MED12_CAEEL
ID MED12_CAEEL Reviewed; 3498 AA.
AC Q20497;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=CeTRAP230;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Protein dumpy-22;
GN Name=dpy-22; Synonyms=mdt-12, psa-6, sop-1; ORFNames=F47A4.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=10970880; DOI=10.1101/gad.814700;
RA Zhang H., Emmons S.W.;
RT "A C. elegans mediator protein confers regulatory selectivity on lineage-
RT specific expression of a transcription factor gene.";
RL Genes Dev. 14:2161-2172(2000).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12441291; DOI=10.1242/dev.00189;
RA Moghal N., Sternberg P.W.;
RT "A component of the transcriptional mediator complex inhibits RAS-dependent
RT vulval fate specification in C. elegans.";
RL Development 130:57-69(2003).
RN [4]
RP FUNCTION.
RX PubMed=15073178; DOI=10.1074/jbc.m401242200;
RA Wang J.-C., Walker A., Blackwell T.K., Yamamoto K.R.;
RT "The Caenorhabditis elegans ortholog of TRAP240, CeTRAP240/let-19,
RT selectively modulates gene expression and is essential for embryogenesis.";
RL J. Biol. Chem. 279:29270-29277(2004).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLU-1040.
RX PubMed=15790964; DOI=10.1242/dev.01776;
RA Yoda A., Kouike H., Okano H., Sawa H.;
RT "Components of the transcriptional Mediator complex are required for
RT asymmetric cell division in C. elegans.";
RL Development 132:1885-1893(2005).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Functions
CC downstream of let-60 during vulval induction. Required for asymmetric
CC division of T-cells and for hypodermal development. {ECO:0000250,
CC ECO:0000269|PubMed:10970880, ECO:0000269|PubMed:12441291,
CC ECO:0000269|PubMed:15073178, ECO:0000269|PubMed:15790964}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10970880,
CC ECO:0000269|PubMed:12441291, ECO:0000269|PubMed:15790964}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:10970880}.
CC -!- DEVELOPMENTAL STAGE: Expressed in vulval precursor cells, the anchor
CC cell and the hyp7 cell at the time of vulval fate specification and
CC throughout vulval development. Expressed from the 8-cell stage of
CC embryogenesis. {ECO:0000269|PubMed:10970880}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; Z49888; CAA90064.1; -; Genomic_DNA.
DR PIR; T22330; T22330.
DR RefSeq; NP_509645.1; NM_077244.3.
DR SMR; Q20497; -.
DR BioGRID; 46109; 20.
DR IntAct; Q20497; 1.
DR STRING; 6239.F47A4.2; -.
DR EPD; Q20497; -.
DR PaxDb; Q20497; -.
DR PeptideAtlas; Q20497; -.
DR PRIDE; Q20497; -.
DR EnsemblMetazoa; F47A4.2.1; F47A4.2.1; WBGene00001081.
DR GeneID; 181194; -.
DR KEGG; cel:CELE_F47A4.2; -.
DR UCSC; F47A4.2; c. elegans.
DR CTD; 181194; -.
DR WormBase; F47A4.2; CE16058; WBGene00001081; dpy-22.
DR eggNOG; KOG3598; Eukaryota.
DR GeneTree; ENSGT00440000037505; -.
DR HOGENOM; CLU_000287_0_0_1; -.
DR InParanoid; Q20497; -.
DR OMA; ICEQDWV; -.
DR OrthoDB; 66382at2759; -.
DR SignaLink; Q20497; -.
DR PRO; PR:Q20497; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001081; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; IGI:WormBase.
DR GO; GO:0040027; P:negative regulation of vulval development; IGI:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Developmental protein; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..3498
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312962"
FT REGION 365..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 497..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2142..3498
FT /note="Required for nuclear localization"
FT REGION 2209..2576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2589..2620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2637..2889
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2917..3498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 539..570
FT /evidence="ECO:0000255"
FT COILED 2203..2290
FT /evidence="ECO:0000255"
FT COILED 2395..2420
FT /evidence="ECO:0000255"
FT COMPBIAS 375..391
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..437
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..538
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..582
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..621
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..636
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..651
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 673..724
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..764
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2209..2284
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2290..2310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2345..2368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2377..2392
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2393..2409
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2439..2496
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2514..2528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2534..2576
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2637..2721
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2722..2854
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2868..2885
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2928..3011
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3026..3498
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1040
FT /note="E->K: In os26; impairs asymmetric division of T-
FT cells."
FT /evidence="ECO:0000269|PubMed:15790964"
SQ SEQUENCE 3498 AA; 400779 MW; 283FFC65A6B9E336 CRC64;
MLRKTNLRMG TADVYKQNKN QEEDMLTTER LKKGYQMPLT QYELQSLVFN ASENKLIRLE
KAQKRSQSMI ASVIHKKGEF HMNYDRERKI PKEQLPCFAP HNWKRILSDR VKFFSALARG
TPLQQLSQRI PGFRRKELLF LEFSDYNISY DRAIWCVKLL CILGFNSSSK TQKKTLLDVA
TLELSQACNK TTIWLVTQLY KSFRNPIKRR EATKTWNYLT GFMKYMFDDG VLDKQEFLND
LTEVFNQLFL QKNFKNPSVL STFMKYYVYF VEDLSSSLVL ARRSSTLLCQ ALGFLFEMAQ
DKRKIDAYYD SANKTFIKKE FENEDGEIYE FEDYDDFIDM LDEIPDVPYF DETDKTPEAQ
AEELIKQHEK RKAGSLKKSE RRRRRGLSKN RPKKMPQNPS NEASLDHDKV QIKQEPMDYD
EYGGQQSDNS MDYDSFSHQY DPSYEPLPFK PLHTDIPKKL TDDEEFKYMK IGKRSVTPER
ISGRYGSMTY PFWIQQGGVG IDPPKDEVLD FSNPTPTETV NTQPQIDFES SPSASPALSV
DKENECEKKE DESKTKEKNK DKEKDKEKEK SVDEHTNDLD VPINPDDAEM ADANDKTDAS
EKQKLVEEEP TGKENEDDTS SKTAKTSTSA EKSEAPSIVD SNDKIDKEPN ASSTSNDETS
KDDTVPMESD PPAATEKPKE STEITTEEPL EVDKAPEVDK SEKEHEDDIM IIESNKKADE
DDCVVIAVVD PDQEQTPESE KKKDGEEERD KNKDTDVADN EPEKIYTDEE LAEIKIRKEK
EMKIATLASH IKEKMVSDKK WKEKTSHAGW RTLDQCSQFS EALHLLSSMV QYMACVTPES
FVWNDLSVQQ EERRHRILPQ LCGSPLDYLP CELHKLPVME GVEEVVDCLR LRHCEIVRRS
QAAEDRWLPN AAFLQSFGRI IDTCVDVIGI MDNIDVEKPN AITNAGLRLF AFREKFEKQE
ALLKTMLMFK WCITEEREGS FRATYIAKLL RFGMDQNPEN TIGGWQVMDL FFKFMSTEGP
KHGSKMYQAH FDSTVAIMIE MMREKIFSIT DILRELEKDS DLDYNAPLME RQRKQKIPKI
SKRHRKPETP DDTKLVHFTT EYTPKRLFMG KKMDLLERMI IILPQLDVDE DTDEYRLRRL
LLFGLKPAAN VYFRRARAIY KSITKEFTTR LYIEFDRSSK VTTAHKKINQ NRLDDLLRQF
RAQTYHDQHL ILERIVYNFI DGIGGFLKKN CDDVPAPEVA NIICEMYEFS MDITSIFDFF
EMVNPYLKAV DDKIAHFRMD VLPDMYYTET AFIFVSFFMK HWQRFLLHPR ACAIVNQCFV
LIQDMIRADD HMITCWGRTV AIFVFHARKA IANAGLQNEE FLAEDSHFWR VFPNAQHVDL
DVGYFNEDFA GVKLQLRGGT LRYDSYNDFK WLVSNMKPNL KKKPHLKRPN TRYSFVVRAF
MEARQHGRNF DRINELANYC ANITANDPPL SEYWIGAIKG LCFLSLDAPY PFKEMSQQID
ISDCSTHYSL TTFITCLAGK SAFYIPRLLA ELTKHVFPLM LRHDGRLTSQ KTHDVKRKVA
SKTTESRTLS EAEPGVCLCL LIICGLCCVG DEPFGLSVHY RGIEKKKKRF NNTADERIMH
LFHWFEMDHA MFRTLGHISQ LLEALQSRCR DANLVLPKNF PIKNPPKHQQ ELHREKAPYR
PQYLFNIAKT VQFVICEQDW VTLRMFRFFQ TRKMEAFNQD KLKQNCLGQQ ILRMALRRRT
ERENVHKLFE AHKISKKATV DKVLSFMNLW NFRATLFDLM LMIKEISPDG NSRHAQQGAI
AADALMSEIG KCCRDMFLSA YKTKIKMPIA KTLTDFRLSD INKFWLIAPL VRMCPKPINI
PPQYANTTVG TVAAKFLREA ALLMDTPPTT PKERLLQCSW AMSKVPFINM ILTCLQCEKM
QQSKDVFLQS LYTQLQRETL RDHHRRSNWT NRREHRDCTI FRITLIGYIY KEILKATHVE
TWGLLIFQLM FHGIINPVRE KLIYETCFDM LHHMVLWTLV DGDSMNQHDR YGSIRVRWPQ
YAGLMKKIRK EMQERFTDQT RNSLHRFLPI GKMQMSTISY KKYQKRPKVN QKMSKKFLAG
EGLKNGKYSF LPEEKAKTNA FEHTDHLGDL IVKGGWKFRM FQTTRLDKVA KNVQNVLRSN
MHHTHVLEFN RPQLLMSDNM FDDIFLAPPD IEITKIIEQP VPVIDEEEAK KRAEEEKEAA
EKKEESKNAE DEKNKNNAEN KKDTKEGEKG KSKDKEKDGE KEKCKDASKK DDVTSEKNEL
EKRASDAAAA TNAPETNKDM DTSTPKPAPV TRSPATRGRG GGRKRNSGAR GGGPRAKRAN
SRADTAQAAA ATTQWNAPIA NTSNPAAGGN FHAAMRGNQP PMSNGSSDET KVHIRNLLNR
KKEEKRNSLA DASAAAAAAN SNAMGNTSSM PPSGPPMPMG SSMQSAGATQ QLQGMQKHQM
GGSMSGMNQN MGGMNQSMSH QAPPPYSSTN EMNRPLMNQY GGPHFAAPNP GPLNRSSGPV
SSETRQQIME QQMREKLAAH HQLVEQQKQR DAREREARER EAREHQERMQ REAYMKEQQL
LERKRAIEEN NRIMEEQQRE REMEAARKEA ARRAAEEAYA AEQQRLELLR RQEEERLRKE
AEERMRIQRE NEERVRQEQM RLEAEERERI RRAEEERIQK ELEDKVRREK EEAARQEKER
QEQEARMREA REAELSRQRM EQQRRSQQNP YMNQQGQYSQ QPPPSYQQSS YPNNYQPGQQ
GNQPPNYQQP SHQSMQQGHQ AGYQQTSNQM QMNMQQQQNR QQGGPQQSFS GPGGINQPSQ
PGYSGYNQQG GQGQQGQMQQ QRNPFGNQQD MQQPGAAKLM HAKPNEAHAQ QYQHTQNQLS
LAQKEKEKQY FQAKNLQASQ ANAQQQQQRF GDVVAGNVAG YGRPYGQQQL GASDQMGTSQ
LPGASTSRMN QGSSNPQGGM QSYQQQQPVL GQPGPIQTGQ STQQQIPAQS QQQYNSGRPQ
MHTTPTKNDM SARAPSGAMG QIANRMGHGT NPQGYGSTGQ NVPGGYQQGQ QQSGQGSYPQ
AQQQQPNQYS GSNQQVGQQA QQQQQQPLNQ NVSQSQSAAQ FGRPSQDSAY QQSGYNQTGN
QSYQRPDQQQ QGAQQNQWSG SNQAQNQLRS QQQAQQPLQQ PQQSQQFQQP AQQAKNPMAQ
SAQYGGFGGQ QQGYDQQQQG QIAPQQAQNP QASQSYGQQQ TQQNRYGMGS SGYTANSGGS
SNILNQSMEE SGLNQGFSGA SSNASSQQGG SSQMQQSGYG MPGNQMQMQQ NQKQQVQRGM
PTGMGQTNMG QSGMGQSGMG QTGMSRSGLG GGIGQQGQQS QQPQQPQVSQ QQNQRGMNPG
AQLPPYSTGQ QQHQPQQSQI SQQQQQQDQY RRMQAAQMQQ QPTAQGQQNR MGMPSQQQSG
AAYSNQMQFQ GVRQGQQGMG GMGGSGQQQP QTQPHGSNQY YQQQQDQRMQ QQPQQPGQQQ
QHGYGMGQYP NQQPPNQY
