MED15_KLULA
ID MED15_KLULA Reviewed; 1034 AA.
AC P32257; Q6CKW6;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 3.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Mediator complex subunit 15;
DE AltName: Full=Transcription regulatory protein GAL11;
GN Name=GAL11; Synonyms=MED15; OrderedLocusNames=KLLA0F07579g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1923818; DOI=10.1093/nar/19.19.5345;
RA Dickson R.C., Hopper J.E., Mylin L.M., Gerardot C.J.;
RT "Sequence conservation in the Saccharomyces and Kluveromyces GAL11
RT transcription activators suggests functional domains.";
RL Nucleic Acids Res. 19:5345-5350(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC transcription of genes encoding galactose-metabolizing enzymes.
CC Essential for normal growth on nonfermentable carbon sources, for
CC sporulation and mating. {ECO:0000250, ECO:0000269|PubMed:1923818}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- MISCELLANEOUS: GAL11 lacks a DNA-domain, it probably complexes with
CC GAL4 that has the capacity to bind DNA. Association between GAL11 and
CC GAL4 may serve to expedite phosphorylation of GAL4.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA35254.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M68870; AAA35254.1; ALT_INIT; Genomic_DNA.
DR EMBL; CR382126; CAG98131.1; -; Genomic_DNA.
DR PIR; S19033; S19033.
DR RefSeq; XP_455423.1; XM_455423.1.
DR AlphaFoldDB; P32257; -.
DR SMR; P32257; -.
DR STRING; 28985.XP_455423.1; -.
DR EnsemblFungi; CAG98131; CAG98131; KLLA0_F07579g.
DR GeneID; 2894954; -.
DR KEGG; kla:KLLA0_F07579g; -.
DR eggNOG; ENOG502QVXD; Eukaryota.
DR HOGENOM; CLU_009962_0_0_1; -.
DR InParanoid; P32257; -.
DR OMA; KYWESMK; -.
DR Proteomes; UP000000598; Chromosome F.
DR GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR GO; GO:0001095; F:TFIIE-class transcription factor complex binding; IEA:EnsemblFungi.
DR GO; GO:0001097; F:TFIIH-class transcription factor complex binding; IEA:EnsemblFungi.
DR GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR GO; GO:2000219; P:positive regulation of invasive growth in response to glucose limitation; IEA:EnsemblFungi.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0070202; P:regulation of establishment of protein localization to chromosome; IEA:EnsemblFungi.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR CDD; cd12191; gal11_coact; 1.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR033789; Gal11_coact.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR036546; MED15_KIX.
DR InterPro; IPR008626; Mediator_Med15_fun.
DR Pfam; PF18535; Gal11_ABD1; 1.
DR Pfam; PF16987; KIX_2; 1.
DR Pfam; PF05397; Med15_fungi; 1.
DR SUPFAM; SSF47040; SSF47040; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1034
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15"
FT /id="PRO_0000087425"
FT REGION 101..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 413..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 655..682
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 712..804
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 951..1020
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..442
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 733..802
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 997..1020
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1034 AA; 117855 MW; A94BF2746DBC80D5 CRC64;
MDDWRSTIST QERSQFVTEL AQILADMSRI NGGEKANFNL EKLKKSAEQF ESSLYASCNS
KDLYLDAMRK RIAAMDSAKR KSIEAQRQKL AQAQAQAQAV QRQQQQQQQQ HHMQQQGSGQ
QQANGIPANI NAQMFLNQQA QARQQAQRQI RSTLTGQQQQ QPMPQQPQPQ QQQPNMMRPQ
LTLQQQQQLA NELKVTEIPR ELLQKIPNLP PGITTWPQIT FWAQKNRPSQ NDLIITKKVY
QLHQQLLNKS KLQQSNVNRM NTSVQQPGMG VQQSPNQRMN MNQVPQQQQQ VRQQQQQQQQ
QQQQQHKQQP QHQEPPAVLG RLNQVFTQAE QKMLYEAGKK MIENLQRSGK LPPNLDSQQQ
LLYIKKYMNQ MVLKKMQQLG IPIPMPGSAQ QQSQLLQQQQ QQQQQQQQQQ RQQQIPQVQQ
APLQQPQVQP PQSQQAQSRR QAMGMKPTPV IPNAGVMKPQ PQQTPQNIQQ PMMQQQSSPP
PQQQQQQQQQ SKISVPRPTE QDMLALKKLN AEVTKNPVKL NDITNRLTND QKQQIRSKLH
ANQQLFSSVE SFIPTLYMLT RNEEHIRQLL QIRMLTREIM EKAVRGVFLV EPNVVDKVIF
RYQKYYEYTK EQLLRRQQQL MTMRQMQNNN PNLTANDLLN QQQLLQNRKL NLAMQSQTQE
PPQIAAQQQQ PQQPISGVNN NSNMGIVLDS SLNATSRSGT NTMEFLNSPE FSAISPAVPS
PNKDKKNPAT RAVKGKKNSQ SGIPTSNPQS NSNASVVNSR TATPTVIPGS SPMFNNKSLA
SGQQNSPSPK TMINSPPQQD NPYKNDELAL KKMAIRTAEL MSRYKHRKEV FVMSSIDLFL
STFADCLDIK DDAVDLVHKT PQPILDQING TGKKKLSKAA QKARDQDPVE ISVRNNKLLM
PSKSEKTLRS FKIPIADITA CFKPFADPKQ LILNSTPKHD EKKRKFDDLE ISPTDSSSEL
MSESKKVKFD SPDDMFLNDP SSVQETKPLV PSELGMYSIN SKPSIPSSAG NMPAPNESTD
SGMNIWDWNY WESL
