ID   MED15_DANRE             Reviewed;         809 AA.
AC   Q7ZVN7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE   AltName: Full=Mediator complex subunit 15;
GN   Name=med15; Synonyms=pcqap; ORFNames=zgc:55839;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=17208216; DOI=10.1016/j.ydbio.2006.11.034;
RA   Lin X., Rinaldo L., Fazly A.F., Xu X.;
RT   "Depletion of Med10 enhances Wnt and suppresses Nodal signaling during
RT   zebrafish embryogenesis.";
RL   Dev. Biol. 303:536-548(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. Required for cholesterol-
CC       dependent gene regulation (By similarity). Positively regulates the
CC       Nodal signaling pathway. {ECO:0000250, ECO:0000269|PubMed:17208216}.
CC   -!- SUBUNIT: Component of the Mediator complex. Interacts with srebf1 and
CC       srebf2. Interacts with smad2, smad3 and smad4 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC       {ECO:0000305}.
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DR   EMBL; BC045472; AAH45472.1; -; mRNA.
DR   RefSeq; NP_998238.2; NM_213073.2.
DR   AlphaFoldDB; Q7ZVN7; -.
DR   SMR; Q7ZVN7; -.
DR   STRING; 7955.ENSDARP00000003782; -.
DR   PaxDb; Q7ZVN7; -.
DR   GeneID; 406346; -.
DR   KEGG; dre:406346; -.
DR   CTD; 51586; -.
DR   ZFIN; ZDB-GENE-040426-2032; med15.
DR   eggNOG; KOG4274; Eukaryota.
DR   InParanoid; Q7ZVN7; -.
DR   OrthoDB; 1424577at2759; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q7ZVN7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   Gene3D; 1.10.246.20; -; 1.
DR   InterPro; IPR036529; KIX_dom_sf.
DR   InterPro; IPR019087; Med15.
DR   Pfam; PF09606; Med15; 2.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..809
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   15"
FT                   /id="PRO_0000304664"
FT   REGION          115..137
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          183..211
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          413..549
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        432..463
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        464..488
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        497..511
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        534..549
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   809 AA;  89863 MW;  F1195483D42E1B93 CRC64;
     MDVPGPDSDW RSPAFRQKVV AQIEEAMRKA GTGHTKSSTE MESHVFTKAK TREEYLSMVA
     RLIIHFRDIH KKAQGGPDPI NALQNLPGGV PGVIGPRPPG AQMGGMGQMS MGPHAMQGVA
     GGQQGAGAAG PMQQMIQQQQ QQQQQQQQQQ SIQFQTFQPQ QQQQATMQSQ QPSAMFQQIR
     LQQLQQHHQN QQMQHQNQQQ QQAQNQQQQN QLHQTRMQQQ LQLQQLQQQQ QQQLHQQQVH
     AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQSI QQMQQQQQLQ
     VQAQGQPQVQ GQGGAVQMPP HSQQQQVLVP QMVQGQHSQM SALSQQQQLK LQQAMQARMQ
     QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQHQQQQVQ QVQQASQLTA VPGQMMPRPG
     MQIPPRLPRA TPNSAIPQNP VAIGGQQMPQ AQQMMSSPSP VQVQTPQSMP PPPQPQPSPQ
     PPSSQPNSVS SGPTPSPGGF QPSPSPQPSQ SPASSRTPQS YPLQVPSPGP LNTPGNPSSV
     MSPAGASQSE DQLYMDKLRQ LSKYIEPLRR MINKIDKNED RKKDLSKMKS LLNILTDPNT
     RCPLKTLQKC EIALEKLKND MAVPTPPPPP VPCTKKQYLC QPILDAVLAN IRSPVFNHSL
     YRTFAPAMTA IHGPQITGPS IPSRKRKLED DERQAIPNIL QGEVARLNSK FLVNLDPSFC
     SNNGMVHLIC KLDDKNLPSV PPLQLSIPAD YPDQSPHWED DGQQYEANPF LRTVHKNMTS
     KLLQLPDKHS VTALLNTWAQ SVRQACLSA