ID   MED14_KLULA             Reviewed;         998 AA.
AC   Q6CRY2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=RGR1; Synonyms=MED14; OrderedLocusNames=KLLA0D05533g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382124; CAH00403.1; -; Genomic_DNA.
DR   RefSeq; XP_453307.1; XM_453307.1.
DR   AlphaFoldDB; Q6CRY2; -.
DR   SMR; Q6CRY2; -.
DR   STRING; 28985.XP_453307.1; -.
DR   PRIDE; Q6CRY2; -.
DR   EnsemblFungi; CAH00403; CAH00403; KLLA0_D05533g.
DR   GeneID; 2893173; -.
DR   KEGG; kla:KLLA0_D05533g; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_286680_0_0_1; -.
DR   InParanoid; Q6CRY2; -.
DR   OMA; MIDLLNW; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0001093; F:TFIIB-class transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..998
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304603"
SQ   SEQUENCE   998 AA;  114179 MW;  FEABD62837953746 CRC64;
     MTTDVAPGTM ENETSKVMSL KHKAAYVPPP IPHVEMNQLP LSLLLRNLTV FTAKEISQFF
     KLNVHTGGKT PFEKKLELLN MILFLRNQFL KLYVLVKWAK TLKQNNFHSL IDLLNWFRNA
     NMQVNNCTMA LKQILGTMAG AKLPNPDLAT SLEVLMLGRP NLPNHGFNLN GSQSDNLTIP
     NKLILKRLRD LNTCLSIKIS LMTIPSQLAK YEIKDGRIVF TVKDEFELQL STIDQNSPLF
     FVNINLLFNE RLPLNLARLE KHINEILFKS TNPLCLVYQF LHKYILTLQM YMIHVELNAL
     GLNGKYSGGH LVHNYDSKKQ IITIKYWLQS KIANTCKCVV GMDKETESIV LEWQNPDVNK
     ETVTTRYHGL LNNIESIIDE ITFNHAQMIR ADLLRTDTFQ EDNEDTTSTS LLFQIPTTCA
     TVSQIQLKID QVSGIFYFHN PSNLLLSYAR QINQSSNTQD LITVLGRLKL DKVDSILRHM
     LDKTGWICSD VVRLKSSIVP STDSTFSKDI FVKLKDWPSN WFLVLTIISS TNTCIVEKRI
     GKILSVKGVW KLKYMDKKNV ITSKLDTMTY PKMLTLQISI LNKIVNHMII DSLNELKIKN
     KICTFTTEDS NILPPYIIRK DGKENTDNIT VIALGLESFL EGSKALNTIL ESSMLLKIDY
     QKMDIELYGK FKTDNEMIRC QCDELSIKFL EEDSLSFFMS ENFGNLNDIM LYLSKFRKKL
     TQLIALTDVM DTLHSNFQSM DFRVVELRPN EIRFKYLPMK DSPDNEDCVI RIVTNQEKVE
     KLDIKLSEHN PQAMIQRFLD EKEGFAHNFI FHYLQFTLPL FRASKEADYT SSSPSTVKMH
     LFMHSLQEFH FLYRNHKVGG ELSFIIQLRS VVRTRHQENT QYFVRFAQDY SQPQLQQQHP
     LSKAIAEIQK NAFSLALLKE QNSNTTSSTT TAGTATAGAT MISTASSNKL LDLQDSSPTN
     AAVDWSSIKC VRLGSALACS HDQILPLLLQ FHKSISDC