MED12_DANRE
ID MED12_DANRE Reviewed; 2173 AA.
AC Q2QCI8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Protein kohtalo;
DE AltName: Full=Protein motionless;
DE AltName: Full=Thyroid hormone receptor-associated protein complex 230 kDa component;
DE Short=Trap230;
GN Name=med12; Synonyms=kto, mot, trap230;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16344459; DOI=10.1073/pnas.0509457102;
RA Hong S.-K., Haldin C.E., Lawson N.D., Weinstein B.M., Dawid I.B.,
RA Hukriede N.A.;
RT "The zebrafish kohtalo/trap230 gene is required for the development of the
RT brain, neural crest, and pronephric kidney.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:18473-18478(2005).
RN [2]
RP FUNCTION.
RX PubMed=16712834; DOI=10.1016/j.ydbio.2006.04.437;
RA Rau M.J., Fischer S., Neumann C.J.;
RT "Zebrafish Trap230/Med12 is required as a coactivator for Sox9-dependent
RT neural crest, cartilage and ear development.";
RL Dev. Biol. 296:83-93(2006).
RN [3]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17088561; DOI=10.1073/pnas.0605414103;
RA Wang X., Yang N., Uno E., Roeder R.G., Guo S.;
RT "A subunit of the mediator complex regulates vertebrate neuronal
RT development.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:17284-17289(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC development of the body axis, brain, ear, kidney, forelimb and neural
CC crest and for pigmentation. Acts as a coactivator for sox9a and/or
CC sox9b promoting the expression of several neuronal determination genes.
CC {ECO:0000250, ECO:0000269|PubMed:16344459, ECO:0000269|PubMed:16712834,
CC ECO:0000269|PubMed:17088561}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Maternally expressed. Widely expressed in the
CC early embryo and concentrated in the head by 48 hpf.
CC {ECO:0000269|PubMed:16344459, ECO:0000269|PubMed:17088561}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; DQ133567; AAZ73481.1; -; mRNA.
DR AlphaFoldDB; Q2QCI8; -.
DR STRING; 7955.ENSDARP00000124576; -.
DR PaxDb; Q2QCI8; -.
DR PRIDE; Q2QCI8; -.
DR ZFIN; ZDB-GENE-060125-1; med12.
DR eggNOG; KOG3598; Eukaryota.
DR InParanoid; Q2QCI8; -.
DR PhylomeDB; Q2QCI8; -.
DR Reactome; R-DRE-212436; Generic Transcription Pathway.
DR PRO; PR:Q2QCI8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0008013; F:beta-catenin binding; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IGI:ZFIN.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0021954; P:central nervous system neuron development; IMP:ZFIN.
DR GO; GO:0043583; P:ear development; IMP:ZFIN.
DR GO; GO:0048702; P:embryonic neurocranium morphogenesis; IMP:ZFIN.
DR GO; GO:0035118; P:embryonic pectoral fin morphogenesis; IMP:ZFIN.
DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; IMP:ZFIN.
DR GO; GO:0007492; P:endoderm development; IMP:ZFIN.
DR GO; GO:0030900; P:forebrain development; IMP:ZFIN.
DR GO; GO:0021986; P:habenula development; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0050935; P:iridophore differentiation; IMP:ZFIN.
DR GO; GO:0001822; P:kidney development; IMP:ZFIN.
DR GO; GO:0030901; P:midbrain development; IMP:ZFIN.
DR GO; GO:0014032; P:neural crest cell development; IMP:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IMP:ZFIN.
DR GO; GO:0021982; P:pineal gland development; IMP:ZFIN.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IGI:ZFIN.
DR GO; GO:0002761; P:regulation of myeloid leukocyte differentiation; IMP:ZFIN.
DR GO; GO:0021654; P:rhombomere boundary formation; IMP:ZFIN.
DR GO; GO:0021591; P:ventricular system development; IMP:ZFIN.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021989; Mediator_Med12_catenin-bd.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR Pfam; PF12144; Med12-PQL; 1.
DR SMART; SM01281; Med12; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..2173
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312960"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 630..718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 784..804
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1380..1404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1443..1467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1737..1780
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2020..2068
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..659
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 661..683
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 789..804
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1443..1464
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1743..1757
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2027..2068
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2173 AA; 243992 MW; 9BE52FEAAEB97F88 CRC64;
MAAFGVLSYE HRPLKRPRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA VSGDEHGSAK
NVNFNSSKIS SNFSSIIAEK LRCNTFPDTG KRKPQVNQKD NFWLVTARSQ SSINNWFTDL
AGTKPLTQLA KKVPIFSKKE EVFGYLAKYS VPVMRSAWMI KMTCAYHAAI TENKMKKRHV
IDPCIEWTPI ITKYLWEQLQ KVAEFYRQSP SQGCGSPLPA PPAEVETAMK QWEYNEKLAM
FMFQDGMLDR HEFLTWVLEC FEKIRPGEDE LLRLLLPLLL QYSGEFVQSA YLSRRLAYFC
TRRLNLLLSD GSIGPGPGGH QAHGISAQQG NALPPTPTSQ PAGGNQPQTP FTDFYICPQH
RPVVFGLSCM LQSIVLCCPS ALVWHYSLTD SRNKTGSPLD LLPIAPSNLP MPGGNSTFNQ
QVRAKVREIE EQIKERGQAV EFRWSFDKCQ ETTAGFTISR VLHTLEVLDN HSFEKSDFSN
SLDSLYNRIF GSGQSKDGHE MSPDDDAVVT LLCEWAVSCK RSGPHRAMVV AKLLEKRQTE
IEAERCGESE VVDEKGSVSS GSLSAATLPV FQDVLLQFLD TQAPVLTEPG NESERVEFSN
LVLLFYELIR HDVFSHNIYM CTLISRGDLA SNSHLPRPRS PSDEPSDESE RKDQDAGSSV
KMEDTGMSES MEIDHNSSAN FDEMFSPPMH CESKGSPSPE KQAQEQESKS TAKDKGMDPA
FPLVYEQPRH IQYATHFPIP QEESASHECN QRLVVLYGVG KQRDEARHAI KKITKDILKV
LNRKSTAETG GEEGQKRKRS KPEAFPTAED IFSKFQHLSH FDQHQVTSQV SRNVLEQITS
FALGMSYHLP LVQHIQFIFD LMEYSLNISG LIDFAIQLLN ELSLVEAELL LKSSNLAGSY
TTGLCLCIVA VLRRYHSCLI LNPDQTAQVF DGLRIVVKSG VNPADCSSAE RCILAYLYDL
YTSCSHLKSK FGEIFSEFCS KVKNSIYYNI DPSDSNMLWD QMFMIDAITN PTAHNLNHSM
LGKITNELND SPANRYSFVC NVLMDVCVDH RDPERVNDIG ILCAELTAYC RSLSAEWLGV
LKALCCSSNN GNCGFNDLLC NVDVSDLSFH DSLATFVAIL IARQCLLLED LVRCVAIPSL
LNAACSEQDS EPGARLTCRI LLHLFRTPQR NPCPQDGTKS DKSIVGIRSS CDRHLLAASQ
NSIVVGAVFA VLKAVFMLGD AELKGSGFSH PAGLDDIGED DMGSKKSGGR NVSIETASLV
VYAKYVLKSI CHQEWVGERC LKSLSEDSSA LQDPVLVNIQ AQRLLQLICY PHRQLDSEEG
DNPQRQRIKR ILQNMDQWTM RQSSLELQLM IKQSSNNELY SLLENIAKAT IEVFQKSAEM
NSSNPSWNGS AVSGSSVSNS NSASKLKPVL SSSERSGVWL VAPLIGKLPT SVQGHVLKAA
GEELEKGQHL GPSSRKERDR QKQKSMSLLS QQPFLSLVLT CLKGQDEQRE GLLTSLYSQV
QQIVTNWRED QYQDDCKAKQ MMHEALKLRL NLVGGMFDTV QRSTQQTTEW AVLLLDIISS
GTVDMQSNNE LFTTVLDMLS VLINGTLAAD MSSISQGSME ENKRAYMNLV KKLRKELGDR
QSESLEKVRQ LLPLPKQTRD VITCEPQGSL IDTKGNKIAG FEKEGLQVST KQKISPWDVF
EGLKHSAPLS WGWFGTIRVD RKVTKFEEQQ RLLLYHTHIK PKQRSYYLAP LNLPQEEEEP
LTPVPPEPDK KLDTAKVEKS VSNVPTKKKK KKPASTVKQE DYKPHNTVMQ YAPGMTPDLP
LSMGQTNMSY RMGYQAPMNM YAQNQPLPPG GPGLEPPFRP RVPMNKMPPR PNYTMPNMQT
AGMGNLMGME KQYQMNYKPM PNMAQGQMLR HQLQGHNHLI GQQIRQVTPN PQYSSMQPAQ
NIPQGYTSYG SHMGMQPHPS QTPGMVPNSY GNQGFQTGHP ATNPTMVDSI RQMQQRPGYV
HQQAPAAYGH TIQSTQRFPH QSMQQTPMMH GLAQSHLGVQ GIHPNMRPNQ MMEQQQQQQQ
QQQQQQQQQQ QVHQQQQHIR QGPVFRPQQQ QQVQQQVQQQ VQQQQVQQQQ VQQQVQPQQV
QQQQVQQQQQ QQVSAAQPPA QALGMQALPP QQPMFQRQGM QQTQQQQQTA ALVRQLQQQL
SSTQPAQNNS SYY
