ID   MED14_DICDI             Reviewed;        1420 AA.
AC   P0CB66; C7G072;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Putative mediator complex subunit 14;
GN   Name=med14; ORFNames=DDB_G0291297;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000177; EEU04048.1; -; Genomic_DNA.
DR   RefSeq; XP_002649100.1; XM_002649054.1.
DR   AlphaFoldDB; P0CB66; -.
DR   STRING; 44689.DDB0266830; -.
DR   PaxDb; P0CB66; -.
DR   PRIDE; P0CB66; -.
DR   EnsemblProtists; EEU04048; EEU04048; DDB_G0291297.
DR   GeneID; 8628070; -.
DR   KEGG; ddi:DDB_G0291297; -.
DR   dictyBase; DDB_G0291297; med14.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_253145_0_0_1; -.
DR   InParanoid; P0CB66; -.
DR   OMA; LISHEYC; -.
DR   PRO; PR:P0CB66; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1420
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 14"
FT                   /id="PRO_0000388653"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          449..490
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          750..799
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1389..1420
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1..43
FT                   /evidence="ECO:0000255"
FT   COILED          1382..1412
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1420 AA;  162896 MW;  04AE1AE4E0A58064 CRC64;
     MDQNQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQPPNGMMP MNGEQTTPII
     PQNRNISLSL VIHRLVEQSY NSLLGLTEGL PKANDLERKK AIVDYLDGTR EKFLRLMVLI
     KWSEHVPTLT KANNIIDILN LEDSYLREAA DLLINTQFSL VNARAPIYDV PTAIDVLTTG
     TYQRMPTNIK RVIPPPPLKP TQIESALERL NDIIKYKLFI SDVPKEFQPI TVSDGKAHIF
     VDDEYEAYLT IDGGSEKSNW VILSLNLFVY SKRNLNGEGP IKVAYDNKMK YVLDRVQNRI
     ISSAQPLFEL HNIVHYLCIS SQMDILASQV ENLKKTILKN NIRCVFGKDQ SITVFYWLPE
     DFNLVGVTQH TLGNLMPNKH TNFKIYIDEH QKIKISHYPP ITHPKNENYF KIASLNLETI
     LLQAIELNAY DKVYLLNSLL LDNRITANTT SSSSSSSSNN NNTASPIINR NNNNGKPNLL
     STKQSNNPLS RSFHLNDIKL IMSSRFSDEN QNDSNGNNDH LPTVLRVMLY GSKFLDITVN
     FQNGKFSLIK SSNYIEFTNH LEQRLNKDPN EIESIVNVFK LKSLLTCFEE ASLFLGLECF
     NKIPLQMNSS NNSESNQLAN ELFSDSNFIC VSISLAKENN PYYLVISIKA TCFTPSFHLL
     FCKMLPKSTI MTLDSIIKLE SDQLNKLLKE CPIGSISSSN NTNSNGNGPF QSYISTLLEK
     IVEASNQKIN LLSIQSFLKK ENINYYQPSQ QDIENNNNNN NNNNNNNNNN NNNNNNNNNN
     NNNNNNNNNN NNGNNGYKNN GITTTTSIVF LFNEQQIQKI SPFLSNHISH QTPITISFKN
     GFYTLSFTQQ RPFEYKKYGS GSNININSFG ENNSNENYIY RKGNWIFKYQ QTSDWFNSFC
     NDLMTISKIT NISSQLLKQM ETLEIYKQLI THLTIKPMSI EFVCFVGSNQ TRTNVIMFVE
     KKTAEIKLSF QPYSNPLLVY LEKDINQSPT NDITNSLRAI INSNDISVYI NSLISPLELS
     FYLPLEILVI PRSICQIRLL YKNLYGIDIK LISHEYCAIS DSFYSLNSSK QVRLTSINQL
     HSFMEQRVSL QALDNPTGHR TSWLLPIKQF QKTISRIFIY LNSLNTLKFA QNLMKPNFQP
     LVPSNPSSQK FSNDYFIVSF SIRDYTSFDI DVTNKNLENS VPSNEELALF CQYFKKKVQQ
     LNYRSQTIGS CIQMLTLPPK ILWEFIRVLL EITGPKFDGY TIEISLNTSS IHSKNKESFF
     HIPDENQVYF ILRYLNSSRT DIIPPDQFTD IPIIYNYNEK SIRYWNKLDS NSTSSSSSST
     LPKKSLSIVE EVKQKFVEEV AKLIPDALNA SSGKSSPISI FFKSFLPKIK PIFSNPTAST
     LLLIQQQQQQ QQQQQQQQQQ QQQQQQIENN NFASASSSIR