ID   MED14_AEDAE             Reviewed;        1578 AA.
AC   Q16U49;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=MED14; ORFNames=AAEL010020;
OS   Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Aedini; Aedes; Stegomyia.
OX   NCBI_TaxID=7159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LVPib12;
RX   PubMed=17510324; DOI=10.1126/science.1138878;
RA   Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA   Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA   Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA   Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA   Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA   Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA   El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA   Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA   Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA   Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA   Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA   Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA   Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA   Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA   Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA   Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA   Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT   "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL   Science 316:1718-1723(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAT38036.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CH477632; EAT38036.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001660571.1; XM_001660521.1.
DR   AlphaFoldDB; Q16U49; -.
DR   SMR; Q16U49; -.
DR   STRING; 7159.AAEL010020-PA; -.
DR   PRIDE; Q16U49; -.
DR   VEuPathDB; VectorBase:AAEL014851; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_001928_0_0_1; -.
DR   InParanoid; Q16U49; -.
DR   OMA; TEGEDNP; -.
DR   Proteomes; UP000008820; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1578
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304586"
FT   REGION          561..582
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1009..1173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1510..1578
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           49..53
FT                   /note="LXXLL motif 1"
FT   MOTIF           739..743
FT                   /note="LXXLL motif 2"
FT   COMPBIAS        561..580
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1073..1093
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1094..1126
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1132..1151
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1529..1553
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1578 AA;  175327 MW;  F37F6368C0E710DB CRC64;
     MTPQPLEQGG AVASFIPTGQ EMAQRQNLIP LGRLIDFIIQ RTYHELTVLA ELLPRKTDME
     RKIEIYNFSA STRQLFIRLL ALVKWANSAS KVDKSAKIMA FLDKQSLLFM DTADMLSRMA
     RETLVNARLP NFHIPAAVEI LTTGTYSRLP SIIRERIVPP DPITTLEKRQ TLQRLNQVIQ
     HRLVTGNLLP QLRKFKIENG RVTFKVDHEF EVSLTVMGDG PNIPWRLLDI DILVEDKETG
     DGKALVHTLQ VNYIHQLIQG RIVDSPDALA EVYNCLHYFC QSLQLEVLYT QTLRLMRDRL
     DDHIHVDEYI PGTKLTVSYW RELTNKDPKS ELGYRLMIQT DQSDTTKQLA ILHLPSIGTK
     EVDIADRAVR SELLSMERLL VHTVYVRSLA RLGDLKTELQ LFLKDVDYSI QGTPAMLLVP
     VLNPCLRAEH IYITVDTHTG MLRCHVPKHL DCPIMPELQH ALNNDRSKLQ HLFSELRYWI
     TQRRCEKTLQ HLPATTQERL PLVFPADHPI EKIGPHRVYI QMCRHANVIL IVELKEKESS
     PNEMTYTFYL VLVKPSSMED SQSVTAGGTS QSSAPSAATT ESDGMPKMYL KVLSLIEFDT
     FVATHGPGTY VDEQTVGSKR KLLTGEGPSA KQSKTIYPAY FIPELAHVVA MCDEKLPFVA
     LAKDLTRRKI PHGGVQVEAN ATSLVLKLLT LPQPKPPVIP VSSSAAAAAA AQSGPDQKPP
     EPKVVSVPKI DKDVWNALLK RLLSVSVRAQ INKSIQTRMW TVELVFYGTP LASAHHKEQG
     MRRAVYLTYD MLPVDSVDKF VDMILNDWSK IVYLYTLVHD FREQIKNDKY NLHNIVTIKS
     YSYTSLLLAY GPNKEVNVNI YWDTEAKEFK MVFTGGNNAI NAHSMMRDQL QAHLNHNYDL
     AQIVYLLHET YQPLSSIAKL PIIPHLAILQ SPKIPVLSFC IIPQSPTLLR ISFQGVYCLE
     VRLRGSGLCS IRDGAYSRFD RSHVVEEFTP TQGLKGFLSK YVDETAVYRR RSQSEDDNPP
     SPITMEDPHG GPASVGSTFL SGGAMRGPQS PRDPGLRFAA PLTPPSGSNP HTPASPHPQG
     IGGSQSHPNF NMTSPPAPHM PHPSPSGGLM PSSPLNPQPS PMAAHSPGPT NLPYMSGHTD
     SPFSAHSPAA SNWPGSPGMP RPSPRPGQSP DHKAQKRLLI ISLKFTAPHH NTSRVLPARS
     WAGAIPTLLT HEALDTLCRA TIHPQKEIPG PELSPLERFL GSVFMRRQLQ RIIHQEDNLT
     AITSNEPGVV LFKADNLQYQ VFLNPNHMQS LHMKVSQAPM APTMDGKPPY QWPPEDLQIL
     EQFFDQRVAA PPYRPAVVTS FTRMLNLPSQ VMKDFIQIMR LDLIPELGQG NKWNVQFILR
     MPPSATPIVP VGTTTILSHR QKILFFIQIT RVPYLPNMEL KDSVTMLLPM VYDMTVNHTQ
     LAERREQIIP QLTSAVSAHL RRFVECTVLQ PGECSLFPAV RDLLMNLTLP NEQPPPGQMG
     NQIGTMGGMA PGGPGGPGPM GGQIAPSPVG GQVVSSPNPM MHSPMQMGGG GQQSNYGGMV
     GGGAQSGVPG GPGAGGPN