ID   MED14_ANOGA             Reviewed;        1639 AA.
AC   Q7Q6S8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 5.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=MED14; ORFNames=AGAP005700;
OS   Anopheles gambiae (African malaria mosquito).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Anophelinae; Anopheles.
OX   NCBI_TaxID=7165;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PEST;
RX   PubMed=12364791; DOI=10.1126/science.1076181;
RA   Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA   Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA   Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA   Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA   Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA   Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA   Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA   Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA   Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA   Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA   Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA   Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA   McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA   O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA   Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA   Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA   Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA   Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA   Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA   Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA   Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA   Collins F.H., Hoffman S.L.;
RT   "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL   Science 298:129-149(2002).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; AAAB01008960; EAA10746.4; -; Genomic_DNA.
DR   RefSeq; XP_315707.4; XM_315707.4.
DR   AlphaFoldDB; Q7Q6S8; -.
DR   SMR; Q7Q6S8; -.
DR   STRING; 7165.AGAP005700-PA; -.
DR   PaxDb; Q7Q6S8; -.
DR   GeneID; 1276369; -.
DR   KEGG; aga:AgaP_AGAP005700; -.
DR   CTD; 1276369; -.
DR   VEuPathDB; VectorBase:AGAP005700; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_001928_0_0_1; -.
DR   InParanoid; Q7Q6S8; -.
DR   OMA; SPAGSFM; -.
DR   OrthoDB; 1215335at2759; -.
DR   PhylomeDB; Q7Q6S8; -.
DR   Proteomes; UP000007062; Chromosome 2L.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1639
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304587"
FT   REGION          561..586
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          709..755
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1039..1243
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1558..1639
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           49..53
FT                   /note="LXXLL motif 1"
FT   MOTIF           768..772
FT                   /note="LXXLL motif 2"
FT   COMPBIAS        722..748
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1137..1158
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1172..1204
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1584..1599
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1639 AA;  180128 MW;  CF330C2A4CAE8E78 CRC64;
     MAPQPLEQGG AVTSFIPTGQ EMAQRQNLIP LGRLIDFIIQ RTYHELTVLA ELLPRKTDMD
     RKIEIYNFSA STRQLFIRLL ALVKWANSAS KVDKSAKIMG FLDKQSMLFI DTADMLSRVA
     RETLVHARLP NFHIPAAVEI LTTGSYSRLP SVIRDRIVPP DPITPAEKRQ TLQRLNQVIQ
     HRLVTGSLLP QLRKFRIENG RVTFKVDHEF EVSLTVMGDA PTVPWRLLDI DFLVEDKETG
     DGKALVHPLQ VNYIHQLIQG RIVDCTDALA EVYTCLHYFC QSLQLEVLYT QTLRLIRDRL
     DDHIHVDEYV VGSRLTVSYW RELTNKDPKS ELGYRLTIQT DPNDAAKQLA ILHVPSIGNK
     EADIADRAVR SDLLSMERLL VHTVYVRSLA RLNDVKTELQ LFLKDVEYNI QGTPAMLTVP
     VLNPCLRAEH IYITVDTHTG MLRCHVPKHL DCPIMPEMQH ALNNDWSKLQ HLISELRYWI
     TQRRCEKTLQ HLPAATQDRL PLIYSHTHPI ARMGPHKVFI QLYRHANVIL IVELKEKKTC
     PNEMTYTFYL VLVKPSSVEE GQSPDVLSGQ PPQPSAAGGP APGSDSANAA MPKMYLRVLS
     MIEFDTFVAT HGPGTYIDDP SPTSTSGTSV KRKVSPLDNA LSAIGPPLKQ QKTIYPAYFI
     PELAHVVAMC DEKLPFVTLA KEFSMRKIPH GGLQVEANAT SLVLKLLTLP QPKPPQAPPT
     PQQQQQQQQQ QQQPGTSDAK SSGAGASANE PKTVHVPPID KQVWNALLKR LLSVSVRAQV
     NKSNQTRLWT MELVFYGSPL PSLHHKEQGM RRAVYLQYEM QPVESVSKVV DQLLSDWSKI
     VYLYTLVHEF REQYNNEKYN LPSMVAIKSY SYTNLLLAYG PNKDVSVNIC WDTEAKEFRL
     VFTGGNSAIN AHSMMRDQLQ AHLNHNYSLA QVVHMLHETY QPLSSIAKLP IIPHLAILQS
     PKIPVLSFCI IPQSPTLLRI SFQGVYCLEV RFRGGGLCTI RDGAYSRFDR SHVVEEFTPT
     QGLKGFLSKY VDETAVFRRR SQSEDDNPPS PVTLEDPSAG AGNNGGGGGG GGGAGGGANT
     FLSGGTGMRG PQSPRDPGLR FAAPLTPPTS SNPHTPASPH PIGGGGGAGG AGGQGGQGGQ
     GGQQQSHMNN FNMTSPPASH MPHPSPGGGL MPSSPLNAQP SPMAAHSPGP SSLSYMQSHT
     DGSPFAALSP AASNWPGSPG MPRPSPRPGQ SPEHKVQTSH HYTSRVLPAR SWAGAIPTTL
     TYEALDTLCR ATPHPQKEVP GPELSPLERF LGSVFMRRQL QRIIHQEESL MAITSNEPGV
     VVFKADCLQY QVFLNPNHMQ SLHLKVDQLP MGPMMDGKPP YQWAAQDLQI LEQFFDHRVA
     APPYRPAVMT SFTRMLNLPA KVLKDFIQIM RLDLMPELVQ GNKWNVQFVL RVPPSATPIV
     PVGTTTILSH RQKILFFIQI TRVPYLPNME WKDAVTMLLP MVYDMNMNHT TLAERREPMP
     PQLTSAVSAH LRRFSECSVL LPDECSLFPA VHDLLLTLTL PNEPPAPGQM QMQLGGVMQP
     GGGPGVPGGP GGPMGGQIGG PTPQVVPQVG SSPSPMMHSP MQQMGGGGPQ PGAYGGMVGG
     PGGGPQSGGP VGGGPGGPN