MED14_DROME
ID MED14_DROME Reviewed; 1553 AA.
AC Q9W0P8; Q5U1E1; Q8IRI9; Q961S0; Q9GYW6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 4.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE AltName: Full=Mediator complex subunit 14;
DE AltName: Full=dRGR1;
GN Name=MED14; Synonyms=Trap170; ORFNames=CG12031;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 214-1553 (ISOFORM 1).
RA Southworth J.W., Kennison J.A.;
RT "Transcriptional coactivators in Drosophila.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 214-1553 (ISOFORM 1).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP FUNCTION OF THE MEDIATOR COMPLEX, AND IDENTIFICATION IN A COMPLEX WITH
RP CDK8; MED6; MED14; MED17; MED18; MED20; MED21 AND MED31.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [7]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-615 AND SER-1015, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional pre-initiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA, MtnB and MtnD genes.
CC {ECO:0000269|PubMed:11259581, ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex, which may include CDK8,
CC MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22, MED27,
CC MED28, MED30 and MED31. {ECO:0000269|PubMed:11259581}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=A {ECO:0000312|FlyBase:FBgn0035145};
CC IsoId=Q9W0P8-1; Sequence=Displayed;
CC Name=2 {ECO:0000305}; Synonyms=C {ECO:0000312|FlyBase:FBgn0035145};
CC IsoId=Q9W0P8-3; Sequence=VSP_058119;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG02462.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAK84939.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF47395.4; -; Genomic_DNA.
DR EMBL; AE014296; AAN11445.2; -; Genomic_DNA.
DR EMBL; BT015951; AAV36836.1; -; mRNA.
DR EMBL; AF289997; AAG02462.1; ALT_INIT; mRNA.
DR EMBL; AY050240; AAK84939.1; ALT_INIT; mRNA.
DR RefSeq; NP_612041.2; NM_138197.3. [Q9W0P8-1]
DR RefSeq; NP_728534.2; NM_167835.2. [Q9W0P8-3]
DR AlphaFoldDB; Q9W0P8; -.
DR SMR; Q9W0P8; -.
DR BioGRID; 63628; 49.
DR DIP; DIP-60711N; -.
DR IntAct; Q9W0P8; 2.
DR STRING; 7227.FBpp0072453; -.
DR iPTMnet; Q9W0P8; -.
DR PaxDb; Q9W0P8; -.
DR EnsemblMetazoa; FBtr0072554; FBpp0072453; FBgn0035145. [Q9W0P8-1]
DR EnsemblMetazoa; FBtr0344912; FBpp0311212; FBgn0035145. [Q9W0P8-3]
DR GeneID; 38073; -.
DR KEGG; dme:Dmel_CG12031; -.
DR CTD; 9282; -.
DR FlyBase; FBgn0035145; MED14.
DR VEuPathDB; VectorBase:FBgn0035145; -.
DR eggNOG; KOG1875; Eukaryota.
DR GeneTree; ENSGT00390000001021; -.
DR HOGENOM; CLU_001928_0_0_1; -.
DR InParanoid; Q9W0P8; -.
DR OMA; SPAGSFM; -.
DR PhylomeDB; Q9W0P8; -.
DR SignaLink; Q9W0P8; -.
DR BioGRID-ORCS; 38073; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38073; -.
DR PRO; PR:Q9W0P8; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035145; Expressed in egg chamber and 15 other tissues.
DR ExpressionAtlas; Q9W0P8; baseline and differential.
DR Genevisible; Q9W0P8; DM.
DR GO; GO:0070847; C:core mediator complex; IPI:FlyBase.
DR GO; GO:0016592; C:mediator complex; IMP:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0060260; P:regulation of transcription initiation from RNA polymerase II promoter; ISS:FlyBase.
DR InterPro; IPR013947; Mediator_Med14.
DR PANTHER; PTHR12809; PTHR12809; 1.
DR Pfam; PF08638; Med14; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..1553
FT /note="Mediator of RNA polymerase II transcription subunit
FT 14"
FT /id="PRO_0000304588"
FT REGION 699..723
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1006..1199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1513..1553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 55..59
FT /note="LXXLL motif 1"
FT MOTIF 472..476
FT /note="LXXLL motif 2"
FT COMPBIAS 1100..1127
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1132..1156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 615
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1015
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 410
FT /note="D -> DCE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_058119"
FT CONFLICT 698
FT /note="T -> N (in Ref. 5; AAK84939 and 3; AAV36836)"
FT /evidence="ECO:0000305"
FT CONFLICT 822
FT /note="L -> Q (in Ref. 5; AAK84939 and 3; AAV36836)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1553 AA; 172341 MW; 71CF4DA8A23A5023 CRC64;
MAPTPLPLEQ MPGVGGGGGG YLPAAQEGGP RINTMSMSVL IDFIIQRTYH ELTVLAELLP
RKTDMERKVE IYNYAARTRH LFTRLNALVK WGNSVSKVDK SSQIMSFLDK QNMLFVETAD
MLARMSRETL VRARLPNFHI PAAVEVLTTG TYNRLPTCIR ERIVPADAIT PAEKRQTLLR
LNQVIQHRLV TGKLLPQMRE FRIRNGRVTF EVKHEFSVSL TVMGDNPTVP WRLLDIDVLV
EDKETGDGKS LVHPLQVNYI HQLIQARLVE NPNALSEVYN CLHYFCQSLQ LEVLYTQTLR
LNYERLDDNN ITVEEYVPGV KLTVSYWRDL KSELGYRLTV QSDPSEIGRP LAVVHVPSLG
AKESAEVADR AVRSEHLSME RLIVHTVYIR SVSRLSDLKL EFQAFLKDVD FNLQGTPAIL
TVPVLSPCLR AEQIHITIDT HTGMFRCHVP KHLDCPIMEE MQDCLNGDRS KLPALLSELR
FWITHRRCDK TLQHLPATAT ETLPFLVQPD QEILQPGRHK IYVKLHRHPN IVLVVQLKEK
TTMPNEMEYT FHLGFVAYQK DELDVIDDSA MQLVSIVAQP HSDIPKCYTK LMRLIEFDTF
VATHGPGTEV DAEVSPHKRK SNGDLLAPPA KQQKTIFPAY FIPELAHVVA MCDEKIPFMN
LAQTLSKHNI PHSGLQVEAN ATSLVLKILA LPQPGKTTFA TQQQQQQGAP AVAGENKPSG
TSGLPKIDSH VWDDLMRRVL SISIRSQTNK NSQVRIWVVE FVFYSTPLQS SHPKEQGSRR
TVYLTYEQAN YDFSKTVEDL LNDWSKIVYL YTLVYDFAEQ LLNKRLSLCD MLVVKSYSYM
NLLLGYGPKK EVSCNIYWSV QSHGFRLTFV GGMSAVNGHS MMRDQLAQHL NQQHSITQIA
QILHETYNPL SSIAKLPVLP FLGIPRPQVP VLSFCVLAQS PCLIRLTYQA VYCLELRFRA
NRLVSIRDGA SSRFERNVVE EFTPIQGLKA FLSKYVDESA VYRGRASHED DNPLSPMGME
DNFGGPSSVA GVSAGGSSPF LGTGMRGPQS PRDSGLRFPA PHTPPSSSNP HTPASPHPSA
GAGGGSGPQG HGNFNLTSPP APHMPHPSPS GLMPSSPLNP QPSPHMVHSP GPNTLYMQSH
QDSPFTAMSP ANNNWPGSPS MPRPSPRPGQ SPDHKSTGGG AGVAGGTDRG GSRGTLNRPW
AGAVPTLLTH EALETLCRPS PYPNKDINVP DMSPLERFLG CVYMRRQLHR NIQSEETLTA
LNSTEPGVVL FKVDGLQCQV VLNQMHMQTL HLKVSQLPPM PDKHPPPFQL SQDDLLVIEQ
YFDTRVAAPP YRPNSLHSIC RLLNLPAQVL KDFVQIMRLD LKPELGGDQL KWTVQICLRM
PPSAVPIVPS GNACVVMGRM KILFFLQITR IPYGAVIGVG KDWKDSPSLV LPIVYDIQTN
VTQLAERTGQ VISPTMTAAS TLLRRFAEFN AQQNQCTLFP AITDLLTNLQ LAAEMPQPPP
NQSIGPPVGV GVGVGSSPNP MMPMQQLPQQ VGPQGPVGPG GYPQMGPNPG GPQ
