MED14_DROPS
ID MED14_DROPS Reviewed; 1546 AA.
AC Q29FF7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE AltName: Full=Mediator complex subunit 14;
GN Name=MED14; ORFNames=GA11352;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC {ECO:0000305}.
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DR EMBL; CH379067; EAL31298.2; -; Genomic_DNA.
DR AlphaFoldDB; Q29FF7; -.
DR SMR; Q29FF7; -.
DR STRING; 7237.FBpp0276908; -.
DR PRIDE; Q29FF7; -.
DR eggNOG; KOG1875; Eukaryota.
DR HOGENOM; CLU_001928_0_0_1; -.
DR InParanoid; Q29FF7; -.
DR OMA; SPAGSFM; -.
DR Proteomes; UP000001819; Genome assembly.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR InterPro; IPR013947; Mediator_Med14.
DR PANTHER; PTHR12809; PTHR12809; 1.
DR Pfam; PF08638; Med14; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..1546
FT /note="Mediator of RNA polymerase II transcription subunit
FT 14"
FT /id="PRO_0000304589"
FT REGION 692..717
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1000..1193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1512..1546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 51..55
FT /note="LXXLL motif 1"
FT MOTIF 468..472
FT /note="LXXLL motif 2"
FT COMPBIAS 693..714
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1094..1121
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1126..1150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1512..1526
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1546 AA; 171861 MW; 33319D673F4ABED3 CRC64;
MAPTPLPLEQ MSGVGGYLPA GQEGGPRINT MSMSMLIDFI IQRTYHELTV LAELLPRKTD
MERKIEIYNY AARTRHLFTR LNALVKWGNS VSKVDKSSQI MSFLDKQNML FVETADMLAR
MSRETLVRAR LPNFHIPAAV EVLTTGTYNR LPTCIRERIV PADAITPAEK KQTLLRLNQV
IQHRLVTGKL LPQMREFRIK NGRVTFEVKH EFSVALTVMG DSQNVPWRLL DIDVLVEDKE
TGDGKSLVHP LQVNYIHQLI QARLVENPNA LSEVYNCLHY FCQSLQLEVL YTQTLRLNYE
RLDDNNITVE EYVPGVKLTV SYWRDLKSEL GYRLTVQSDP SEIGRPLAVV HVPSLGAKES
AEVADRAVRS EHLSMERLIV HTVYIRSVSR LSDLKLEFQA FLKDVDFNLQ GTPAILTVPV
LSPCLRAEQI HITIDTHTGM FRCHVPKHLD CPITDEMQEA LNGDRSKLPS LLSELRYWIT
HRRCEKTLQH LPATATDTLT FLTQPEQELL QQGRHKIYVK LHRHPNIILV VQLKEKSTMA
NEMEYTFHLG FVAFQKDEAD VIDDSAKQLV SVVAQPPSDI PKFFTKLMRL IEFDTFVATH
GPGTEVDAEV SPHKRKSTGD ILAPPAKQQK TIFPAYFIPE LAHVVAMCDE KIPFMNLAQT
LSKHNIPHSG LQVEANATSL VLKILALPQP GKSATAAGQQ PQQGAASAAG TAPPSGSSAF
PRIEPHVWDD LMRRVLSISV RSQTNKNSQV RIWVVEFVFY STPLQSCHPK EQGSRRTVYL
TYEQANHDFS KTVEELLNDW SKIVYLYTLV YDFAEQLRNK RLALCDMLVV KSYSYMNLLL
GYGPKKEVSC NIYWSVQSHG FRLTFVGGMS AVNAHSMMRD QLAQHLNQQH SLTQIAQILH
ETYNPMSSIA KLPVLPFLGI PRPQVPVLSF CMLAQSPCLM RLTYQAVYCL ELRFRANRLV
SIRDGASSRF ERNVIEEFTP IQGLKAFLSK YVDESAAYRG RAPHEDDNPL SPIGMEDNYG
GPSSVTGVSA GGSSPFLGAG MRGPQSPRDS GLRFPAPHTP PSSSNPHTPA SPHPSAGGGG
AAQSHGNFNL TSPPAPHMPH PSPGGLMPSS PLNPQPSPHM VHSPGPNTLY MQSHQDSPFT
AMSPANNQWP GSPSMPRPSP RPGQSPEHKS TGGSVVTGGP DRGGSRGTLN RPWAGAVPTL
LTHEALETLC RPSPHPNKDI NVTDMSPLER FLGCVYMRRQ LHRNIQNEES LTALNSTEPG
VVLFKVDGLQ CQVMLNQMHM QTLHLKITQL PPPPDKPTFQ LSPDDLLVIE QYFDTRVAAP
PYRPNSLHSI CRLLNLPAQV LKDFVQIMRL ELKPEFGGDQ LKWTVQMCMR MPPSAVPIVP
SGNACVVLGR MKILFFLQIT KIPFNGKDWK DSPSLVLPIV YDITMNLTQM AERREQVPSP
MMTAASTLLR RFSEFNSQQN QCSLFPAITD LLTNLQLATD MPQPPPNQAI GPPVGVGVGV
GVGIGVVGSS PNPMMPMQQL QPQVGPQGQV GPGGYPQLGP NPGGPQ
