MED15_CAEEL
ID MED15_CAEEL Reviewed; 780 AA.
AC Q21955; Q8MPZ3;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Mediator complex subunit 15;
GN Name=mdt-15; ORFNames=R12B2.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, INTERACTION WITH NHR-49 AND NHR-64, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=16651656; DOI=10.1101/gad.1395406;
RA Taubert S., Van Gilst M.R., Hansen M., Yamamoto K.R.;
RT "A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism
RT by NHR-49-dependent and -independent pathways in C. elegans.";
RL Genes Dev. 20:1137-1149(2006).
RN [3]
RP FUNCTION, AND INTERACTION WITH SBP-1.
RX PubMed=16799563; DOI=10.1038/nature04942;
RA Yang F., Vought B.W., Satterlee J.S., Walker A.K., Jim Sun Z.-Y.,
RA Watts J.L., DeBeaumont R., Saito R.M., Hyberts S.G., Yang S., Macol C.,
RA Iyer L., Tjian R., van den Heuvel S., Hart A.C., Wagner G., Naeaer A.M.;
RT "An ARC/Mediator subunit required for SREBP control of cholesterol and
RT lipid homeostasis.";
RL Nature 442:700-704(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC regulated expression of genes controlling fatty acid desaturation by
CC factors including sbp-1 and nhr-49. {ECO:0000250,
CC ECO:0000269|PubMed:16651656, ECO:0000269|PubMed:16799563}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with nhr-49, nhr-64 and sbp-1. {ECO:0000250,
CC ECO:0000269|PubMed:16651656, ECO:0000269|PubMed:16799563}.
CC -!- INTERACTION:
CC Q21955; O45666: nhr-49; NbExp=4; IntAct=EBI-318240, EBI-318820;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q21955-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q21955-2; Sequence=VSP_020624;
CC -!- TISSUE SPECIFICITY: Expressed in the intestine and head neurons.
CC {ECO:0000269|PubMed:16651656}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:16651656}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; FO081686; CCD73316.1; -; Genomic_DNA.
DR EMBL; FO081686; CCD73317.1; -; Genomic_DNA.
DR PIR; T16726; T16726.
DR RefSeq; NP_741151.1; NM_171134.6. [Q21955-2]
DR RefSeq; NP_741152.1; NM_171867.4.
DR AlphaFoldDB; Q21955; -.
DR SMR; Q21955; -.
DR BioGRID; 41039; 23.
DR DIP; DIP-38923N; -.
DR IntAct; Q21955; 14.
DR STRING; 6239.R12B2.5a.1; -.
DR EPD; Q21955; -.
DR PaxDb; Q21955; -.
DR PeptideAtlas; Q21955; -.
DR EnsemblMetazoa; R12B2.5a.1; R12B2.5a.1; WBGene00007016. [Q21955-1]
DR EnsemblMetazoa; R12B2.5a.2; R12B2.5a.2; WBGene00007016. [Q21955-1]
DR EnsemblMetazoa; R12B2.5a.3; R12B2.5a.3; WBGene00007016. [Q21955-1]
DR EnsemblMetazoa; R12B2.5b.1; R12B2.5b.1; WBGene00007016. [Q21955-2]
DR EnsemblMetazoa; R12B2.5b.2; R12B2.5b.2; WBGene00007016. [Q21955-2]
DR GeneID; 175817; -.
DR KEGG; cel:CELE_R12B2.5; -.
DR UCSC; R12B2.5b.2; c. elegans. [Q21955-1]
DR CTD; 175817; -.
DR WormBase; R12B2.5a; CE30108; WBGene00007016; mdt-15. [Q21955-1]
DR WormBase; R12B2.5b; CE25078; WBGene00007016; mdt-15. [Q21955-2]
DR eggNOG; KOG4274; Eukaryota.
DR InParanoid; Q21955; -.
DR OMA; CVSKDEY; -.
DR OrthoDB; 727190at2759; -.
DR PRO; PR:Q21955; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00007016; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0016922; F:nuclear receptor binding; IPI:WormBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:WormBase.
DR GO; GO:0019217; P:regulation of fatty acid metabolic process; IMP:WormBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:WormBase.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0030730; P:sequestering of triglyceride; IMP:WormBase.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR019087; Med15.
DR Pfam; PF09606; Med15; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..780
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15"
FT /id="PRO_0000065434"
FT REGION 1..124
FT /note="Interaction with nhr-49"
FT REGION 2..96
FT /note="Interaction with sbp-1"
FT REGION 91..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..120
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..140
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 572..597
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 94..96
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_020624"
SQ SEQUENCE 780 AA; 84528 MW; 4259A33584603D0A CRC64;
MSEEDWPSPK FREHVIQRLE PELARNRQNA PNLPVPGDAR QVEEYVFAKC MSKDEYMRTI
AKVINAINCN SKSAAVPSVL QPSQFHSPPC TTAALLGNTP AGGTPGYRAP VPPDPQPTSA
QARNPPVTVA TTQASTTPSA PNPPGGLPAP SASATAAVAA AVASFPSPDT SIRPGGQITP
GSQAPGGGPT PAPNVPFPNG SSQMNGGPAM GQPPPQMGAP NMGGPPNGYG GYGMMNGPPG
SGAPMGGNPY NQQIKKDMDQ ARPWDPSAHM YQQQPQWGAM PPQQPHGYPG RPMNGQGATP
TGPSSVLESL INQPQQYPGH HNQMGPPGDR NVAAQRAAAQ QQQQQQQQQQ QQRPGMVPNQ
GMMSSEDQTV YSAKLRNMRG SCDSLRTRAR QCRHEGNHEA AHKLEVMLSV LEGKRVVSLE
YLNHLEMWIA RKQDFLNIAP MSQNQNHMGM NDPMMNGEHA MLGNGQVPNP YGGHPGYGHQ
QYMGPPPPHM QMHQPPMWHQ QQHQQQQRMM PQDHMMMQGG GGPVHGMYRG DMGHDPMTSP
VNNHRHAPYP NPAAMRNNMR MPNGPGPIGR DRNSMSGSSM SGPSSGAPSM NPMGTPNQKM
GTPGSMGGMS GLDDLNYDDF LPNPTPIDAL QPTLHVGQNS MNAGPPVQRS NLNETARKEL
QILDARFEID PNHQRHDANH IIVVCKIRNQ QFPPLRLVVP TTYPAGNVTV DRAVIDLDAY
LYDDLQNSVY ERLSRPGLSS ITDYLNAWEE QVNQYQNQTS GGLDVAFNVG NDFFYDNLNL
