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MED13_DICDI
ID   MED13_DICDI             Reviewed;        2678 AA.
AC   Q54S20; Q9NDS4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE   AltName: Full=Aggregation minus B protein;
DE   AltName: Full=Mediator complex subunit 13;
GN   Name=amiB; Synonyms=med13; ORFNames=DDB_G0282375;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=AX2;
RX   PubMed=10651904; DOI=10.1046/j.1365-2443.2000.00305.x;
RA   Kon T., Adachi H., Sutoh K.;
RT   "amiB, a novel gene required for the growth/differentiation transition in
RT   Dictyostelium.";
RL   Genes Cells 5:43-55(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=AX2;
RX   PubMed=15259052; DOI=10.1002/cm.20015;
RA   Asano Y., Mizuno T., Kon T., Nagasaki A., Sutoh K., Uyeda T.Q.;
RT   "Keratocyte-like locomotion in amiB-null Dictyostelium cells.";
RL   Cell Motil. Cytoskeleton 59:17-27(2004).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). Required for the
CC       starvation-induced activation of the ACA (adenylyl cyclase) expression
CC       pathway at the growth/differentiation transition. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: The level of mRNA increases 4 hours after starting
CC       starvation and remains relatively constant thereafter.
CC       {ECO:0000269|PubMed:10651904}.
CC   -!- DISRUPTION PHENOTYPE: When starved, cells fail to turn off expression
CC       of the growth-phase gene cprD and to turn on expression of the adenylyl
CC       cyclase gene, resulting in an undetectable level of cAMP and a strong
CC       reduction of the cAMP-relay response. They join streams of aggregating
CC       cells but with less efficiency than wild-type cells and show a
CC       dramatically reduced rate of sporulation. In the migratory slug, they
CC       are almost exclusively located in the posterior region and many cells
CC       are found in the slime trail that is left behind the slug. When the
CC       fruiting body is formed, most cells are found in the basal disks while
CC       a minor part is located in the mature spores and stalks. Starved mutant
CC       cells are elongated laterally and form one large laemllipodium along
CC       the front side arc of the cell. They have a keratocyte-like behavior,
CC       moving persistently without changing directions over long distances.
CC       {ECO:0000269|PubMed:10651904, ECO:0000269|PubMed:15259052}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC       {ECO:0000305}.
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DR   EMBL; AB030033; BAB01489.1; -; Genomic_DNA.
DR   EMBL; AAFI02000047; EAL66047.1; -; Genomic_DNA.
DR   RefSeq; XP_640219.1; XM_635127.1.
DR   AlphaFoldDB; Q54S20; -.
DR   STRING; 44689.DDB0214900; -.
DR   PaxDb; Q54S20; -.
DR   EnsemblProtists; EAL66047; EAL66047; DDB_G0282375.
DR   GeneID; 8623759; -.
DR   KEGG; ddi:DDB_G0282375; -.
DR   dictyBase; DDB_G0282375; amiB.
DR   eggNOG; KOG3600; Eukaryota.
DR   HOGENOM; CLU_227451_0_0_1; -.
DR   InParanoid; Q54S20; -.
DR   OMA; NKCRRIS; -.
DR   PRO; PR:Q54S20; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR   GO; GO:0006935; P:chemotaxis; IMP:dictyBase.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:dictyBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR009401; Med13_C.
DR   InterPro; IPR041285; MID_MedPIWI.
DR   Pfam; PF06333; Med13_C; 1.
DR   Pfam; PF18296; MID_MedPIWI; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..2678
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   13"
FT                   /id="PRO_0000327756"
FT   REGION          1..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          140..285
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          495..524
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          553..615
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          852..993
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1070..1253
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1278..1394
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1462..1502
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1806..1838
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2075..2105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2225..2307
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2656..2678
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        852..873
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        923..993
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1073..1092
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1103..1207
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1208..1235
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1278..1350
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1358..1373
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1374..1394
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1806..1830
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        1993
FT                   /note="A -> R (in Ref. 1; BAB01489)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   2678 AA;  298838 MW;  81CD1A57137AC706 CRC64;
     MMGTKVPSYN NKQQSGSNAG GNVNNNNNGP NINNPNNPNN TNSLPTTSGY NSSRFSVPNL
     DTDNFMTNII SMGVNKVGWA LYRSNSNNNN NNINNNSSSS SSSNTSNNIN SNTLVNDPIL
     LAYLKTIELG VPCLWRKTRS KKPINNSSNN SNITSSTSTD SSLKNRLKNS PSETTPPNTT
     NNNSNNVTKD SPPNATNKMS TSPKSLSPTI SNNNNNTTAA ATTTTTTTNN SSNSPTSPTN
     NNNNNNNNNS PSSHNVVNSP PSTSSRSPPT VASVTSNTST IQPISPIINR QTSSHNYQQP
     QQPYYNHQPL QPIQEYITTY ELWVFNISNT SLLDFSHLNN VESGIVNLDN IGCNNSVIVQ
     YLFKSLYNLV EYNMVSNDFI KIGNSFIKSS HILQKQKQSR NDYNNSIISG SVGSKKRLFE
     DFTNLYIEDD CLLSCSFSFF IYNKSIHLHF NVDKKRLKPL QELMKSEYIN SPLFTSPNAF
     IAFFNNNNKN NINNNSNNII NNNNNSNINN NINNNNNQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQQQQQQQQ QQQQQQTINN NSNNISSNND NNNTPSQMVP SIDGHDSSSS SSTPSQVTMQ
     TTPTQEFSNN NNNNISINTT TVPITATTAT ATNKITTAAT TTTTINNNNN KIQTESFGST
     RNKKLNDEEE INFSLSSQQW KQLFNIGLAN KAIIPKQQTP QQQEQQQIIG TIFKKNQLEV
     PKSIKTIITR GNSLPLINTF FPIELLFKFF PPISDISQYT YHEKLKEFGL EIFDKSPYAL
     LEKGLNLFIN NNNINIENNN NTSNEINNTG TQIPTKYKYV DFWSLPLFHS KESLIYGNDH
     FNATYCVKAV PSPSSPLTQH PSSPHSPFNN VNSPKSPQHP PSPKTPMISD DDQSLTANNE
     KKRHGKSQKK GRSSKRKKDD NSNNNNNNNT TTTSTITATT TTTTPTAATT GNDNFLAPQS
     KPPPSNIQET SQPQSLTTVQ PQPPPQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQQVVSSFS GQGLNSSMDL FGSDAFDTFK NKEDDLMHLS DTDIDLDLWT SHYVPPNQPT
     QNGSQKNNQR PLPVEDPFQV FQVPNKPATT TTTTTTTTTP TPNPTTTTTQ PQTQPQQQSQ
     QQQQPQQTNP ILPTNSNLIT NQKPQQYQPP LQDPFQSIDS QQPKSIQSPT LTNQPIGMVS
     PTLTNQPLQQ YQPPPPPTQQ PQQAHLKPPP PKQPSAKKNS KDGINSDENK PFLDGQFLCP
     VPYKAPSLSQ NQLPILHLPH NTEQSPSNDD LSNPNHLHHG TPTSAISGVG GSSSSSGNNM
     IGSGGIVGSG GGNTNVSGSG GGMVSDPSKL TYSSEHHDPH HHHHHHHHHH PQNSQNLRNS
     YGNSNGVGGS QDESIDGLTT VYRNLTLSRP LGDNKNKNNA PYYCAPSYKP LDEHSLPHQL
     KFEYSYTPLV KPQNQLNQTK YSPFSNSHHN HHHNTTTNNN NNHNNNNNNN HPNNHHQNNN
     HHHLNITSSL FQPSNIQNVN SLPNIGSQKI MSISPPITPP MKGGHMIGTD INKNNNKKNN
     DEDENLEILS TIKQKDVNGL DSNLSSSALL SFFDQAINIK TTPPRSPVLS GYSGGGSNMN
     NGINGNSGFI GNGNSGFNTI SSYQQNNISI EESLDLAFTI QQMTITNYDS TFGFSIDNSP
     LIKQSIDYYL ENWLKIYTET GKVFNHHSLQ FLTEESISDA LDTVIDPLTT NTILETTVKT
     LFTFSNEIFK KIFPLSSFPL SIKEFCHLPS ITLNNINNNI DLLNSQTYQN TNNSSSGGGI
     ESQQYFSGSS GLNNSNDRNS MIPMDNSDDT SDLMSMDDHN SKNLITGTSL ISTTASGAGA
     AITTNLINQN YEEFEWLPIP KIIVGYKEDW LEVPISTIKF WDKAQLEPYS PKKNITYFVL
     CPDTTAMRMH VPAFFSDLSC IYETNHLGNH VTQQQSQVQQ AAAAAVAASQ PTQQSSQHQS
     QQQQQQAAQQ AAAAAAAAAA AQKNSSPFYN GIYYIPSAAN TEFNPQTFIN SYLLTCIQFG
     DYLKELSTKY SENNCMVLYI VNPFSDLSQI SLSNQNQNQN QNSNSNESQQ QQQQQSNNKQ
     KQTQTNEDIN HIRNSYLFMS SCFQTIVRDI TCNQSLSITI QTIPIEQILF DDHPSIPVSK
     ELAFSVFNKC RRISTISPMN FSINMNNINN MIGNNNSNSN NSSGWYYQLF NKIYEPLFIL
     SNESSSSSSS TISTSTSAST STSTSNTTTK NQQQQSQSQQ SQQQSQQPKQ LSTPTTPTTN
     SVSNSPSLQP QPLQTPNQLQ PHQQEQKQQQ QIPIIHFSYM LSDDNKWISC TIVDEQGELL
     ENKLIPLAYS QIIGDYIWKD SFQNAWFFLT EIIGLSLSGQ PCQVVIGKFG IISMQEYNDW
     KTTIIHFEDN LEVIKQHQQQ SQSQNYSSTI DQIYKIENVI FLNLTLNNNI KSFLTEYLND
     SKLSNSNNIK SVSPLSGQSF AIYPESPIMY TYNFPMNDNN DDYNSILNNN NNNILPPLST
     SFVITSPSNL SGSVLTPTTT NSNVINNNQS KKEWPFTYML SIVGIQSLQH QQQQQQQQQQ
     QQQQQHPNII NHQELIRSIT RSYHRLSYLN ISPRYPDRLS ILPIHFSISK RMSRTVCLIS
     PLNPIPPPIL FNRCPKLTPN SKQSPSPINS PHLNSNNT
 
 
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