MED12_CAEBR
ID MED12_CAEBR Reviewed; 3902 AA.
AC P0C656; A8WRJ1;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Protein dumpy-22;
GN Name=dpy-22; Synonyms=mdt-12; ORFNames=CBG01900;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; HE601451; CAP23099.3; -; Genomic_DNA.
DR SMR; P0C656; -.
DR STRING; 6238.CBG01900; -.
DR PRIDE; P0C656; -.
DR WormBase; CBG01900; CBP45864; WBGene00025069; Cbr-dpy-22.
DR eggNOG; KOG0018; Eukaryota.
DR eggNOG; KOG3598; Eukaryota.
DR HOGENOM; CLU_000287_0_0_1; -.
DR InParanoid; P0C656; -.
DR OMA; ICEQDWV; -.
DR OrthoDB; 66382at2759; -.
DR Proteomes; UP000008549; Chromosome X.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR SMART; SM01281; Med12; 1.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..3902
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312961"
FT REGION 414..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 619..674
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 694..940
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 977..1029
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1812..1831
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2409..3902
FT /note="Required for nuclear localization"
FT /evidence="ECO:0000250"
FT REGION 2463..2675
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2719..2771
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2876..3151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3195..3549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3563..3902
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2480..2526
FT /evidence="ECO:0000255"
FT COMPBIAS 468..494
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..666
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 709..726
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 733..806
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 820..834
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 870..889
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 890..932
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 977..1005
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2463..2549
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2610..2666
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2719..2746
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2876..2980
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2984..3146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3197..3549
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3563..3644
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3664..3746
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3755..3902
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3902 AA; 446131 MW; 09B67012058A1D14 CRC64;
MGTADVYEQH KNQEEDMLTT ERLKKGYQVP VTHYELQSLI FNASENKLLR LDRAPRRTYC
MISSIVHRRG EYHMNFDSNR KVAKGEMPRF AKIRFKRILE DRVKFFSALF RGTPLQYLQK
KVPYYRRKEL LLLEFYDYQI RYDRAIWCVK LVSILGFNCS AKAQKKALLD LATLEWSQAC
GKVTYYLLLA LRKTYKKSRA AFRESVKMWY YMTRFMMYMF QDGVVDKHEF LNDLTEMFNV
FFIRGSLANA KILELFMEYY IFFLDDLSSS LILARRASSL LCKALGLLSE INEAYTKTQV
YKNENKHEFM GHQDVWTIDA QPKFDFEDYN RDHKEFIDPD DFFRFVDELP DAAYFEDYED
KTAEQVAEEE LQTMEAPRRA RNYIYSALKS VKTPVNDQIP VDLGDELYIP SDVESLTSPE
PEEDPEEGPE EDPRSQSVPS PTFPAGPVSM EEAIPAYWRA RGPVLQTVPQ PAPQPVPKPV
QQTVPKPVPR PPPRNLNIKS EQVDPEYEKN FGNPVKSKNF NINIKAEPMD YDEYSTEPPQ
PLQPPQTFQQ RPKSPEKEKA PEPAAEPPRW TLEELPKNFL SKNLEVPAPW TRPRENPIER
KRAYNLLCAK RFPVKEDVFE PFKDPDSPDG SRPASPIEPI PVKKLHKTPP KKVMTDDDEF
GHIPAQKRKI NPKLANGKYS ETLFVYYLIN GGVAFDPKHD GDEMDFSNPT PPEAPPPPPP
VEFETSPSAS PAPDTDKEDE DGKSKEGGDK AAEKSTDKPA EEKEASEKEK TGEKKDGDKP
TEEKSKKGEK ADGKKAVDEH LNDLIIPMDV EAEAATKRNS ATNSKDDEAE DANKVAGDAP
VVEATHAAGT SEKKPEAEVT PIETNDVEME SVDPATNKES TSDSTAEKSS DSQEPMEVDD
PQKKLPSPAA DKAGDDASKK TKEGKKPDTP AIELVDLEAL DDIPLPTDDL MLPEEDEIQV
IEKPKHPDAD FCEVVEVVEK DKDKTPEKAT EGEKEAEKDA EKLPEPAPVV ESVPEPVKTP
EKPKTPEPPR LYTAEEIEQL KKDKRRQRML DRLTDAFKIQ MESDKKYEEN NIHPSFKVYD
QSRSFRRPLH LLSSMVQYLA FRTPESFVWN NLEVPRPDKC HKILPQLCGS PLDHLPCELH
ELPVMEGNDG HVEHLRLRHH EIVRRSQAAD ENWLPGGADV LQYGRIIDAC VDVIGLMDCI
NVERPNSIFK VSERLFNFRE KFAKEEALLR IKLMFKWCIS EEREGSFRPV YCARMLEYGL
NRSDDGLIGG WQIMDLFFNF MATEGPKPGM ANYQAKFDAC AGIMIEMIRH KLFTVADILR
ELEKDLDIDY TLPLMARQRK QKMPKISKKE KTPEEPDNTK LIHFTTEHTP KRLFMGRKMD
LLERMIIMLP QFGNNKDSDE YKIRNQLLFG LRPSENIYFR RAKTICQGIC KEFDSKSYIE
FNDYVTAHKK LNQSRLDDIL RQFRAQTYHD QHVILEKIVI HLTENIGSFL EKKNGHLPAP
EVVNIICEMY EYSMDITSIF DFFEVLHPYL KAVDEKVASF EMDLVPDMYH TEIAYIFVSF
FMKHYQRFLL HKRNNHIVNQ LYYSVRKMMM DKTRFITCWG RVIAIFVNHI RPVLIDGCFR
IIHIEGNERE FARAHPNKRP FNLECSYFNE EFAGSKLSLR GATMRYDSFA DMKWIVDNLK
VTVKRKNSKP RTPNRYSFVI RAFMEARKHG QNFDRINELA NYCANVTAND PPLSEYWIGA
IKGLCCLCID DSSQLFKEMA KKIDISDCST HYSLSTFITC LAGKAAFYIP RLLHKLTEEV
FSQMLHNDGR LTSHKTKDVK RKSANKTTET RTLSEAEPGV CLSLLIIAGL CCPGDEPFGL
SVHYRGIEKK KKRFRQTADE RIMHMFHWLE MDKSMFRTLA SISKLLEALQ SRCRDANLVL
PQSFQLKHPI VDPNHPPPHE KAPYRPQFLF NIAKAVFFTI CEQDWVTIRM YQFVRINKME
PFNQEKLKTN CLGQQLLRIS LRRKTERSNV HSLFEAHKIS KKATVDKVLS FMNLWNFRAT
LFDLMLMIKE ISPDGNTRHA QQGAIAADAL MSEIGKCCRD MFLAAYKADA KMPNVKKLMD
FRFGDISKYW LLAPLVRMCP KPINIPPQYA NTQVQTVAAK FLREASALMD TPPTTTPKER
LLQCSWAMSK LPFINMILTC LACEKTQSGK DTFLNSVYQQ LQREVFRDHN KRSNWTNRPE
HRDCTVLRVN LIAYMYKEIL KPTHVEVWGL LLYQLMFHGI INPSRERYVF ENCYDMLQHM
VLWTLVNGDN MNSHDRYGSI RVRWPNYAGL MKKIRKEIQD RHVHPSRTCL LRFLPIGKLQ
TSTISYKKYH KRTIRPSATK SKKFMSNEGI RSGKYVYLPE EKVKTNAYDH MKNIPDGIQK
GGWKFRMFQT TRLDKVAKSA LHQLRAKIPH THIGEFKRPC NIMLDNMSED FYLRPPEVEI
TKTVEPVADD EETAAVKKPE EETAEKKKDE AKKADEKTTK ADDEKKKDET ADAKKDNEKQ
KEEKDKPEDS AKAKTDDVTA KDTEKDTAAP TDAAKAAAAP VAAAPDANKE MDTSTPKPAP
VTRETSTTRG RGGGRKRNSG GRAAGGSRAK RTNSRANANA ETAAAAETSS TTKAGPSGGG
SSNYHAAMRA NQPPMSNGSS DETKANIRNL LNRKQEKRNS LADANAAAAV AAAAAAAAAA
AGNIAPALAP IVQPGIPSAG ASSTMPGVPP QQQPHPGMQH QSGMQHQGMG AGMGGMNPTM
HAPPPYSSSS MNPFANQPHF AAPNQVPITN MAHRSTAAVT PEERKRIMEE QMHQKLAQQQ
LLIENQNRQR EAREREAREH QERLHKIREE QMQKEAKIRE EQMQLERQRA IEENNRIMEE
QRILREKEEA RRRMEEEQRR REEEARLAYH VEQERIRKEQ ERQAILEAER LKREEERERE
RQRLEQERLQ REMEEKARRE KEEQARIERE RLERERVARE ALAAQQAQQA QQAQQAQQAQ
QQRHRAMLEQ QQRQQMINQQ QQQQAPYGQQ PPQGQPPSYQ QSSYQNPSGY QQGQQSGQNQ
PPNYQQTSHT NPAIQQTQQP PAYQQDNAQM HMQNRQQQPG QQYGNQPQQQ QSQQAQQGQY
PQQYPQQLSQ NQQMNQQNQQ RNPFGAQPEM QIGGPKVLMQ AKPDEAHAQQ YQHTQAQLAL
SQMEKEKQYL KAKNLQAGQA AGQQQPRFGE NPQQNAPGFN GNLPYPQQQQ QQPQQPGTSQ
IPNTTPTRPA NPMQGQQQQA GMQNYQNQPV LGQPGPMQPG GQAGQQQQNQ FQRQGMHTTP
TKNDMAMRQG QMGGQMGQQQ GQMGQQMQNR PAQGLNQQSQ GFQQQGGQQS AQASFNQPQN
QFSGGQQQQQ MGQNQGQNLQ QGQNQFGRQA APNQENFQQQ PGFNQNAAGQ NYQRPEQQQQ
SQNQWNQLNQ QMRPQQPQQP SQQQQNLPFG RTEQSQQFQQ QQQQPQHQQQ NKPAMGQQGF
NYQGQQQGQG FDQSSQQTQQ HPNPHQQQGH MQQTHQQNAS QQQQQYNQAQ QQSQQQGNQF
PGFSGSSNQA RASNILNQSM EDAGLNQGFS SGNTSNQQAS STNQQGNYGM QQGQQNPMHG
QMHHQQGQQN QNQQQRPGMG QQGMGQQGMG QQGGMGQSGR GQPGMGGQSA MGQHQHGMGR
PTMGQPGMQQ SGMQQQHGMQ QQQPGAQQSG LQQAGMQQQH GGIGMGGMQQ QGRNNYGSMG
QQGQQSGQQQ AQQHQQMSQQ AQPRQMPGGG VQLPPYSMGQ QQQTQQQQPN QQQMDHLRRQ
QQMQQMARQQ AMQQQQQGGV QQGQPQMQGY GNQGQQQQQQ FPQMQQHRGQ GQQGHGMGGA
GQQHQQVPQQ QQNQYFQPQQ QQDQRMQQQP GGQQQQQQGQ SGQQQNNQHY NNMGQFPNQQ
QY
