MED14_CAEEL
ID MED14_CAEEL Reviewed; 1516 AA.
AC Q03570; D2Y8V9; P34581; P39218;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 6.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE AltName: Full=Mediator complex subunit 14;
DE AltName: Full=Mediator complex subunit rgr-1;
GN Name=rgr-1; Synonyms=mdt-14; ORFNames=C38C10.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12089139; DOI=10.1074/jbc.c200305200;
RA Shim E.Y., Walker A.K., Blackwell T.K.;
RT "Broad requirement for the mediator subunit RGR-1 for transcription in the
RT Caenorhabditis elegans embryo.";
RL J. Biol. Chem. 277:30413-30416(2002).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC transcription in the embryo and for phosphorylation of the RNA
CC polymerase II C-terminal domain repeat. {ECO:0000250,
CC ECO:0000269|PubMed:12089139}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12089139}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=c;
CC IsoId=Q03570-3; Sequence=Displayed;
CC Name=a;
CC IsoId=Q03570-2; Sequence=VSP_038712, VSP_002442;
CC Name=b;
CC IsoId=Q03570-1; Sequence=VSP_038712;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC {ECO:0000305}.
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DR EMBL; Z19153; CAA79550.3; -; Genomic_DNA.
DR EMBL; Z29115; CAA79550.3; JOINED; Genomic_DNA.
DR EMBL; Z19153; CAA79551.3; -; Genomic_DNA.
DR EMBL; Z29115; CAA79551.3; JOINED; Genomic_DNA.
DR EMBL; Z19153; CBI83227.1; -; Genomic_DNA.
DR EMBL; Z29115; CBI83227.1; JOINED; Genomic_DNA.
DR PIR; A88554; A88554.
DR PIR; B88554; B88554.
DR PIR; S28289; S28289.
DR RefSeq; NP_499067.3; NM_066666.3. [Q03570-1]
DR RefSeq; NP_499068.3; NM_066667.4.
DR AlphaFoldDB; Q03570; -.
DR SMR; Q03570; -.
DR BioGRID; 41517; 2.
DR IntAct; Q03570; 1.
DR STRING; 6239.C38C10.5c; -.
DR EPD; Q03570; -.
DR PaxDb; Q03570; -.
DR PeptideAtlas; Q03570; -.
DR EnsemblMetazoa; C38C10.5a.1; C38C10.5a.1; WBGene00004343. [Q03570-2]
DR EnsemblMetazoa; C38C10.5b.1; C38C10.5b.1; WBGene00004343. [Q03570-1]
DR GeneID; 176320; -.
DR KEGG; cel:CELE_C38C10.5; -.
DR UCSC; C38C10.5b; c. elegans. [Q03570-2]
DR CTD; 176320; -.
DR WormBase; C38C10.5a; CE44376; WBGene00004343; rgr-1. [Q03570-2]
DR WormBase; C38C10.5b; CE44435; WBGene00004343; rgr-1. [Q03570-1]
DR eggNOG; KOG1875; Eukaryota.
DR GeneTree; ENSGT00390000001021; -.
DR InParanoid; Q03570; -.
DR OMA; KITPREQ; -.
DR OrthoDB; 1215335at2759; -.
DR PhylomeDB; Q03570; -.
DR PRO; PR:Q03570; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00004343; Expressed in embryo and 4 other tissues.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR013947; Mediator_Med14.
DR PANTHER; PTHR12809; PTHR12809; 1.
DR Pfam; PF08638; Med14; 1.
PE 3: Inferred from homology;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1516
FT /note="Mediator of RNA polymerase II transcription subunit
FT 14"
FT /id="PRO_0000096360"
FT REGION 22..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1434..1516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1441..1472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..128
FT /note="Missing (in isoform a and isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_038712"
FT VAR_SEQ 796..801
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_002442"
SQ SEQUENCE 1516 AA; 170401 MW; 37AD32A27CF447CF CRC64;
MSSNVLPMYA STPSRAFAHS KLSSQLRAAL ADNSPAAPIS PAPSGSALSL DESSGISDRD
VHSSQEQTAH LSEVDVKSIH SSDSEDEPAQ NPLPEVPANC GPPTIPLNVL LDFAIQHVYH
EITVLAELMQ RKTNDQGEQE RKMSLVHFAH ATRSQFLKLV ALVKWIRISK RMDVCYSIDY
LLDLQSQYFI DTADRLVAMT RGDLELARLP EYHIAPAIDV LVLGTYNRMP SKIKEAFIPP
AKITPREQKL VTSRLNQLIE SRLSRLSSGI PPNIKEIHIN NGLATLLVPG EFEIKITLLG
ETEMTKWTLL NIKILVEDYE LGMGLPLVHP LQLNQLHGVL QSRMNVSLNP IKEAFSFLHS
FCVSLQLDVL FCQTSRLAAG RLRDNITIEK YDPKERVLVV GYWVKRSKSR RLTVGQVKCD
AQYRVQIYED PNDKLGGLKV RHFPHAPQLG RLDSGAGMLS IDRLLSETYV VRCKERLMRL
RRILEAAEPR LEVKMTGISA PSLSLALLPD TSSKDEMMTV SVNSFCGKVL CNVHILSAEH
EDVLAFGKAL YSSQCSAHTI QMYLRKLRVA LVIERYRRSV KALPVREVQE AELLPFAKEC
LGDAPAQRII LQFLRSEDYY LLVTFSPDEK AVVKTHIQLL EVVGDRAQFI QLEDDEMNGM
HVKEAINQGT MRFSPSHKTS LQEECSREQR LAFAVATVED RITYMYLAAE LMKKGIGVDV
RKDSAHVPGG LALHITDVKN VVPFEASEFF ECCIRCCLRL DNRNRYTFQF EMCFENIPLV
RDVPHGLPHR RDGEPKDRTS KDATWLQELN HINQSSPEKL VELIIHRLMR YLYMYKVVHQ
FSLAYEKHFK NYCNIEAYTF HKLVVSYGDN RDMLMILAFN VKSQAPGSSE DFFFLNFGQS
MPHRQFNSTE IDWHQKPRWN PHSMMSQLMR DDLKETNDLV FTMHFLCETI RPLVAIGNFS
RIRFQSQKSL SQLIGPDVHF PFRLKYHLYA LDQTTLRLMQ GNVILEIKLL EGCKIAVRDV
SRYRPRCAGL FQLFSNIDSE TTAIMNDEIA IPQSDNPQTA GPTMWTPEQF MDSLDERPEE
IDPRMAITSQ PILMSHDTII KACDFKDTEG RITCPLDEYL CSISYLQRAL LTLERMSPRA
TLNKNSSSNL SCGFVTIIDA KPDFIRFRAS QMNGDGVNAT SMVHYKIYLC PVAMTLKIRI
EFEEGTNSAA TADNLKTLTT YFEKVVFPCG DEYALQSYIC LTRLTSFEAT QSIANLMNVQ
MEHPPTSKCC VQLSLTYNNT STKKLAPATK VDQPLQNIIF NVIVSQSRTS ESFSVLRFIY
RIKENFVVVP SANEKNKQMA DEVNAETKTS GGNPIWNLVR LVMDRFNSGD WNPGNRDEPI
ISSVAPPTYV NPGSVAGPSS VAAPGSVSIQ QPGSVLQPGS MMGPQSVNAL QYGMHRQPMG
GPQSMQMNPS SVGQPGSVGG PGSHQQHMMN PGSVGPGSVG GPGSVNPGSV GYPQWNPPSV
GQSYHHPLHH QQYPPQ
