MED15_DICDI
ID MED15_DICDI Reviewed; 851 AA.
AC Q54B46;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Putative mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Putative mediator complex subunit 15;
GN Name=med15; ORFNames=DDB_G0293914;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NOMENCLATURE.
RX PubMed=18515835; DOI=10.1093/nar/gkn349;
RA Bourbon H.-M.;
RT "Comparative genomics supports a deep evolutionary origin for the large,
RT four-module transcriptional mediator complex.";
RL Nucleic Acids Res. 36:3993-4008(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000224; EAL60456.1; -; Genomic_DNA.
DR RefSeq; XP_628870.1; XM_628868.1.
DR AlphaFoldDB; Q54B46; -.
DR STRING; 44689.DDB0266901; -.
DR PaxDb; Q54B46; -.
DR EnsemblProtists; EAL60456; EAL60456; DDB_G0293914.
DR GeneID; 8629486; -.
DR KEGG; ddi:DDB_G0293914; -.
DR dictyBase; DDB_G0293914; med15.
DR eggNOG; ENOG502RSSW; Eukaryota.
DR HOGENOM; CLU_335390_0_0_1; -.
DR InParanoid; Q54B46; -.
DR OMA; WFKNPAL; -.
DR PRO; PR:Q54B46; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032021; C:NELF complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:dictyBase.
DR InterPro; IPR044607; RKD-like.
DR PANTHER; PTHR46373; PTHR46373; 1.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Cytoplasm; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..851
FT /note="Putative mediator of RNA polymerase II transcription
FT subunit 15"
FT /id="PRO_0000388654"
FT REGION 79..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 231..307
FT /evidence="ECO:0000255"
FT COILED 790..827
FT /evidence="ECO:0000255"
FT COMPBIAS 102..212
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 851 AA; 98453 MW; 87CF8AAA341294F2 CRC64;
MTDWKTTITP EERNGNSIKI LDFLMQWYPN FNRAEALVRA NTLEDRIFNT PGIISKNDYI
ERIAKKIYAI GESLEAKKAQ AGMTNNPQTP NPMPTTPITP LIQQPITPVQ QQVPPQKQPQ
PVYNQQQNQQ QNQNQYTTTY PSPNTPQQSN TPPISNNNSN NNSNNNLNNN NNNNINNNNN
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT VYAQPLTPQQ QIQQQQHQQH QLYLQQQQQL
QRLAQQNQQQ QPTIMQQVQN QQPTQQQQQQ QQQQQQQQQQ QQLNQQQINQ IKQQQQQQQQ
QQQQHQQPVQ MPSGVTTNKQ SPQPQNTPLT PQQQQQLLAA QQSHAQAQAN QNQQLQNPKR
ISSTNNTHLM PCLPSTLYPT ATQQDINNMY WERVSSIKKY IPEIEAIIPK IIENFSQSQN
QQPQKVETYK KKFLDFVQMV KVTPETVVPP LNLDELTNAE RYLMNMYLSS LSEEEQFKKI
LGTIDDRSND NLIHFEKDLL NAFERTKRYF SDTFLNKTGG VTSKAPSILW FKNPALNNPF
TSATKCQFSD PIVPKKKQHI LLDSWTPEFK KIKKGNYEIF EKDQKIYNNL KKDLIDFTKN
DNTLLPAKFI DKDTIIISET INGGDGGNNL YIHFNQCQFS TSWKGSKKNP AMFVCSSPNI
QISSDGELFS IQPLCGLSWD RAEIIIKHYK QSLNRGDLIS KELSQVISLN RYTIESHLDL
ENAKFFIQFS TKSTLLLKSK KQQQQQQDEK INNNNNNFYN LSILLEVPNN YPYSPISYSF
PPEYQLTPYL KDLESKMNKE FENSNDLNQL NNNINNNNNN NNSFSDNTQL PTNQSIVESL
KIFEKVLKDL V
