ID   MED14_PICST             Reviewed;        1112 AA.
AC   A3LZ54;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 3.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=RGR1; Synonyms=MED14; ORFNames=PICST_63118;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABN68280.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000501; ABN68280.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001386309.2; XM_001386272.1.
DR   AlphaFoldDB; A3LZ54; -.
DR   STRING; 4924.XP_001386309.2; -.
DR   PRIDE; A3LZ54; -.
DR   EnsemblFungi; ABN68280; ABN68280; PICST_63118.
DR   GeneID; 4840708; -.
DR   KEGG; pic:PICST_63118; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_283151_0_0_1; -.
DR   InParanoid; A3LZ54; -.
DR   OrthoDB; 1215335at2759; -.
DR   Proteomes; UP000002258; Chromosome 7.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1112
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304607"
SQ   SEQUENCE   1112 AA;  128367 MW;  99E06EE6EE332A29 CRC64;
     MDEIHGEVSP PAINRSNGAL VSDNISQVVE GVDNVERPAK VAKGYSAANR PPPELPHITN
     NVIPLSNVLK FYTQEAFKQI TTLVENLAST KDSEDDSVRK KKFLELIIAL RQDFIKIYTL
     VKWAANSKDV SRFIDLLNWL RMQEFYFDQL TFQLNALNGY SGAKLPNSDL ITSLEVLFKG
     RPQLPSYNYI KIPKISPEKT LEVMQDLNLI LMTRIALIDI PKRFINNYVI KDGRIYFSVP
     NEFQVSITVA NDMIIESHDE YTKSPFYFID FKFLFGINPD TSLITHRDNK IVTKLPRSSH
     ENLEKIVNAT LLNQGLHGLY DLLHKFSISF KLYLIAKQLK ELQINTRWRN NLQVNYQNGK
     SLIILNYWSG QYLSKGWKSF IELGIDRNYN LNFRWFKNGK YNVNTELSAL FNKHLKNTNE
     GEEEKDDSTD DIEEPEDLNV DLILNIVVNK HSELLMSKVF NSLDAKLNTT DNPDQISYIT
     PHQLLIKSSP TKSTIFCINP LTGLFYFIDP CPVQNLIAKK INSPPTVVKN KNFVAESDMV
     NTIVDGLTQL RLEIFNKEIH NRLLTTEWIN NDIIGLNDYE ISRLSNFFAN SLDYNLNVNK
     IQFYRRKNWP SSWFLINLVS GVTSVTFWWV ARIKSVSGEW KIQWIQKLHL NEKTPSVEIT
     DDPTNLNFEF FNNLSTACSN MIIDHMLLEE LFKRNIKFLK VDNTALILQK FNIDKIPDDE
     DTQDTDDKNK PLIYESIIMI YNDNNLLPVY NSATSLFLKI KLINLNNLTQ MKLKLFGKLR
     NLSIRNSPEN FLQLNLKIDE IKNFFEIDDL INLSSRTNGS DSASSATTNH LLDKIFNNLN
     KLNKLIKILD QLNKNKIQII NNSVNDIIIK IDDNLDNLII KLPEKATDSI KLISEEEVKD
     GGNPEVKLIL DYLNKYLSNY YSDDFFANDE DKFYDGNKKT SIVGIIKYLK EINPILKSVQ
     TIKAKLRKPE SAFKLSNGLS KLNFDIKFTT LNLIQYVFHM NYQTPSSSKK IFKDKIVISL
     SFKNNKFDKV SKNLIKISLK DNLNSKNLKY KKLFELIFKS INDGSMDAHA HQLIKLNYDF
     LVNSSLVGEL MVRIANCFVL YLQAESNGRY NQ