MED12_ARATH
ID MED12_ARATH Reviewed; 2235 AA.
AC H3K2Y6; F4JHC0; O23057;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Protein CENTER CITY;
DE AltName: Full=Protein CRYPTIC PRECOCIOUS;
GN Name=MED12; Synonyms=CCT, CRP, MED12_1; OrderedLocusNames=At4g00450;
GN ORFNames=F5I10.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, MUTAGENESIS OF SER-99,
RP DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=22247249; DOI=10.1093/pcp/pcs002;
RA Imura Y., Kobayashi Y., Yamamoto S., Furutani M., Tasaka M., Abe M.,
RA Araki T.;
RT "CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple
RT target steps in Arabidopsis.";
RL Plant Cell Physiol. 53:287-303(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20023166; DOI=10.1242/dev.043174;
RA Gillmor C.S., Park M.Y., Smith M.R., Pepitone R., Kerstetter R.A.,
RA Poethig R.S.;
RT "The MED12-MED13 module of Mediator regulates the timing of embryo
RT patterning in Arabidopsis.";
RL Development 137:113-122(2010).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors. Flowering regulator which suppresses FLC
CC expression, promotes FT and TSF expression and up-regulates SOC1 and
CC FUL mainly in an FT-dependent manner under long-day conditions.
CC Involved in diverse developmental aspects through gene regulation and
CC modulation of the auxin response. Acts closely together with MAB13.
CC Involved in the regulation of embryo patterning and cotyledon
CC organogenesis by transiently repressing a transcriptional program that
CC interferes with this process. {ECO:0000269|PubMed:20023166,
CC ECO:0000269|PubMed:22247249}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22247249}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Higher expression in vascular tissue,
CC shoot apex and developing floral organs. {ECO:0000269|PubMed:22247249}.
CC -!- DEVELOPMENTAL STAGE: Expressed first at the dermatogen stage of
CC embryogenesis and then in all cells of the embryo and suspensor through
CC the globular stage. Decreases in the periphery of the hypocotyl and on
CC the abaxial side of cotyledons from the heart stage to the torpedo
CC stage, and by the bent cotyledon stage, restricted to the vascular
CC tissue and the shoot and root apical meristems.
CC {ECO:0000269|PubMed:20023166}.
CC -!- DISRUPTION PHENOTYPE: Abnormal embryo and cotyledon development, and
CC embryo lethality in some cases. When viable, plants are small, with
CC abnormal leaf shape and vascular patterning, ectopic bract-like organs
CC and greatly delayed flowering time and sterile flowers.
CC {ECO:0000269|PubMed:20023166, ECO:0000269|PubMed:22247249}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB62842.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF02800.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80854.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB690341; BAL49816.1; -; mRNA.
DR EMBL; AB690342; BAL49817.1; -; mRNA.
DR EMBL; AB690343; BAL49818.1; -; Genomic_DNA.
DR EMBL; AF013293; AAB62842.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF195115; AAF02800.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161472; CAB80854.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE81883.2; -; Genomic_DNA.
DR EMBL; CP002687; ANM67285.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67286.1; -; Genomic_DNA.
DR PIR; T01526; T01526.
DR RefSeq; NP_001319832.1; NM_001340249.1.
DR RefSeq; NP_001329123.1; NM_001340250.1.
DR RefSeq; NP_001329124.1; NM_001340251.1.
DR AlphaFoldDB; H3K2Y6; -.
DR STRING; 3702.AT4G00450.1; -.
DR iPTMnet; H3K2Y6; -.
DR PRIDE; H3K2Y6; -.
DR ProteomicsDB; 238773; -.
DR EnsemblPlants; AT4G00450.1; AT4G00450.1; AT4G00450.
DR EnsemblPlants; AT4G00450.2; AT4G00450.2; AT4G00450.
DR EnsemblPlants; AT4G00450.3; AT4G00450.3; AT4G00450.
DR GeneID; 827954; -.
DR Gramene; AT4G00450.1; AT4G00450.1; AT4G00450.
DR Gramene; AT4G00450.2; AT4G00450.2; AT4G00450.
DR Gramene; AT4G00450.3; AT4G00450.3; AT4G00450.
DR KEGG; ath:AT4G00450; -.
DR Araport; AT4G00450; -.
DR TAIR; locus:2126056; AT4G00450.
DR eggNOG; KOG4522; Eukaryota.
DR InParanoid; H3K2Y6; -.
DR OMA; LTRYNVP; -.
DR OrthoDB; 18330at2759; -.
DR PRO; PR:H3K2Y6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; H3K2Y6; baseline and differential.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0040034; P:regulation of development, heterochronic; IMP:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0090213; P:regulation of radial pattern formation; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR019035; Mediator_Med12.
DR Pfam; PF09497; Med12; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Growth regulation; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..2235
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000418347"
FT REGION 16..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 835..858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1900..1959
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2134..2160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2183..2202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1900..1932
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1940..1958
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2134..2153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2188..2202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 99
FT /note="S->F: Precocious flowering."
FT /evidence="ECO:0000269|PubMed:22247249"
SQ SEQUENCE 2235 AA; 247227 MW; 8B07E6738E94388A CRC64;
MQRYHAANCT SAVNNSAIGG ASARDSGRAD SSSIGNYSLN SRRPPPLTPY KLKCEKDGLN
SRLGPPDFHP PTSNSPEENL TKEYVQFGYK ETVDGLKESE EIILSQVHTF SKPVVHKCKE
AVRKCLRAIN ESRALKRKAG QVYGVPLSGS LLCKPGFPEQ RSCGEETKKR WIESLSQQHK
RLRSLADNIP GYRRKTLFEV LIRNNVPLLR ATWFIKVTYL NQVRPSPAAI SSGTPDKTQA
SRCEQWTKDV IEYLQYLLDE LLSRNSSFPA QQTRDRSPQM LYTGSMQKNS PASTSLYGEE
TSLHFKWWYM VRLLQWHHAE GLLFPNLIVD WVLKLLQEKE IFEILQLLLP IVYGVLESIV
LSQTYVQSLV AIAVRFIQEP APGGSDLVDN SRRAYTLSAL IEMVRYLVLA APDTFVASDF
FPLPPSVAAC GPNDVSYTSK AYENLEKLRS NSAEISAQFQ GRGVLSRFEF LSFDYTISTI
QRSADDLAKI ASAGYPQHNV AKAVQALDKA LSDGDIRAAY SYLFEDLCNG AVDEAWITDV
SPCLRSSLRW IGAISTSFVC SVFFLIEWAT CDFRDFRAGV PKDIKFSGRK DCSQVYLVIQ
LLKQKILGGE FTARKGKNCR NNFLGVSKPS GSMDAFESPG PLHDIIVCWI DQHEVHKGGA
KRLQLLVFEL IRSGIFNPIA YVRQLIVSGM IDVIQPAVDP ERRMRHHRIL KQLPGCFVHE
TLEEAQLFGG DKLSEAVRTY SNERRLLLRE LLVEKGKYWN NLVLSDQKSK KISTSLSSVI
FPRACNAKSN SKGPRKHTKS SVDIRELKER ISALLQFPGM SCGVETPVRD EFQNSVKRSS
GSVYSKMDQP EATPGCEDCR RAKRPKMNDE KSSCYQGNSP IASDEEDNWW IKKGSKTVES
SLKVDPQIEI TKQVPRGRQK MARKTQSLAQ LQAARIEGSQ GASTSHVCDN KVSCPHHGPG
VEGENQKVVD VFRTSTPVDM VSVGNSLKQL QFVDKRSIAV WLTTAVRQLV EEPQKSSVRV
GQFNRGAPVE EKNTIRWKLG ADELYSILFL LDISLDLVSA VKFLLWLLPK ANSTPSFAVQ
GGRNLVTVPR NVENNMCEIG EAILVSSLRR YENILLSADL VPEAMTALMN RAASLMSSNG
KISGSAALVY TRYILKRYGS LPSVVEWHNN FKATSEKKLL SELDHTRSGN GEYGNPLGVP
AGVDNPDDYL RKKISIGGAR PSRVGLSMRD VLQRHVEEAT HYLKKLIGTG TMKASLAEKN
DDGYQVAQQI VVGLMDCIRQ TGGAAQEGDP SLVSSAVSAI INSVGLSVAR ITDFSLGNIY
QNHPSGVDSS NIARYILRIH ITCLCLLKEA LGERQSRVFE IALATESSTA LTGVFAPVKG
SRGQHQLSPE SYDSNANNST IDMSNGTGKM ALSRATKITA AVSALVIGSI THGVITLERI
VGLLRLKDYL DFVQFVRRTK SSSNGSARSM GASKVESPIE VYVHWFRLLV GNCKTVSEGL
VLELVGESSV VAISRMQRML PLKLVFPPAY SIIAFVLWRP FVSNSNSNSS VHEDTHRLYQ
SLTMAFHDVI KHLPFRDVCF RDTQGLYELI VADSTDAEFA SVFESHGLDM HLKSVAFAPL
RARLFLNSLI DCKVPSSGYS HEGVSEAKNR HQGNGTKLVD KLVSVLDCLQ PAKFHWQWVE
LRLLLNEQAL AEKLENHDMP LTDAIRSSCP TSEKPDASEN EKNFIQILLT RLLVRPDAVP
LFSEVVHLFG RSVEDSMLKQ AEWFLAGQDV LFGRKTIRQK LIIVGESKGL PTKPQFWKPW
GWCNSSSSDH ITANKAGKKR KFEITSIEEG EVIEEGSGSR KVLLPRVLDE NSPSVGYGIT
TERAFVQLVL PCIDQSSDES RSTFVNELVR QFSNIEQQLS SVTNRSTTSN KQMGTASSGS
EISSNKGSTR KGLRGGSPSL ARRSSANTTD TSPPPSPAAL RASMSLRLQF LLRLLPVICG
EPSFKNTRHA LASTIVRLLG SRVVYEDYAV CSPRSELSKA ETESTIDPSS MADLSSEVLF
DRLLFVLHGL LSNHQPKWLK PRPSSNESSK DFTLFDRDAA ESLQNELSRM QLPDTIRWRI
QAAMPILLPS LRCSLSCQPH SVPPTALTLV QPSGSTAAAG TNQRNSPAIS KSGTAAAQGK
LKPTMLAPHQ QQEADNTDVV DPWTLLEDGT SSGLSSSNAS NSSDMANLRA TCWLKGAVRV
RRTDLTYVGS VDDDS
