ID   MED14_ARATH             Reviewed;        1703 AA.
AC   Q9SR02;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Protein STRUWWELPETER;
DE            Short=AtSWP;
GN   Name=MED14; Synonyms=MED14_1, SWP; OrderedLocusNames=At3g04740;
GN   ORFNames=F7O18.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Flower;
RX   PubMed=12426376; DOI=10.1093/emboj/cdf614;
RA   Autran D., Jonak C., Belcram K., Beemster G.T., Kronenberger J.,
RA   Grandjean O., Inze D., Traas J.;
RT   "Cell numbers and leaf development in Arabidopsis: a functional analysis of
RT   the STRUWWELPETER gene.";
RL   EMBO J. 21:6036-6049(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX, AND NOMENCLATURE.
RX   PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA   Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT   "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT   as the Med25 subunit.";
RL   Mol. Cell 26:717-729(2007).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH HDA19; LUG AND CDKE-1.
RX   PubMed=17526732; DOI=10.1128/mcb.01912-06;
RA   Gonzalez D., Bowen A.J., Carroll T.S., Conlan R.S.;
RT   "The transcription corepressor LEUNIG interacts with the histone
RT   deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to
RT   repress transcription.";
RL   Mol. Cell. Biol. 27:5306-5315(2007).
RN   [6]
RP   IDENTIFICATION, AND NOMENCLATURE.
RX   PubMed=22021418; DOI=10.1104/pp.111.188300;
RA   Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT   "The Mediator complex in plants: structure, phylogeny, and expression
RT   profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT   (rice) during reproduction and abiotic stress.";
RL   Plant Physiol. 157:1609-1627(2011).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. The Mediator complex, having a compact
CC       conformation in its free form, is recruited to promoters by direct
CC       interactions with regulatory proteins and serves for the assembly of a
CC       functional preinitiation complex with RNA polymerase II and the general
CC       transcription factors. Involved in defining the duration of cell
CC       proliferation. {ECO:0000269|PubMed:12426376,
CC       ECO:0000269|PubMed:17526732}.
CC   -!- SUBUNIT: Component of the Mediator complex. Interacts with CDKE-1,
CC       HDA19 and LUG. {ECO:0000269|PubMed:17526732,
CC       ECO:0000269|PubMed:17560376}.
CC   -!- INTERACTION:
CC       Q9SR02; F4IXJ7: MED6; NbExp=3; IntAct=EBI-1386248, EBI-1386187;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, stems, developing embryos,
CC       young leaf primordia, shoot apical meristems, inflorescence meristems,
CC       tapetum in anthers, ovules and floral organ primordia, but not in
CC       mature organs. {ECO:0000269|PubMed:12426376}.
CC   -!- DEVELOPMENTAL STAGE: During embryogenesis, detected in all cells from
CC       the early octant to the torpedo stage. {ECO:0000269|PubMed:12426376}.
CC   -!- DISRUPTION PHENOTYPE: Dwarf plants with disorganized shoot apical
CC       meristem, stem fasciation, abnormal floral structure, delayed flowering
CC       and sterile flowers. Reduced cell numbers in aerial organs.
CC       {ECO:0000269|PubMed:12426376}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ511271; CAD53582.1; -; mRNA.
DR   EMBL; AC011437; AAF04900.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE74130.1; -; Genomic_DNA.
DR   EMBL; AF466359; AAM09647.1; -; mRNA.
DR   RefSeq; NP_187125.1; NM_111346.4.
DR   AlphaFoldDB; Q9SR02; -.
DR   BioGRID; 4969; 12.
DR   IntAct; Q9SR02; 3.
DR   STRING; 3702.AT3G04740.1; -.
DR   iPTMnet; Q9SR02; -.
DR   PaxDb; Q9SR02; -.
DR   PRIDE; Q9SR02; -.
DR   ProteomicsDB; 238251; -.
DR   EnsemblPlants; AT3G04740.1; AT3G04740.1; AT3G04740.
DR   GeneID; 819634; -.
DR   Gramene; AT3G04740.1; AT3G04740.1; AT3G04740.
DR   KEGG; ath:AT3G04740; -.
DR   Araport; AT3G04740; -.
DR   TAIR; locus:2084948; AT3G04740.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_002817_0_0_1; -.
DR   InParanoid; Q9SR02; -.
DR   OMA; GSCPFIK; -.
DR   OrthoDB; 30000at2759; -.
DR   PhylomeDB; Q9SR02; -.
DR   PRO; PR:Q9SR02; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9SR02; baseline and differential.
DR   Genevisible; Q9SR02; AT.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0003712; F:transcription coregulator activity; IDA:TAIR.
DR   GO; GO:0009738; P:abscisic acid-activated signaling pathway; IDA:TAIR.
DR   GO; GO:0009631; P:cold acclimation; IMP:TAIR.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:TAIR.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:TAIR.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0009627; P:systemic acquired resistance; IEP:TAIR.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; Growth regulation; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..1703
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000418118"
FT   REGION          755..781
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1703 AA;  185509 MW;  5C68D7E02FB66A39 CRC64;
     MAELGQQTVD FSALVGRAAE ESFLSFKELV DKSKSTELSD TEKKVSLLKY VAKTQQRMLR
     LNALAKWCKQ VPLINYFQDL GSTLSAHDIC FTQAADSLFF MHEGLQQARA PVYDVPSAVE
     ILLTGSYQRL PKCLDDVGMQ SSLDEHQQKP ALRKLEVLVR SKLLEITLPK EITEVKISKG
     TVTLSVDGEF KVLVTLGYRG HLSMWRILHL DLLVGERSGP IKLEVTRRHI LGDDLERRMS
     VAENPFTILY AVLHELCVAI VMDTVIRQVR ALLQGRWKDA IRFDLISDTG TTPANQEGEA
     DSVSLRTPGM KLFYWSDSDK NSGPFIKIEP GSDLQIKCSH STFVIDPLTG KEAEFSLDQS
     CIDVEKLLLK AICCNRYTRL LEIQKELLRN TRICRTPSDV ILQALLDEPG IEGDNMVDSK
     ERVEPEVLRV RAYGSSFFTL GINIRTGRFL LQSSKSILTS SILEEFEDAL NQGSISAVDA
     FINLRSKSIL HFFAAIGKFL GLEVYEHGFG INKVPKSLLD GSSILTLGFP DCESSHLLLM
     ELEKDFTPLF KLLETQMDGS GKPQSFNDPS NILRAKKIDI GQIRILEDDL NLITSDVVKF
     VSSFSDAEGI NQASGHRQPG LVDEALTEMS GSQLSFSSVV DGVFGLQKVT SALMSIDGHG
     LVPKNLSAVT GHGKAPMLTS YHSDSLYNRQ GPLQSSSYNM LSSPPGKGSA MKKIAISNSD
     QELSLILSPS LSTGNGVSES GSRLVTESSL SPLPLSQTAD LATSSAGPLL RKDQKPRKRS
     ASDLLRLIPS LQVVEGVASP NKRRKTSELV QSELVKSWSP ASQTLSTAVS TSTKTIGCSY
     GNLIAEANKG NAPSSVFVYA LLHVVRHSSL SIKHAKLTSQ MEALDIQYVE EMGLRDAFSD
     IWFRLPFAQN DSWQHICLQL GRPGSMCWDV KINDQHFRDL WELQKGSKTT PWGSGVHIAN
     SSDVDSHIRY DPEGVVLSYQ SVEADSIKKL VADIQRLSNA RMFSLGMRKL LGIKPDEKTE
     ECSANSTMKG STGGKGSGEP VDRWRAFKIE AVGLTSLWFS FGSGVLARFV VEWESGKDGC
     TMHVSPDQLW PHTKFLEDFI NGAEVESLLD CIRLTAGPLH ALAAATRPAR ASTATGMPVV
     PATASSRQSN QIQQTQGIIA PSTLAAPNAT GQSASATSGN TVASSAPSPL GGGFHGVAML
     AAAGRSGPGI VPSSLLPIDV SVVLRGPYWI RIIYRKRFAV DMRCFAGDQV WLQPATPPKG
     GASIGGSLPC PQFRPFIMEH VAQELNGLEP NLTGSQGATN PNSGNPTVNG VNRVNFSPSS
     ARAAMNRVAS VASGSLVVSS GLPVRRTPGT AVPAHVRGEL NTAIIGLGDD GGYGGGWVPL
     VALKKVLRGI LKYLGVLWLF AQLPDLLREI LGSILKDNEG ALLNLDQEQP ALRFFVGGYV
     FAVSVHRVQL LLQVLSVRRF HHQAQQNGSS AAAQEELTQS EIGEICDYFS RRVASEPYDA
     SRVASFITLL TLPISVLREF LKLIAWKKGL SQSQQAGEIA PAQRPRIELC LENHSGTDLD
     NNCAAKSNIH YDRPHNTVDF ALTVVLDPVH IPHINAAGGA AWLPYCVSVR LRYTFGENPS
     VTFLGMEGSH GGRACWQRVD DWEKCKQRVS RTVEVNGSAA GDLTQGKLKL VADSVQRTLH
     LCLQGLREGG NNNNNTHQKE FTI