MED13_ARATH
ID MED13_ARATH Reviewed; 1921 AA.
AC F4I096; F4I095; Q9C8A3;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE AltName: Full=Protein GRAND CENTRAL;
DE AltName: Full=Protein MACCHI-BOU 2;
GN Name=MED13; Synonyms=GCT, MAB2, MED13_1; OrderedLocusNames=At1g55325;
GN ORFNames=F7A10.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-220 AND LYS-226, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [4]
RP FUNCTION, MUTAGENESIS OF SER-407, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20023166; DOI=10.1242/dev.043174;
RA Gillmor C.S., Park M.Y., Smith M.R., Pepitone R., Kerstetter R.A.,
RA Poethig R.S.;
RT "The MED12-MED13 module of Mediator regulates the timing of embryo
RT patterning in Arabidopsis.";
RL Development 137:113-122(2010).
RN [5]
RP FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX PubMed=21257604; DOI=10.1093/pcp/pcr013;
RA Ito J., Sono T., Tasaka M., Furutani M.;
RT "MACCHI-BOU 2 is required for early embryo patterning and cotyledon
RT organogenesis in Arabidopsis.";
RL Plant Cell Physiol. 52:539-552(2011).
RN [6]
RP IDENTIFICATION, SUBUNIT, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22247249; DOI=10.1093/pcp/pcs002;
RA Imura Y., Kobayashi Y., Yamamoto S., Furutani M., Tasaka M., Abe M.,
RA Araki T.;
RT "CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple
RT target steps in Arabidopsis.";
RL Plant Cell Physiol. 53:287-303(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors. Acts closely together with MAB12. Involved in
CC the regulation of embryo patterning and cotyledon organogenesis. May
CC act through transient repression of specific genes such as the ones
CC responsive to auxin. {ECO:0000269|PubMed:20023166,
CC ECO:0000269|PubMed:21257604, ECO:0000269|PubMed:22247249}.
CC -!- SUBUNIT: Component of the Mediator complex. Interacts with CYCC1-2
CC (CDK8 homolog). {ECO:0000269|PubMed:21257604,
CC ECO:0000269|PubMed:22021418}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=F4I096-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I096-2; Sequence=VSP_043977, VSP_043978, VSP_043979,
CC VSP_043980, VSP_043981, VSP_043982;
CC -!- TISSUE SPECIFICITY: Ubiquitous. Highest expression in the shoot apex.
CC {ECO:0000269|PubMed:20023166}.
CC -!- DEVELOPMENTAL STAGE: Expressed in both the apical and basal cell at the
CC one-cell embryo stage and in all cells of the embryo and suspensor
CC through the globular stage. Decreases in the periphery of the hypocotyl
CC and on the abaxial side of cotyledons from the heart stage to the
CC torpedo stage, and by the bent cotyledon stage, restricted to the
CC vascular tissue and the shoot and root apical meristems.
CC {ECO:0000269|PubMed:20023166}.
CC -!- DISRUPTION PHENOTYPE: Abnormal embryo and cotyledon development, and
CC embryo lethality in most cases. When viable, plants are small with
CC greatly delayed flowering time and sterile flowers.
CC {ECO:0000269|PubMed:20023166, ECO:0000269|PubMed:21257604,
CC ECO:0000269|PubMed:22247249}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51574.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g55325 has been split into 2 genes: At1g55320 and At1g55325.; Evidence={ECO:0000305};
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DR EMBL; AC027034; AAG51574.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33227.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33228.1; -; Genomic_DNA.
DR PIR; D96595; D96595.
DR RefSeq; NP_001185237.1; NM_001198308.1. [F4I096-2]
DR RefSeq; NP_849809.2; NM_179478.2. [F4I096-1]
DR AlphaFoldDB; F4I096; -.
DR BioGRID; 27203; 1.
DR STRING; 3702.AT1G55325.2; -.
DR iPTMnet; F4I096; -.
DR PRIDE; F4I096; -.
DR EnsemblPlants; AT1G55325.1; AT1G55325.1; AT1G55325. [F4I096-1]
DR EnsemblPlants; AT1G55325.2; AT1G55325.2; AT1G55325. [F4I096-2]
DR GeneID; 841978; -.
DR Gramene; AT1G55325.1; AT1G55325.1; AT1G55325. [F4I096-1]
DR Gramene; AT1G55325.2; AT1G55325.2; AT1G55325. [F4I096-2]
DR KEGG; ath:AT1G55325; -.
DR Araport; AT1G55325; -.
DR TAIR; locus:1005716738; AT1G55325.
DR eggNOG; KOG1175; Eukaryota.
DR InParanoid; F4I096; -.
DR OMA; LLVTDWC; -.
DR OrthoDB; 19529at2759; -.
DR PRO; PR:F4I096; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I096; baseline and differential.
DR Genevisible; F4I096; AT.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0040034; P:regulation of development, heterochronic; IMP:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0090213; P:regulation of radial pattern formation; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR021643; Mediator_Med13_N.
DR InterPro; IPR041285; MID_MedPIWI.
DR Pfam; PF11597; Med13_N; 1.
DR Pfam; PF18296; MID_MedPIWI; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Growth regulation;
KW Isopeptide bond; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..1921
FT /note="Mediator of RNA polymerase II transcription subunit
FT 13"
FT /id="PRO_0000415734"
FT REGION 400..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1485..1528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..431
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1512..1528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 220
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:17272265"
FT CROSSLNK 226
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:17272265"
FT VAR_SEQ 216..245
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043977"
FT VAR_SEQ 382
FT /note="R -> RFWLQNWIGPSLAGSSLFMHW (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043978"
FT VAR_SEQ 1037..1056
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043979"
FT VAR_SEQ 1152..1171
FT /note="GESTSLSFVKEVMLKHDRNW -> VYETCR (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043980"
FT VAR_SEQ 1559
FT /note="R -> RGELLDTHIFPFGGISSRQDTKGLQCLFVQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043981"
FT VAR_SEQ 1921
FT /note="V -> LKVLTTDSGSQSLSMSLPRDHLDGICFQHTDCNFLRCCSPFLSGHIR
FT RDGEQVNGQIKTAAIYYGVSLFPGKIDNLNAMVWSHSCCRPLSSMHHMA (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043982"
FT MUTAGEN 407
FT /note="S->N: In gct-3; uncoupled cell division, pattern
FT formation and morphogenesis during embryogenesis."
FT /evidence="ECO:0000269|PubMed:20023166"
SQ SEQUENCE 1921 AA; 208462 MW; 65425954D40C7C88 CRC64;
MWTNVFRIGG LHNVSWFQFL PSETELNPGF DRSSRAEQNE VATYLVLSSH LRLQKEGFLT
TWTNSFVGPW DPSQGLYNPD EKIKLWLFLP GRHSSISDKA QAAVSKLRVV ASGIWVAPGD
SEEISVAFSQ SLRNCIERAL SGISYMRFGD VFSKFSPQSE EYLRRGQPTV EFIFAATEEA
VFVHVIISAK NVRTLSSGDA ERMLRSSLKN SSYRLPAFRK CLGLAKSEDN RLCYINTSHR
PMLFPPVIVS PHGMRGSLTG FCPNDLVKQV YFSSGNLKTS TGYVGLPSHI GRGSRLINGN
HCYVEVTLGC CQNRNDNTSQ ANSTFAVNLP HNQCPEPSVG SKDHRKGQSD LSSVCEKKFI
YPAEAVLVPI LQSAFAKFSL KRAGDFDCLG ASENKSDGFY EKNGYNSSGS SRNSSISSTS
SASSGSGWRM TSRTGDLDAD ADSLTCRQSG LTCNDDRLKM GSKRPRTGMA ESFGQVGIEN
DQIGWDWDAD DDDDDREVGM DIKALLSEFG DFGDFFENDA LPFGEPPGTA ESHVLVFPPD
SADVGSSPVD MMDVSDQIVL PVGFSSFESF NPVPPIIDEC LIKSQEVLHS SITSVPSNQM
SISSTGEFDH LLKAEAMMTF APEYGAVEAP MSEISSTFFK SPYLPKSHKV ESSNSRTSNY
VYGATPPTTD SDGAGDMILF GSKSCIGNNA GRTLYHSREH YTQVEGRKGR HDKLPTVISD
NSSTKEGVSQ SIHSKHSAAN AVKVVQGKKT DGISAVVSTL LSSKTLLATD VGSVMFQAFM
CRMRHIITSS KHSSPVSLTR LSGNFFLNQL SNEPSTLTDN ISARNEIYKK DIPTRIAGDF
DGGMLDSHMS APVGVWRTVS VPKTAKPASS PNIEAGSSLP HSSFSEDSLL SYGQRQPLQD
LLDGIALLVQ QATSFVDLAL DSDCGDGPYG WLALEELWRR ELSCGPSAGH AGCGGTLASC
HSLDIAGVKL VDPLSAEVFP SSVITLLQSD IKTALKSAFG QSDGPLSVTD WCKGRNQSGD
GGSISEGFTA ESALSEVNGV NISDDFIIDK YFGKQAVSNA IDGGKGDETA QSQDIYSSEL
LRPTLFVLPS PAILVGYQDD WLKISTNSLT HWEKAPFEPY ALPKSINYAV VCPDIDPLTC
AATDFFQQLG TGESTSLSFV KEVMLKHDRN WLGTHLPQSL GNQMEKDVGR LSSSGFVLLD
CPQSMKIESN NTSLLGSLSD YFLSLSNGWN VNSYLKSLSK ALKGLKLGSG LYTNQKEGSG
SPCVVVYIVC PFPDPSAVLR TIVESSIALG SVIQSDRDRR SILNSQVARA FSSSTAVDEA
SISHIPVLSG FSVPKLVLQV VSVDSIFRIT SPSFNELVIL KDTAFSVYNK ARRISRGMPN
DAFFSSSLPS RSSSALTPMN SISGIWKDCG GSRMTGSTHP RDGEIDVSLR TSGWDTSTSW
QIPRSGGLSC DPNRNGDFYL NDEIFYLFEP LFILSEPGSV ERGVSPTFTS LGSESSKPIP
EDGGRGSGPG MNSMEGITSG SSSQGDVSQL EGKAIPSLHC CYGWTEDWRW LVSIWTDARV
LQQGCQILQA CSSPDNGSFK PRDFVITRIG NFFELEYQEW QKAIYSAGGP EIKKWPIQLR
RSAPSGIATN SNGSSLQQQD LSLIQERASS TSTLYSSHSK QSTFVKGSMG QSAGRKQIMG
GQTISGTPRG LFQWVHSISF ASISLDHSLH FVLPAELVSA GGGQSSTGMS SVNYIEGFTP
VKSLGSTAFS YMMIPSPNMR FLHPSPLQLP TCLTAESPPL AHLLHSKGYA IPLSTGFVVS
KAVPSMRKDS RINVKEEWPS VLSVSLIDYY GGYDNAHDKI LQGIVKQGGG TKETRDFEVE
SHLILESIAA ELHALSWMTV SPAYLDRRTA LPFHCDMVLR LRRLLHFADK EVSRIPDKTG
V
