MED15_CANGA
ID MED15_CANGA Reviewed; 1095 AA.
AC Q6FRS9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Mediator complex subunit 15;
DE AltName: Full=Transcription regulatory protein GAL11;
GN Name=GAL11; Synonyms=MED15; OrderedLocusNames=CAGL0H06215g;
OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS Y-65) (Yeast) (Torulopsis glabrata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC Nakaseomyces/Candida clade.
OX NCBI_TaxID=284593;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; CR380954; CAG59998.1; -; Genomic_DNA.
DR RefSeq; XP_447065.1; XM_447065.1.
DR PDB; 4D7X; NMR; -; A=1-86.
DR PDBsum; 4D7X; -.
DR AlphaFoldDB; Q6FRS9; -.
DR BMRB; Q6FRS9; -.
DR SMR; Q6FRS9; -.
DR DIP; DIP-59822N; -.
DR IntAct; Q6FRS9; 1.
DR STRING; 5478.XP_447065.1; -.
DR BindingDB; Q6FRS9; -.
DR ChEMBL; CHEMBL4295608; -.
DR PRIDE; Q6FRS9; -.
DR EnsemblFungi; CAG59998; CAG59998; CAGL0H06215g.
DR GeneID; 2888816; -.
DR KEGG; cgr:CAGL0H06215g; -.
DR CGD; CAL0131746; GAL11A.
DR VEuPathDB; FungiDB:CAGL0H06215g; -.
DR eggNOG; ENOG502QVXD; Eukaryota.
DR HOGENOM; CLU_009962_0_0_1; -.
DR InParanoid; Q6FRS9; -.
DR OMA; KYWESMK; -.
DR Proteomes; UP000002428; Chromosome H.
DR GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR GO; GO:0001095; F:TFIIE-class transcription factor complex binding; IEA:EnsemblFungi.
DR GO; GO:0001097; F:TFIIH-class transcription factor complex binding; IEA:EnsemblFungi.
DR GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR GO; GO:2000219; P:positive regulation of invasive growth in response to glucose limitation; IEA:EnsemblFungi.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0070202; P:regulation of establishment of protein localization to chromosome; IEA:EnsemblFungi.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR CDD; cd12191; gal11_coact; 1.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR033789; Gal11_coact.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR036546; MED15_KIX.
DR InterPro; IPR008626; Mediator_Med15_fun.
DR Pfam; PF18535; Gal11_ABD1; 1.
DR Pfam; PF16987; KIX_2; 1.
DR Pfam; PF05397; Med15_fungi; 1.
DR SUPFAM; SSF47040; SSF47040; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1095
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15"
FT /id="PRO_0000304671"
FT REGION 66..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..323
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 448..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 737..847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1050..1075
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..197
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 448..505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 748..847
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1050..1066
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 2..5
FT /evidence="ECO:0007829|PDB:4D7X"
FT HELIX 9..29
FT /evidence="ECO:0007829|PDB:4D7X"
FT HELIX 34..52
FT /evidence="ECO:0007829|PDB:4D7X"
FT HELIX 58..84
FT /evidence="ECO:0007829|PDB:4D7X"
SQ SEQUENCE 1095 AA; 121152 MW; 903F3DF1ADC85348 CRC64;
MSSKETIPMH QRSQNVAELL TVLMDINKIN GGDSTTAEKM KVHAKSFEAA LFEKSSSKEE
YQKTMKSKID AMRSTRDKRK RESVGSASMM ANLGQDGTNN NNNNNNNNNN NLNMAASFMG
GDMFGRNQSP AQNSNANTNL NTNVGPGVNG PNGNDGTANP QMFMNQQAQA RQQAAARQLK
NRQMGGSSAQ QQQLTQQQQQ LLNQMRVAPI PKELLQRIPN LPPGVTTWEQ VTALAQQNRL
SAQDMSIAKD IYKIHQQYLI KAKLQQQQQR QQQQRQQGNP DVNNNMAGSN NNNNNNLPMA
QQQMQQRQQQ QQQSQQQQNR NPNQRHNVLS QINQMFTADE QRALLQEAME ACKNFQKTHF
GGQMSDANKQ AFIKKFINSK ALKKLEAMRM AQGGNNNANL NKGQADMLQR QQANMQMNQQ
QQRAAQNQRR GPVMNDAVSQ GYNNQMNSAA DSTMNNSNQP MNIGNNGVNM IPNQSQQQQQ
TNRPKEQTPQ QPQQRIQSNR SVPMLNPTPE DVEVVRRISA EAAKTQLRLT DLTNSLTPQE
RDEIKKRLQK NQQLFAQVSS YAPQVYLFTK SESFLKEVLQ LRIFIKEILE KCSKGIYVVK
LDTVDKLVIK YQKYWESMKI QLLRRQQLLQ QQQQQQQQGM DPNRAQNSQQ QQQQNQANMQ
QARNRKPTKN QTTPAIAASV AMNMNDKGAS MSPALQKAGS AVPNFAQQMS PNMTPGTIPP
TNVLSPHSQS HIPMVSPTMA KAASAAALKN DTASSSRRGS TKPRGKSTAP VTGKKTSNAP
TPQVVPATVP STTNLSAAGT PNIRNKSATP LTAGLSPKST IRSNSNTALA SAKTPSPMTV
SIPQPGNSSV FKKEEEYLSK LQLRKEEIRF RQKQRLDILS SSPVDLFLTT VADCLGINDE
EIELINKIPE TTADNINNTG KKKLTKAAQK LRDKEILNVS IQVGEKDKLI MSSKAPDKVM
DYSISAMSLA AVFKNLSSTG SLNNIALSGS NATTSKDIGN IYSHTGGVKR KFDEVEISPN
SNGSPSASIM SESKKIKIDS PEDMFVTHSS EAAKGTNNSS LMDSGKEGSC KSMAGSATEV
NDTSIWDWNF WTSIE
