ID   MED14_USTMA             Reviewed;        1493 AA.
AC   Q4P0P3; A0A0D1E253;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 2.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 14;
DE   AltName: Full=Mediator complex subunit 14;
GN   Name=RGR1; Synonyms=MED14; ORFNames=UMAG_10778;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 14 family.
CC       {ECO:0000305}.
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DR   EMBL; CM003143; KIS70234.1; -; Genomic_DNA.
DR   RefSeq; XP_011388380.1; XM_011390078.1.
DR   AlphaFoldDB; Q4P0P3; -.
DR   STRING; 5270.UM06320P0; -.
DR   PRIDE; Q4P0P3; -.
DR   EnsemblFungi; KIS70234; KIS70234; UMAG_10778.
DR   GeneID; 23566763; -.
DR   KEGG; uma:UMAG_10778; -.
DR   VEuPathDB; FungiDB:UMAG_10778; -.
DR   eggNOG; KOG1875; Eukaryota.
DR   HOGENOM; CLU_246884_0_0_1; -.
DR   InParanoid; Q4P0P3; -.
DR   OrthoDB; 1215335at2759; -.
DR   Proteomes; UP000000561; Chromosome 4.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR013947; Mediator_Med14.
DR   PANTHER; PTHR12809; PTHR12809; 1.
DR   Pfam; PF08638; Med14; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1493
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   14"
FT                   /id="PRO_0000304608"
FT   REGION          1..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          71..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          408..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          674..693
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          894..913
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          957..997
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..38
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        957..971
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1493 AA;  164482 MW;  E33A9D5C1E5DFF1D CRC64;
     MPSSPYGANA STSHQHVKQN AHPSAPLSRT SPSLPPKPTV AMNGNGHYHA APTSVALDKN
     KQTATSSHNL MIGVPPAATP NLSLAQSRPP DSKQSSDADG HPVFLSSAKG KQKQVDPIHI
     PLAQLEQELP LEEADLISLA ALVERLANFG YEALQNLAET LPSLPSSSKR AKIFNTALNV
     RKQFIKLLVL ARWSKDISDL QKSRNIIALL SEQQWQHEDV FAGLTDIRKI LPNARMRNAD
     LPTAIDVLHT GTYRRLPASI KQMAVAPKPF TDQQALLIVS RLEDALRTRM ACRELVPAPL
     SNYTIHDGKV HFHVPGLFQA QLTASGSAQH ELTPSSSDDR WWLLDLTFDV IASGSCAASC
     NRAFPRKPKR AYRERLRVWG DQQLSPVIQS DGVDESNARP LDIAADKTEQ GTDVQIAPEK
     PTDAPTVNEA NPAITATHHD DSLMPTAPVQ RDAPLSRLFA FLQERALHYQ MDILQHQAYE
     LCRLNWGSNL RIETAERPRN LTVHYWTQAQ GAAGATQRVE SAAAGGSVQI TIVDLPDTPG
     ATETLAALFD GDDDQSSTTH DTASLTTVKR RGLQVTWDAD RSILADAQSN NLAISPHSLD
     IEALLSSVIK QHTLALLRNL QRRILVSDHA LSRLLRPEDC TLCLEHIQRQ GDEFSTATET
     TTFNFLQIDL HASQRPRTAK SKPNPKPEQR SASCLAPLRL SVDPVTGRLL LDSEPAQSTT
     DLSSEQTSQQ AASFTASILT TRPNYARLAE ASDRINESID ALLDVLYRLD VFARVQEWER
     MASYLGLRSV RKLALRPQDL AKLGASTLAS AEAAPQLFLP LRQSFGGYFL ALQPSELAGV
     SIALVYVVQV MDPTGTPALS VQSIEWLDRA KIAAAASKVG SSGEDVGQEF VATEAGTKRK
     APPGVQGETN DVDANFGSSE LTIEELADVH SYCIALVSYF RVEQQLRMRG LPYLHVGSNT
     MRSSPPCKRQ RRQQTADARD DGLFGEDASG GTASSDDEAF AGPAGVAGVA ALVPSLCLRS
     IDVLGPSKSH LAKPNISLRV RHWDDDEKLC VEMRIKLRMK SRQFRTLHEV VALAASSDQQ
     STCALTWIDF DREASLLTLT TRDLDNCMSI FHTHWERVMR MVQLTREVLN ASRAWQQRAL
     RLRRSCKKPG ERVELCRFEL DSVVFSYGSI KVDGLEAKLL VRVRWQDAKL EMQAFSPIGV
     TQSGGYILEF GSMRSVQVEA LDQDAQLRED GVSRWFDETH CLANPHKVMA LELRRTVNVA
     ARSAAMHAML QPQAERIVWR GFLELLQHTL PLVREMAPLV DKCLTHAHVP EVEIKSATWF
     RFRFQDRYAI DVRLATRSRL VVSDASRALF ASEVRTSESE SESETTDAVP LDAVFGGSQL
     LNDLLDATTL ARKCSKPKTR IAAQLSSAQF GPIPNIEHVL RSVHAKLDAI TWIDARDHRS
     SAARLRVYEL GRALLISLPS ASHPNAQAAY HAIAHLVPLL IDRVEAQLAR STP