ID   MED12_DICDI             Reviewed;        2731 AA.
AC   Q54U49;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 2.
DT   23-FEB-2022, entry version 78.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Putative mediator complex subunit 12;
GN   Name=med12; ORFNames=DDB_G0281277;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000040; EAL66935.2; -; Genomic_DNA.
DR   RefSeq; XP_640927.2; XM_635835.2.
DR   STRING; 44689.DDB0266871; -.
DR   PaxDb; Q54U49; -.
DR   PRIDE; Q54U49; -.
DR   EnsemblProtists; EAL66935; EAL66935; DDB_G0281277.
DR   GeneID; 8622990; -.
DR   KEGG; ddi:DDB_G0281277; -.
DR   dictyBase; DDB_G0281277; med12.
DR   eggNOG; KOG3598; Eukaryota.
DR   HOGENOM; CLU_227164_0_0_1; -.
DR   InParanoid; Q54U49; -.
DR   OMA; NDWTKTI; -.
DR   PRO; PR:Q54U49; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..2731
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 12"
FT                   /id="PRO_0000388652"
FT   REGION          29..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          101..145
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          310..376
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          432..451
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          685..708
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1251..1341
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1778..1825
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1892..1958
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2212..2258
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2307..2351
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2472..2532
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2705..2731
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          5..37
FT                   /evidence="ECO:0000255"
FT   COILED          75..108
FT                   /evidence="ECO:0000255"
FT   COILED          141..189
FT                   /evidence="ECO:0000255"
FT   COILED          275..304
FT                   /evidence="ECO:0000255"
FT   COILED          1316..1344
FT                   /evidence="ECO:0000255"
FT   COILED          1375..1433
FT                   /evidence="ECO:0000255"
FT   COILED          2239..2270
FT                   /evidence="ECO:0000255"
FT   COILED          2336..2363
FT                   /evidence="ECO:0000255"
FT   COILED          2523..2662
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        310..337
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        349..363
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1251..1270
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1278..1327
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1783..1825
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        2212..2254
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2731 AA;  317067 MW;  6C8B6D237824C947 CRC64;
     MMNPQQILQQ QQQMIQQQQQ QLHQQQMQQQ QMQQQQQHHM QQQQPMQHMQ QPHHMIHQQQ
     PIIIQQHLHH QIHPHIQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQVHPS HLQQQVHHPQ
     LHMQQHIPQH MQQHHIMQQH QQIHQMQMQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQHQQQQQP IQQQHIVQQQ PIQQQPIQQH IVQQPIQQQI QQQPQIQQQQ IPQPQIQIPS
     QIQQPLQQQQ IQQQQIPLPQ QVPLAQTQQQ QTAPIQQLQQ QQQLQQQQQQ QQQQQQQQQQ
     QQQQPQQVQQ PQQQQQQQQP VARRPIQNPS YHSQSQIGHP MRGGGLKKYE IQKPADKKEL
     GPDLGVPDFY PLDDKSKEDT LPQEFILGGF CERYQHDECK SSSSIFKNFD FPKVKEQLLQ
     SIYQVEWKKS STQKSGQPPV MNKSGNKRPV PEQKAWSANH RESWVLKLAG NEPLQKLASS
     VPHGYKGETL LKMFLDNQVP LIRATWYTKI VYGNMPKHKT VEPSNDWTKT IVQSLFALIR
     NTTGENNSSG SSSSNNVLGG NSGSGVVKKA TSSYTGILYI ERLIKWNFSE GLLNKDILLS
     MLIDHLNETD RPEEAVLISS LLLYYSDTLI LSTYHSMKFL NKAYEKLMKI TISSAAAVSA
     ASSSSSSSST QSNITALSNP KLQQLGHGHG HGHGHHSHSH SHNQQQPQLN ITSTQTIPSI
     NSIASSLSPS NQKIYSILCI ISKQISMNLP DTFMSFKFFR ELVSLIWPSN LIDRIEYNQL
     SFVNNLRFTV SPEYKWINKY YSEINKNQQT QLKQLRQQQN NNIGFKNNKN NNSIINKQNN
     ITNNNNNNNN NSRGGINYSN LIKSLDRFYT TFDLKLLYHE IFGNGNQVSL EDDKEKILLV
     CEWACTSCRN YPFSFLSATS ILKIFERNLT IKLNNIVLNH PLQNILVHFL ETFKPKNIEL
     KRICQLFSEL IRNEIFSQNS YARYLISRGI LENSNINING NHNGDKNNNI NGRLENNKYH
     KYYLKEFPIF NRNDFPSYDI HQQRNQRRTI LFPNTIKSQE EINSMEQARI SVKSFILNSA
     NSNNENDNNS DMMVDGGGGG GGGDKQNIGL IQLSQIIQSL SFYSQISLTE WLSLCLRLNF
     NNPSNDDNNN NNNNNNNHHQ LSDYSMKGFR HFSFPITQFY IERVFIIMES VSNYHSILQC
     LLVLWDNWNN IKELQSFVFY TTKRLQQAFI VSDQFISLLD IIYKKVINLS RNNNNNNKNK
     NNNKQSNNIK KEGTNGEEQE SDDDDDDDED DDDDDNDEND KVNDNDNEED DEEDDEDEDE
     DEDDQMDETK DSIKQQQQQK SNENYIESYL YDILNKFKSI KSVQIWIKRN NVQCKLKKEL
     SNDKNMIDKS ELKNSCDFMI DQVLSIKNQL KQEQEQQNCE IINNIIKRFD EELISNNLIN
     SLMNQFKTKV SNILLGCENG GSGGGGGGGN LSDQDICEEF MKSLISKTID DNKNNDNNNN
     NENENNNIIS KTIIIIKQQM VLSRLLCLFS IVYPISMNGK QLFLRLFKDQ LSIILKTFIN
     SNDSMEIQEK YFFKVILLLV TLILNQYIDL YSIMVYSILP LLQSNEIESD EDCKVWFLIK
     IIQVLLCDGK LIQFVYKDIF IDNNNNNHQS NQGGGGDSGN NNLTNLKTII NHQVISLNLT
     NKSKISSLSE IFQTICKLYH QYHEKIKDTI DDDNNNNNSK NELSTSIFQG ICTILTDPDS
     RYIFLNSSQI DKIKQLGLGP IETRYLFHLI LGNLKNDHDD NNENNNNNNN NNNNNNNNNN
     NNNNNNNNNN NNNNNNNNNN NNNNGFNFKI NNSRIIIQYE QLKLENCITY SFKVINSTLT
     NSSNCYWNIH LSYIGLKLLL DEWKTLPPII STQSSTTSPL TKSPSQSPTL QSQDLNSTNS
     TSPTSKQSQQ QQQQQQQKST TPTLTPATTT TTTTNNTTTF TPESIITQFI LLHFEENRGW
     ERVFIYEDLF LLFPLEVRNE LIKISTIILE SQYKETSFIQ PSTDLPLIKH IQQNLPISQL
     LKQQPQQYQL ILNRESSGTN KAVLINDDNL DNILFQESFT DIIINILSTY EDSNPISNSF
     ILSLFKQLSM FNQFSKGGVL DTINLDSTLS EIVIQSVVIR ITFLITQIKN CKLIVKQSTQ
     KLEEISVTLL NLLSKFIIQS DGEFNLFDPI LTIFDLILSD KFGNDNILNY SSSSSSTTTT
     STTSTPVTSS PSTTTTATTN QQQQQQQQQQ ADQKNNVKKK LHELYQKIKS SFPTSLQLKL
     EKQLSFLSTN PTSLPFTIIH PPTLVNTSSV SSTNTSIQST TSPIIAPVTP PSNFNLQQQT
     SQQQQQQQQQ QQQQSQQHQQ QQQTPQQQQQ SQQQQQQQQQ QTATTASTAS QIPPQAVSSI
     LDIKPTYTQM DPWTLLEDYS ETPLSPSIYG GVKMERKELT YIKSSFPSKN MALVNEPVDQ
     KLLAQRKSQV QEIEKQQQQQ QIKSQPSTPL TSLPPQQHQQ LQPPPPQLQQ QLNQQQQRQQ
     PPQQLQQQKP QTQQQQQQQQ QQQQQQQQQQ QQQQTQKQPQ QPQQPQQSQQ FQQYQQFEQQ
     QQALQFHLNQ QKMQQLQPQQ LNQQQLNQQQ LNQQLNQQQQ LNQQQFQLHQ QQQQQLKQQQ
     LQQQQLQQQI QQQQQQQQLQ QLIQPQQFQQ QNLKRNFNNL SQPPLPQQQQ QQQIHLQQQQ
     IPQQHQQIPQ QQQHQQHQQQ KCSTTKYNNY K