MED13_CAEBR
ID MED13_CAEBR Reviewed; 2974 AA.
AC P0C657; A8WSM4;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE AltName: Full=Lethal protein 19;
DE AltName: Full=Mediator complex subunit 13;
GN Name=let-19; Synonyms=mdt-13; ORFNames=CBG03168;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC {ECO:0000305}.
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DR EMBL; HE601438; CAP23483.3; -; Genomic_DNA.
DR SMR; P0C657; -.
DR STRING; 6238.CBG03168; -.
DR PRIDE; P0C657; -.
DR WormBase; CBG03168; CBP42207; WBGene00026086; Cbr-let-19.
DR eggNOG; KOG3600; Eukaryota.
DR HOGENOM; CLU_226203_0_0_1; -.
DR InParanoid; P0C657; -.
DR OMA; WRFFDRK; -.
DR OrthoDB; 30695at2759; -.
DR Proteomes; UP000008549; Chromosome II.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR009401; Med13_C.
DR InterPro; IPR021643; Mediator_Med13_N.
DR InterPro; IPR041285; MID_MedPIWI.
DR Pfam; PF06333; Med13_C; 1.
DR Pfam; PF11597; Med13_N; 1.
DR Pfam; PF18296; MID_MedPIWI; 2.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..2974
FT /note="Mediator of RNA polymerase II transcription subunit
FT 13"
FT /id="PRO_0000314243"
FT REGION 284..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 340..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 624..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 677..749
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 764..801
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1032..1063
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1099..1134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1140..1159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1281..1342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1416..1486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2052..2078
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2247..2309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2347..2974
FT /note="Mediates transcriptional repression"
FT /evidence="ECO:0000250"
FT COILED 351..396
FT /evidence="ECO:0000255"
FT COILED 2220..2301
FT /evidence="ECO:0000255"
FT COMPBIAS 284..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 630..654
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 685..726
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1036..1050
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1140..1156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1287..1302
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1323..1342
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1430..1462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1471..1486
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2062..2078
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2247..2262
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2277..2294
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2974 AA; 337537 MW; ED55A4325CDC3646 CRC64;
MKATVEGEKD ASRKVKVPKR PEVNNGGSLE DCISNVFSLL ELPGIKWKCF RPKLNAPRGV
PLTSDLVLKA YSRCLTDGIL CTWRRKPSPP TGNNELLPPT HFFSNDSPKE LWVFWYDAEP
TALGKYCEGL DSDEELSSAN QMNIVSYEVR TILFKALHVV LERDLTKDGF VRFGRWFTMP
LVARDHYLHF MYPSHSPAIR FNFFVHGTST ICASIQAQRQ PTLIKLARRH FECKTPKRFP
VVIGPWSMRG YLIADQMTLL ADQKIQEAAE KEWNQWKEYL QLEEKEPENV TEERQKTPEA
EPPAPPQTTR VLLASSSDED QEKNGGELKS EEVSQKIRWM FEPDLRQDKP KEETAAEKEK
RMAAREVRRL RRQRREERRR EMEKQRRADD NLEDYDSDVV TDQDEDEAGK EEIVNNDVPK
MILIEIDNVR LLYPSKFVCI TVEEDRQMLE SMGFKCHPMP QELQVPGRRP RNKNVVNMSN
PLLSTMAVYQ YVQNEKRAER EHFYPRNKPV EIETDTNFEI LSNPKARRVA PPKSPTFLSW
ESQRANHFDT TDIFVNRLFE KLFRLDADTF DENPRLIKFA CLSSQFIRRK WKWLHKKQIL
FCGKARKRFG IGSPQNFALN MMKSREKRKE YQPYHRKRPA KSVKEKKKKA KRKVKKFVRY
NSPEQFLASM SRTRKKFNAW KQKKKGPPPK KDLAKKEAAA DKDKDKDKEK DKEKDKDNDD
DPFVMDKVSQ LDWANFSEDE TNGNSFADIE EALVSEEHIK FSSLYNPAGG DIEEGDETSN
ESPDEKTPEG SPNGSPRPKE EIEPFFPITY LAAKPSNIES DLGPDVDDMT EEQFNTEYVP
KEYDITQDPK KVKNGQIPIS EFSKDNQFYH GEICRLGLRG YKKICAERDR EEAARRTKWL
QLYRLKVEKR SIKTARQCHR DYLRQIAKRC LRKALRRLRI TAKKDALAPP PPKPKPVDPV
LKKKEDAKKF EMSLKIMERD LGNLDMSAFC QVREKAGPHG VSFPEIILLT NPKWLKVRST
AGYMKPNYFN SPHGSFDHDS EPEFDEQRTG LGEGTSDQYQ DGESVDMIDI ERELANDPSS
YLSNAEHIRT LGEHIGPDGM LSPPASNEMP KGGPLSVGPA SIESQGLNQI YPTPPSVQML
QADASQAHSP SIGGKFRSTA NDDIDRAAAS GKTIVDIEIE DIETGNMQRW NKIANQSKLS
KFLVASRDNP ILKGRKTKYD TPATDKFEIR CQVERVTVPM AYESMVTRNF ECAPPHTFAE
LIRQSRTGIK KYTRPLQIQS TLARPPPGAT SPLPPPTPMF NPHTLPLHHT SPFSAGPMSH
HSMGPPAYPP TPGPYPPTTP TYPGMTPRPG SSYGPGYPNH HQMMPPGYPN QMGQMGGMNG
MGGMPGMGPI GMQRQFSNPQ MYQHHQMMRM QQMRMQGGLP GYGAPGVPQY PQQSPVSGGP
ESIRNTDAPN DPTVSKLQSA VSRNGKSDDE SDAATSIPTA TDDATAATTT AAQLQLQRSQ
AHQIQHQPNQ QTMQSLQLTP EQLQIQQSQQ VQAVFQQLPD REKRKYQRRQ IQLMNGLMPT
SLQPASIKAL KNPPDANIPY KRQDIIYNPP NPAHPRGDSL TIAIVLSDTL LDLHFDSVFD
ACPICSCSVS IRSRDLGMYI IPHAVLSSRE IGETNKREYT TGTWSGFHVN SATSCTCGFS
AIRHRYLSCC AGLFDEDADE ATASEHANAP VIPPLFARNT TKTRDMMWFD PQSVHDLALT
DQIRQMAFSN SLGKAVSQMA TEKEHRRNIT NAVDIGTDIT VPTEYVLSHV DTLELMMLGM
SALGPMQTPE TTGNQFTPQS KYLSYFHPWG FQTANEIAEL ESSEWVDLLD IITPTLESSM
KQARRPSVET PFVIEGPMTW KQVVTKAIRG KPPTDEDEDF SLAEPVPAVM RALEQEAVRA
APNIEQYYDQ AELGPVDQPK DVMYITIIPD NDDIYERTVK FMHTMTETYE RMRLGRHIPF
PVSTGTASRF REVLKTYQHQ FPYQEQNQQL VEFTNNRLEA VPQELSNNPD PLNTDPEKWS
KRLKRKLELS EELKKADEHP APPSTQSENS EGNAATPAAE TLSSIFSDDA FVEERHINST
EEAPTTTERP PNSLPYRITN WHEKDDDTYE HPPEVHDTLP EGFYEREGIL RVGKPLEPLR
ISHTVVSTRE FDNMTQHLSD AQGFVSKLRI FLQQMEDLVF HTLSGSSEAF ERRGYRYQMA
VEGRLKRQKH KRDIEEERLR YEAAKVQDEA DKREKMDEAD LFPGAEVGEE TPSPWVDDEL
EEKKRNKQKE NEQYPAEESQ APSPVPAGMI HIPETLSEQE RKVQPTLADM FADPSTVAPI
NAQNIPWKQR DTRVQNPFPY SNQPPVAFEA VGSNDTDAYS TLPHVIVLYV VNPFSYGAEG
QSALHMRIAL LSFIRAYNSI VGRLPFTKRT QLQLEIIGME SLDDMVKGIP DYLNDSQVPF
DILHDYPVRN ERPSESGQEA AARSLSVAVY THPRVFTPEV YKAVSARCMT AFGPGSQLIN
TVNNIEKINQ EAFYQMSKRS KTALNNMDGY MGMMGSHQVE TKAHISYRVP SNIVCLAPPP
AIYQMDEEGK PILNQLDEQT LFISYCLVGT EYLVATATDS QGKLIDNCIA NMKPRRLHNQ
VYRYKNKTQI LDGMGKLWSF ILGVMSSDIK NWRLVVGRLG RIGHGEFRAW THLLNKTSLL
RYSSSLKDIC GACRSMPSAI GTPAILSACL ITLEPEPSIR IMPKFHDIDE SIKKNLIFQT
PGDLSCTHIL TFPVGTEINL EVQDQTADTK GDENWEFGDL DIMEGLDDGD TEIMKDLGLE
TPSSAAIRQT GGVSMFFSED SSSIEIQNQP LASGYYISTA PAPELPSWFW ATCPSAKRHS
PVHLKSSLHI NISEVKNDDI AMESAKDKEK EKEEKDIHPL ESRQTEEVLR HVLESYNALS
WLNLNRQTGD RYSCLPIHVQ HLLRLYHSVA RLLV
