ID   MED14_NEUCR             Reviewed;        1274 AA.
AC   Q7S154;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=rgr1; Synonyms=med14; ORFNames=NCU09112;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; CM002236; EAA29085.3; -; Genomic_DNA.
DR   RefSeq; XP_958321.3; XM_953228.3.
DR   AlphaFoldDB; Q7S154; -.
DR   SMR; Q7S154; -.
DR   STRING; 5141.EFNCRP00000006455; -.
DR   EnsemblFungi; EAA29085; EAA29085; NCU09112.
DR   GeneID; 3874469; -.
DR   KEGG; ncr:NCU09112; -.
DR   VEuPathDB; FungiDB:NCU09112; -.
DR   HOGENOM; CLU_003573_1_0_1; -.
DR   InParanoid; Q7S154; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1274
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304606"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1056..1142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1155..1274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1071..1091
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1119..1142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1155..1245
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1274 AA;  142414 MW;  0FD1636C7A96633D CRC64;
     MENGHMNGVR THHDRNSWTN GVNGGVAKRE GSPDKGKAHA NMSGSSVMMN GGGASFMDVD
     SQPPGGTKFN DLPDEIQHIT ADIMPLSLLL TRLSQWTHTK LQDEIAYLAS KPLPQGVLNG
     TTNHQSADNH DTSQESLEKK VHLLNFLQDM HSKWVKTLVI TEWSRKAAQV GALIDIRVHL
     LTKQAHYQEL FWELIRMKQD LHWAKVPGPD LKTALEVLTT GQASWMPELG YIEPPPITWE
     EKEAFIDNIN TMLSVRLTFD EHEKLPAAFR DYNIANGRVT FKVKGEFEVD LTIGDEDFEQ
     QFWFIDFRFL FTPAPAELSH GVRTFLENKV NTILGDQGLA GCYNYLHEFV LTQKIIEFRR
     QATELAMGRW VDTLKVEKLN RAMSIQYWLK PPHGLEGPSS QTAKSWILLG VWSGKGVEGD
     LDSKPSSYIS LRWFRDNKEV KDFDIPLNVE TISAEKLLTA VIASHVEYIL RSISSKLLSK
     PRFAQKHARL DLEISKEEPQ NSSLAVQLFD NDKAVIKIEP LTGFFTMFPR SPVFLKGQMK
     LNTSSNVAEE GANLMEQLRF DHAVKDLNSR VRSIGWFVCR PPITQEETKT IVYSDASGSR
     EAFKAVWLRK ANWMTRQWFV MISMSLGGDQ WWLVDLSPPK PNFPAGRLRL FTKMPMTSNQ
     LTLSDTFFRN LSVYAAGMIS HITDLRKLHT LKKSHTALEL PNHSLPPQVR LPTIYVRLSE
     MLQQRPGSSS RTLTWAKDFV PIIFKGVRSH TGDQEGPADA TAVRRDTPAS IRAEARLTVI
     DRNKFKSLRG NVDHDVVYNH RTGQFSLKLR ADMGTPVVDL LADRVQALER LMEFVEAIRL
     AGSNAIPQSV ALREVVFTYS NDPAEPVVGP PQGPRAWNVH LDLPKDAKVV LTLEKGNPHI
     RALDMLQDMA QSTKTELLPS YLPFSLPLYR VLDRIEDEWE SIAAKNAGNV AIVVKAAEWV
     TVRFTLPAPT TRRLLCLDIK TMGRKGRLLW HVKRTDEKGH ERPLNAPQDE FDTLLQRSVW
     HAHGDGRKTF GTSAAADPKV GIETLLWSIS ESVKSLVGTL PPPPGTSPHP GGGTQDLQQG
     PQKTPQEMVA AQAAQAAQAA QAAQGMPPQR PKQQPPTPSQ PQQQHRNVNQ PQAQAQPQLH
     TQVQLQQAQV AQLQAQRQAQ ARANNSSNNN NTFVNRAHPG QPPQQRQSGP QTQAQQQQQR
     YQIQQQAQAM QRMQQQQQQQ AAQQHAQQQG QGPQGQRSVH GQAGPQGVPQ GQPGHGGGAN
     GGMGGKNAPV VVID