MED12_DROME
ID MED12_DROME Reviewed; 2531 AA.
AC Q9VW47; Q8MQW4; Q9GPH4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Mediator complex subunit Kohtalo;
DE AltName: Full=dTRAP230;
GN Name=kto; Synonyms=Med12, Trap230; ORFNames=CG8491;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=11171343; DOI=10.1242/dev.128.4.603;
RA Treisman J.E.;
RT "Drosophila homologues of the transcriptional coactivation complex subunits
RT TRAP240 and TRAP230 are required for identical processes in eye-antennal
RT disc development.";
RL Development 128:603-615(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 964-2531.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, INTERACTION WITH SKD, AND SUBCELLULAR LOCATION.
RX PubMed=12835386; DOI=10.1242/dev.00607;
RA Janody F., Martirosyan Z., Benlali A., Treisman J.E.;
RT "Two subunits of the Drosophila mediator complex act together to control
RT cell affinity.";
RL Development 130:3691-3701(2003).
RN [6]
RP FUNCTION, AND INTERACTION WITH CYCC; CDK8 AND SKD.
RX PubMed=17290221; DOI=10.1038/sj.emboj.7601566;
RA Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs D.L.,
RA Bourbon H.-M.;
RT "Distinct roles for Mediator Cdk8 module subunits in Drosophila
RT development.";
RL EMBO J. 26:1045-1054(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-781, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-745; SER-748; SER-806;
RP SER-1356 AND THR-1360, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for leg and eye
CC development and macrochaete specification or differentiation.
CC {ECO:0000269|PubMed:11171343, ECO:0000269|PubMed:12835386,
CC ECO:0000269|PubMed:17290221}.
CC -!- SUBUNIT: Component of the Cdk8 module of the Mediator complex, composed
CC of CycC, Cdk8, kto and skd.
CC -!- INTERACTION:
CC Q9VW47; P25008: CycC; NbExp=2; IntAct=EBI-139710, EBI-195485;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12835386}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM52769.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF324426; AAG48328.1; -; mRNA.
DR EMBL; AE014296; AAF49103.2; -; Genomic_DNA.
DR EMBL; AY122257; AAM52769.1; ALT_FRAME; mRNA.
DR RefSeq; NP_524786.1; NM_080047.2.
DR AlphaFoldDB; Q9VW47; -.
DR BioGRID; 69311; 14.
DR DIP; DIP-29990N; -.
DR IntAct; Q9VW47; 9.
DR MINT; Q9VW47; -.
DR STRING; 7227.FBpp0074653; -.
DR iPTMnet; Q9VW47; -.
DR PaxDb; Q9VW47; -.
DR PRIDE; Q9VW47; -.
DR EnsemblMetazoa; FBtr0074884; FBpp0074653; FBgn0001324.
DR GeneID; 44830; -.
DR KEGG; dme:Dmel_CG8491; -.
DR CTD; 44830; -.
DR FlyBase; FBgn0001324; kto.
DR VEuPathDB; VectorBase:FBgn0001324; -.
DR eggNOG; KOG3598; Eukaryota.
DR GeneTree; ENSGT00440000037505; -.
DR HOGENOM; CLU_000904_0_0_1; -.
DR InParanoid; Q9VW47; -.
DR OMA; YQQSHDK; -.
DR OrthoDB; 20034at2759; -.
DR PhylomeDB; Q9VW47; -.
DR SignaLink; Q9VW47; -.
DR BioGRID-ORCS; 44830; 0 hits in 3 CRISPR screens.
DR ChiTaRS; kto; fly.
DR GenomeRNAi; 44830; -.
DR PRO; PR:Q9VW47; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0001324; Expressed in second segment of antenna (Drosophila) and 23 other tissues.
DR ExpressionAtlas; Q9VW47; baseline and differential.
DR Genevisible; Q9VW47; DM.
DR GO; GO:0070847; C:core mediator complex; IPI:FlyBase.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:UniProtKB.
DR GO; GO:0022416; P:chaeta development; IMP:FlyBase.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0035072; P:ecdysone-mediated induction of salivary gland cell autophagic cell death; IMP:FlyBase.
DR GO; GO:0036011; P:imaginal disc-derived leg segmentation; IMP:FlyBase.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045498; P:sex comb development; IMP:FlyBase.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..2531
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312963"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 584..604
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 742..762
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 796..824
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1585..1608
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1898..2092
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2114..2218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2469..2508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..253
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1936..2054
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2066..2092
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2114..2209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 745
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 748
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 781
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 806
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1356
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1360
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 2531 AA; 279499 MW; 68B0CD469E83A9F5 CRC64;
MLSMLQEKRP LKRTRLGPPD IYPQDAKQRE DELTPTNVKH GFTTTPPLSD EFGTAHNSNV
NASKVSAFFS GVLAKKEELM TLPDTGRKKQ QINCKDNFWP VSPRRKCTVD AWFKDLAGNK
PLLSLAKRAP SFNKKEEIFI TLCENQVNMQ RATWFIKLSA AYTLSFTESK NKKRSIYDPA
AEWTGNMIKF MKELLPKLQE YYQQNHDKSS SNGTTSGSLT AAGNGPASNG STGTSSINSV
TGSSASTNVI PVPSMASPLP PIHSPANGQQ AAPGGGVNAG SVMPTTGSLG GVVGGPGSSV
VGGAAGAGAA VPPGSTISGI GSQFEDSRNA LKYWKYCHQL SKYMYEESLL DRQEFLNWIL
DLLDKMRTQA SFDEPLKKLV LSFALQYMHD FVQSERLCRK MAYIVSKKLA QLLNTVVEQQ
TIKELDEPKL QQDPYELALQ EQMSCPHHRD IVLYLSTILQ IITIECPTAL VWSGIAAHRA
PSSLLGSPLD HLPLAPSVLP MPTRCPRTNH EIRRQLRAAE SDIVLRTQHA EQRWFAAKWL
SAGKNQYTSV LATLDHLDTH CFDRMEHNNS IDTLYAQIFP SPTVSRRREE DQVEPRPPYE
PKQDKDTVRI LCEWAVSGQR WGEHRAMVVA ILLDKRQIDV TSTPADQQSS DKDDKDSLAS
GAGLIDGLPV FQHVLMHFLD HDAPVLDEHV SSPQQRTEFT NLVQLFSALI RHDVFSHNAY
MHTLISRGDL LLESVLVIKS GTTATKTSPP PPAPPPTTTH GFDDDGFGGG LDFKHNEFDD
SNVDDDLDKL VQNIKEKGQQ HEAPDSPKIG PPGDGETNPG GSISRHYVYT KHFPIPQDDP
SMSSYSSESN QRYILLFGVG KERDEKKHAV KKMSKEIGKL FTKKFSIDVA AAGHVKKHSR
NEFNFEATTS KCQQMAYFDQ HVVTAQCAAN VLEQLNGFAL GNNNYLPVQE HVAFLFDLME
LALNIYSLLE LCDSLLKELP EVEHQLQLKK SNLVRSYTTS LALYIVSILR RYHSCLLLSP
EQTLSVFEGV CRTIRHVSNP SECTSAERCI IAYLSDLHES CVLLQGKEQS TEYYQQLQCI
KRFKDIFNTP EQLDLPPQGY NPLLLQELFM APRRGGKLDP HWLGTLHESP ANVYSFVSNA
LIAVCRETDN ERLNDVALAC AELTASCNVL SEEWIYALQS LCSGSKSPRY PHLGGQVDIG
QLKTHNALAV FVCILVARHC FSLADFVSKF ALPTLARSVS AGGAELSVDA EAGARLTCHL
VLKLFKTLEI PQPGMYSVST SPNPLHAVGN DFSIRLSCDR HLLVGAHKTI PIAAVLAVLK
AILIVVDNAA LKTPLASGSG TSSGGLGGAF GSGKRSGFNT PVHPGSTPKS NEQRPADLSQ
ILGTSDLQLG SSLTSEPEAL QQPSVGGMEQ ISLLEFAQAV LKQICAQEHV LERCLKNAEQ
LCDMIIDEML TAKQAQRVLH MICYPEPEFN IISELDQRSM IVRILENLGQ WTLRISWLDL
QLMYRQSLSN NAELNVWLDT VARAAIDVFH MEEVVLPGAV KATHKPKPST WLVAPLIAKL
TPAVQGRILR VAGQVLESMN YFSKVSKSDC NSSGSGDERE KSNSCHSSNS YGLGGVPARN
KKMPLDYQPF LGLILTCLKG QDEYKENLLV SLYAQLSQCL QSFAELDTIG GIDEPQAREE
ILDALQLRFS LVGGMFEAIQ KNSTPTTDWA ILLAQLVCQG VVDLSCNREL FTTVVDMLAT
LVHSTLVSDD ERHYMNLMKK LKKEIGEKNN ASIRVIRQLL PLYKQPTEVI ACEHSGMDTK
GNKICDIDKK QLRISDKQRI SVWDILEGHK NPAPLSWVWF GAVKLERKPL TYEEAHRNLK
YHTHSLVKPS SYYYEPLPLP PEDIEPVPEK ICIKDEMKAD TPSSVDQSPS AVVGGTGRGR
GKGTTTRKRK PKNPKTPPVV NTQQQQPQLA QQPQQPQNVQ QQQLQQQQQQ QQHMQQQHMQ
QQQMQPNQMG QMPMNMPMNM QQFAPNPNNM MQQNAMLQQQ QQQQMQQMGN NPMQQQLNVG
GGNGQPNPQM NFMQQGPGGG GAGPQGMPGQ QQQWHNAPQQ QQPPQPYHNQ YAPHQQNMQS
NRIERPPLNA NSKQALSQML RQRQPFQQQA QQGPGGGFNP MQQQPQASQQ QPGPQQQMNP
NQMRQQQMNP QQNPQSVAAF NAMQQQPQQN AQQQQMNPNQ QQQQQFMRGG NMRPGMAPNQ
MNQMNMGGQG MSQNPMMQQQ IPQNMVGMVN PNANQMMQSG GAQGGNGVGV GVGVGVGGAG
NNPNMGMGGM PQQGMIQQQP QQQPQQQVQF QNFQNQYQQQ QQQGMQQQGG GAGVGVGVGM
APNQQQQQQA NMMGNFNPQM QQGNRNNPDF MAAAAVAQQQ QQQQQQQRVV PGGMMAGNRN
QYMNQAPNVT MSTMMGPGPG GVVGQVPPYA RQQSAGGGKP GVLNTQQQFQ QQQQQQQQLR
HQMMQLQGMG GGAGGGMGAG PQQGGGAVGG GAGGGMVPQQ QSMNQQQTPN LVAQLQRQNM
MGQQQYQPPP Y
