MED15_DROME
ID MED15_DROME Reviewed; 749 AA.
AC Q9Y149;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Mediator complex subunit 15;
DE AltName: Full=dARC105;
GN Name=MED15; Synonyms=Arc105; ORFNames=CG4184;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC activated transcription of the MtnA, MtnB and MtnD genes. {ECO:0000250,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; AE014134; AAF51490.1; -; Genomic_DNA.
DR EMBL; AF145620; AAD38595.1; -; mRNA.
DR RefSeq; NP_608528.1; NM_134684.3.
DR AlphaFoldDB; Q9Y149; -.
DR SMR; Q9Y149; -.
DR BioGRID; 59479; 63.
DR IntAct; Q9Y149; 30.
DR STRING; 7227.FBpp0077722; -.
DR PaxDb; Q9Y149; -.
DR PRIDE; Q9Y149; -.
DR DNASU; 33223; -.
DR EnsemblMetazoa; FBtr0078062; FBpp0077722; FBgn0027592.
DR GeneID; 33223; -.
DR KEGG; dme:Dmel_CG4184; -.
DR CTD; 51586; -.
DR FlyBase; FBgn0027592; MED15.
DR VEuPathDB; VectorBase:FBgn0027592; -.
DR eggNOG; KOG4274; Eukaryota.
DR GeneTree; ENSGT00730000111140; -.
DR HOGENOM; CLU_011336_0_0_1; -.
DR InParanoid; Q9Y149; -.
DR OMA; CCLDDKR; -.
DR OrthoDB; 1424577at2759; -.
DR PhylomeDB; Q9Y149; -.
DR SignaLink; Q9Y149; -.
DR BioGRID-ORCS; 33223; 1 hit in 1 CRISPR screen.
DR ChiTaRS; MED15; fly.
DR GenomeRNAi; 33223; -.
DR PRO; PR:Q9Y149; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0027592; Expressed in ovary and 15 other tissues.
DR Genevisible; Q9Y149; DM.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR019087; Med15.
DR Pfam; PF09606; Med15; 2.
DR SUPFAM; SSF47040; SSF47040; 1.
PE 2: Evidence at transcript level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..749
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15"
FT /id="PRO_0000304668"
FT REGION 63..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 221..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 351..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..421
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..438
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 749 AA; 80526 MW; 42375AEB7C13A1DF CRC64;
MTEDWQSQKF RQNVISKIHD LLPPNAQDQT KNAGVMENHI FRKSRTKDEY LGLVAKLFMH
YKDMSRKSQQ QQQQQQQQGG PPPNAEMGGG QNMMQDPLNA LQNLASQGNR NPQMMPMGAG
GGAPVPGGPG TASNLLQSLN QQRPGQQQMQ PMSNIRGQMP MGAGGAGAQQ MMQVQQMQQG
GNAPGVMNVM GAGGGQNQGQ IVGNPGQQMG VGVGMPNQMV GPGPNSGPAV GGAGGPNAAP
GAGGPGPNQM QGGPMNVNAM QQMPPMQQIQ QNQLGMGMNP MMRMGQGNGM GGPQGMPGQG
MQGMPQGPHN VVGGPAGQQQ VGGAGLPPNA VQQGGMNPMG GMGVNMPPNL QQKPNMPMGQ
AGQMFPGNRG GVGVGGQQPG QPFMRSSPSP ADAQQLQQQA QLQQMQQQQQ QLVVGNQTPT
QQPPTPQMPT PNMIPSPALV PQSSPQMMQM QNSQRNIRQQ SPSASINTPG QVTGNSPFNP
QEEALYREKY KQLTKYIEPL KRMLAKISND GTNVEKMTKM SKLLEILCNP TQRVPLETLL
KCEKALEKMD LISYSGQQFG KSSNPLLEVI NTTLQSPVAN HTLYRTFRPT LELLFGTDIT
APVPAKRPRV EEKSTSFEQE VPHVLQGEIA RLDTKFKVKL DTTSQINNKA IRLICCLDDK
RLPSVPPVSV SVPEEYPWQA PDCSLAEQEY SATPFLQTVQ QALIARISKL PKNYSLSHLL
DTWEMAVRQA CSPQSKPRAV CELSTLLGV
