.1; -.
DR RefSeq; NP_001001683.1; NM_001001683.3.
DR RefSeq; NP_001291929.1; NM_001305000.1.
DR PDB; 7EMF; EM; 3.50 A; K=1-117.
DR PDB; 7ENA; EM; 4.07 A; k=1-117.
DR PDB; 7ENC; EM; 4.13 A; k=1-117.
DR PDB; 7ENJ; EM; 4.40 A; K=1-117.
DR PDB; 7LBM; EM; 4.80 A; i=1-117.
DR PDB; 7NVR; EM; 4.50 A; c=1-117.
DR PDBsum; 7EMF; -.
DR PDBsum; 7ENA; -.
DR PDBsum; 7ENC; -.
DR PDBsum; 7ENJ; -.
DR PDBsum; 7LBM; -.
DR PDBsum; 7NVR; -.
DR AlphaFoldDB; Q9P086; -.
DR SMR; Q9P086; -.
DR BioGRID; 134637; 60.
DR ComplexPortal; CPX-3227; Core mediator complex.
DR CORUM; Q9P086; -.
DR IntAct; Q9P086; 39.
DR MINT; Q9P086; -.
DR STRING; 9606.ENSP00000293777; -.
DR iPTMnet; Q9P086; -.
DR PhosphoSitePlus; Q9P086; -.
DR BioMuta; MED11; -.
DR DMDM; 158564056; -.
DR EPD; Q9P086; -.
DR jPOST; Q9P086; -.
DR MassIVE; Q9P086; -.
DR MaxQB; Q9P086; -.
DR PaxDb; Q9P086; -.
DR PeptideAtlas; Q9P086; -.
DR PRIDE; Q9P086; -.
DR ProteomicsDB; 83546; -.
DR TopDownProteomics; Q9P086; -.
DR Antibodypedia; 58423; 33 antibodies from 15 providers.
DR DNASU; 400569; -.
DR Ensembl; ENST00000293777.6; ENSP00000293777.5; ENSG00000161920.11.
DR GeneID; 400569; -.
DR KEGG; hsa:400569; -.
DR MANE-Select; ENST00000293777.6; ENSP00000293777.5; NM_001001683.4; NP_001001683.1.
DR UCSC; uc002fyp.4; human.
DR CTD; 400569; -.
DR DisGeNET; 400569; -.
DR GeneCards; MED11; -.
DR HGNC; HGNC:32687; MED11.
DR HPA; ENSG00000161920; Low tissue specificity.
DR MIM; 612383; gene.
DR neXtProt; NX_Q9P086; -.
DR OpenTargets; ENSG00000161920; -.
DR PharmGKB; PA144596413; -.
DR VEuPathDB; HostDB:ENSG00000161920; -.
DR eggNOG; KOG4057; Eukaryota.
DR GeneTree; ENSGT00390000010184; -.
DR HOGENOM; CLU_123010_2_0_1; -.
DR InParanoid; Q9P086; -.
DR OMA; IAMVLQC; -.
DR OrthoDB; 1616309at2759; -.
DR PhylomeDB; Q9P086; -.
DR TreeFam; TF318328; -.
DR PathwayCommons; Q9P086; -.
DR Reactome; R-HSA-1989781; PPARA activates gene expression.
DR Reactome; R-HSA-381340; Transcriptional regulation of white adipocyte differentiation.
DR SignaLink; Q9P086; -.
DR SIGNOR; Q9P086; -.
DR BioGRID-ORCS; 400569; 808 hits in 1092 CRISPR screens.
DR ChiTaRS; MED11; human.
DR GenomeRNAi; 400569; -.
DR Pharos; Q9P086; Tdark.
DR PRO; PR:Q9P086; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9P086; protein.
DR Bgee; ENSG00000161920; Expressed in right adrenal gland and 183 other tissues.
DR ExpressionAtlas; Q9P086; baseline and differential.
DR Genevisible; Q9P086; HS.
DR GO; GO:0070847; C:core mediator complex; IPI:ComplexPortal.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:ComplexPortal.
DR GO; GO:0000151; C:ubiquitin ligase complex; IEA:Ensembl.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR InterPro; IPR019404; Mediator_Med11.
DR PANTHER; PTHR22890; PTHR22890; 1.
DR Pfam; PF10280; Med11; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Activator; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..117
FT /note="Mediator of RNA polymerase II transcription subunit
FT 11"
FT /id="PRO_0000304308"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CONFLICT 112
FT /note="E -> G (in Ref. 1; AAF28974)"
FT /evidence="ECO:0000305"
FT HELIX 7..34
FT /evidence="ECO:0007829|PDB:7EMF"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 42..72
FT /evidence="ECO:0007829|PDB:7EMF"
FT HELIX 84..115
FT /evidence="ECO:0007829|PDB:7EMF"
SQ SEQUENCE 117 AA; 13129 MW; D01CAA8D211D6FC7 CRC64;
MATYSLANER LRALEDIERE IGAILQNAGT VILELSKEKT NERLLDRQAA AFTASVQHVE
AELSAQIRYL TQVATGQPHE GSSYSSRKDC QMALKRVDYA RLKLSDVART CEQMLEN
