MED15_XENLA
ID MED15_XENLA Reviewed; 777 AA.
AC Q6NS15; Q8JI77;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15;
DE AltName: Full=Activator-recruited cofactor 105 kDa component;
DE Short=ARC105;
DE Short=xARC105;
DE AltName: Full=Mediator complex subunit 15;
GN Name=med15; Synonyms=arc105;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH SMAD2;
RP SMAD3 AND SMAD4, AND DEVELOPMENTAL STAGE.
RX PubMed=12167862; DOI=10.1038/nature00969;
RA Kato Y., Habas R., Katsuyama Y., Naeaer A.M., He X.;
RT "A component of the ARC/Mediator complex required for TGF beta/Nodal
RT signalling.";
RL Nature 418:641-646(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for cholesterol-
CC dependent gene regulation (By similarity). Positively regulates the
CC Nodal signaling pathway. {ECO:0000250, ECO:0000269|PubMed:12167862}.
CC -!- SUBUNIT: Component of the Mediator complex. Interacts with srebf1 and
CC srebf2 (By similarity). Interacts with smad2, smad3 and smad4.
CC {ECO:0000250, ECO:0000269|PubMed:12167862}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NS15-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NS15-2; Sequence=VSP_028074;
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and throughout embryogenesis.
CC {ECO:0000269|PubMed:12167862}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; AF378334; AAM83255.1; -; mRNA.
DR EMBL; BC070536; AAH70536.1; -; mRNA.
DR RefSeq; NP_001082292.1; NM_001088823.1. [Q6NS15-1]
DR AlphaFoldDB; Q6NS15; -.
DR SMR; Q6NS15; -.
DR BioGRID; 99720; 3.
DR DNASU; 398388; -.
DR GeneID; 398388; -.
DR KEGG; xla:398388; -.
DR CTD; 398388; -.
DR Xenbase; XB-GENE-1010511; med15.L.
DR Proteomes; UP000186698; Chromosome 1L.
DR Bgee; 398388; Expressed in testis and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR019087; Med15.
DR Pfam; PF09606; Med15; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..777
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15"
FT /id="PRO_0000304665"
FT REGION 120..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 431..450
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..480
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..520
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12167862"
FT /id="VSP_028074"
FT CONFLICT 190
FT /note="V -> A (in Ref. 1; AAM83255)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 777 AA; 86686 MW; 495525A7030A812B CRC64;
MDADSDWRSA PFRQKLVSQI DEAMRKAGVA HTKSSKDMES HVFLKAKTRE EYLSLVARLI
IHFRDILNKK SQAAATDPMN ALQNLTGTAV VGGQGMGMGV RPQGAQMGAM SGMAQMAQQM
NLPGQPQPGA SGMAPHGITG VAAGNQATQL QLQQIAQHQQ QQFQQQQQQA ALQHQQQQFQ
VAQQQQFQVV AVAAAQQQQQ QQQQQQQQQQ QVQQQQQQLQ AAAQQQHILK LQLQQQQNQQ
QQLQQQQQQQ LQRIAHLQQM QQMQQQQQQQ QQQQQQQQQQ PPPQQVMQLQ QMQQQQVAQS
QQQQLLSTQP QAPSLVAQGQ IPSQVMPVTL TPQQQQQLKI LQQVRAQQQQ QAQHQAQQQA
QQQAHQQAQQ QAQQQAQQQA AQQQAQQAAQ QAQQQAQQAA QQQAAQAHLA AGQVTQNSIP
VMSSPSPVQQ VQTPQPMPPP PQPSPQPSQP MSQPNSNVSS GPAPSPSSFM PSPSPQPSQS
PASARTPQNF SVPSPGPLNT PGNPNSVMSP ASNNQSEEQQ YLDKLKQLSK YIEPLRRMIN
KIDKNEERKK DLSKMKSLLD ILTDPNKRCP LKTLQKCEIA LEKLKNDMAV PTPPPPTVPS
TKQQYLCQPL LDAVLANIRS PVFNHSLYRT FMPAMTAIHG QPIASQLVVP RKRKFEEDER
QSIPNVLQGE VAKLHSKFLV NLDPSHCSNN GTVYLICKLD DKNLPSVPPL QLSVPADYPD
QSPLWMDNPR DYEANPFLQS VYRYMTSKLL QLPDKHSLTA LLNTWAQSIR QACLSAA
