ID   MED14_ASHGO             Reviewed;         967 AA.
AC   Q75CZ2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=RGR1; Synonyms=MED14; OrderedLocusNames=ABR230C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 340.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016815; AAS51003.2; -; Genomic_DNA.
DR   RefSeq; NP_983179.2; NM_208532.2.
DR   AlphaFoldDB; Q75CZ2; -.
DR   SMR; Q75CZ2; -.
DR   STRING; 33169.AAS51003; -.
DR   PRIDE; Q75CZ2; -.
DR   EnsemblFungi; AAS51003; AAS51003; AGOS_ABR230C.
DR   GeneID; 4619289; -.
DR   KEGG; ago:AGOS_ABR230C; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_286680_0_0_1; -.
DR   InParanoid; Q75CZ2; -.
DR   OMA; MIDLLNW; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0001093; F:TFIIB-class transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 2.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..967
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304591"
SQ   SEQUENCE   967 AA;  110811 MW;  A000BCAAF0730E98 CRC64;
     MTIAVKESKI LYGNFDKAKS GIMKGSGGIV EQGPGLQQRI ITPAKEMPPE IPHVEFNQLP
     LSVLIRNLTV YAAKELSQYM KTNVRSTQDA STRKMEFLRL IIFLRNQFLR LYVLVKWCKT
     IRQNNFHTMI DLLNWFRGTN IIVNNCLLAL KDMSTSMAGA KLPNPDLITA LEVLMLGRPD
     LPTHGFLLTG DENGQQANKI PAKLILKRFR DLNTCLSVKI SLMDLPAEMH TYSIKDGRIT
     FTVFPEFEIS LSTIDRESPL FFVDVKFLFN ENKFPLNTTK IALLVNDILY KSPTPLLSLY
     QFLHRYVLTL QLYMIHVELQ DIETNGKYSG GNLAHHYDPK KNIISLRYWL QSKMNSKCKA
     LIGVEKTSQS IILQWHLPDT KEEGKTTKYN NLLGNIEAIL DEITFNHARI IMSELLDTGL
     FEGDDNKNDT SDTLFFHVPV ICVATAPVQL KINTISGIFY FKNPSALLLS YAKQLNQTSD
     LDDVVNILER LRMDKIVHIL RNMFEKTGWI CEDVVKLNKP ILYDKHKDKK RILTRDLFIR
     IKDWPANWFF ILNLVASGAT CIVEKLIGKV QSVKGTWEVK YLDQGNLQVS KLESITYQNV
     MHMQKTIIQK ILNHMIIDSL NELKISKVIC QGEASQKLPA YVQTNTAGAS NVSIIAIGLE
     SFLQGSKALS DTLESTIFLK MDYEQNEVKL FGKFKKDTQM IRYQCDDLLI NFIDKRGLAF
     HMTEDFTSLN HVVQYLTKFR QKLMQLVVLT DVTEKLHNNF RSEHFQIVKL RPNEISFRYL
     KNSKDDQDCT IQIVTNETKI EKLQVQLSPL NPQNIIQKYL ECDKYDPHFI FNYLHFTSKL
     FSAVKSIESI TNTNTMTITL HLHTLAAYQL SYHDRLTGGQ IALCIDLRNS PSRAGSMYHL
     YFAQEDYSAS KNPMYPAILQ VINNVFMLNN NTKRKVPSVI RLGTGVACPL GDIEPLLEEI
     HSILIRS