ID   MED16_AEDAE             Reviewed;         820 AA.
AC   Q16K67;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=MED16; ORFNames=AAEL013087;
OS   Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Aedini; Aedes; Stegomyia.
OX   NCBI_TaxID=7159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LVPib12;
RX   PubMed=17510324; DOI=10.1126/science.1138878;
RA   Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA   Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA   Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA   Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA   Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA   Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA   El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA   Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA   Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA   Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA   Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA   Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA   Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA   Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA   Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA   Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA   Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT   "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL   Science 316:1718-1723(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
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DR   EMBL; CH477974; EAT34700.1; -; Genomic_DNA.
DR   RefSeq; XP_001663311.1; XM_001663261.1.
DR   AlphaFoldDB; Q16K67; -.
DR   SMR; Q16K67; -.
DR   STRING; 7159.AAEL013087-PA; -.
DR   GeneID; 5577215; -.
DR   KEGG; aag:5577215; -.
DR   VEuPathDB; VectorBase:AAEL013087; -.
DR   eggNOG; ENOG502QQ3H; Eukaryota.
DR   HOGENOM; CLU_018773_0_0_1; -.
DR   InParanoid; Q16K67; -.
DR   OMA; DSHAQLH; -.
DR   OrthoDB; 493637at2759; -.
DR   PhylomeDB; Q16K67; -.
DR   Proteomes; UP000008820; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   InterPro; IPR021665; Mediator_Med16.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR13224; PTHR13224; 1.
DR   Pfam; PF11635; Med16; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; WD repeat.
FT   CHAIN           1..820
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307621"
FT   REPEAT          74..114
FT                   /note="WD 1"
FT   REPEAT          621..665
FT                   /note="WD 2"
FT   REPEAT          757..811
FT                   /note="WD 3"
SQ   SEQUENCE   820 AA;  92495 MW;  79E1F965D5407376 CRC64;
     MDLLYTTVRK GNANTFSFTE TQHNFEFLCN TSSCNVLAFT TTNELTEPSP IQGWGFHIYV
     VDLNAPWFPY RVTSNKYPIS VLQWDVLGRY LLVGDRNGHV QIFVQKDNLL SEWKSVYSVK
     FPGENIIAAA FFHNGRKLNL QTDKKDQYLY LEKFQKVKFA ASVRQFGGVP VEGVLIVSAT
     GMLGAFVIPP ESNANIQKLS EPIKLVPVTE SLAVTRNYYT TADICYGKNG YFRVAVGNGD
     SKSANSNMIQ CFRVAVKQRE ETLEINSTAL PSFFLAEGMG KDLKELRVAH VQWVFSEDAD
     SLIVASNHTG GGCFVEQWEL TEESTPIHKL FQSNKNEPFK TLLWVSKSQY MYTSKVIKIC
     TTKLNFLSSS FVYLAMSDNS IHCLQRDTLK RLTCTNIVSI RDPNDHSSKQ IKLGLKIGAI
     DVTHLGHLFV TLDTFGQLYV YRCNFMCQDQ LGTPALIVQT VNLLEYCLVT GLDSLDILLT
     LKTQILENVL ERLTENFHRQ PPNVQQYYYV NFLTMKIALY RLSISGQQKA HDLSNLLILH
     SILIAFKSLL RPSDLTSHDK GPAENLAMVL SESVPDVDKV LLNVEAKEFT MERPTLQSLQ
     QLIQWVADLA LNILAKLPES RSFMSNKSQG YDISKDIIAL NSIRELLVMI RIWGLLNPQC
     LPVFSRSADN LDILGTLFRL LTKLSLNPNE PDDLLLDECC LLPNQVLIPQ LQYVPSRTMI
     ASPLLPHVTL PVMCDYGVEN ESLKFCPEVP IVEGGLSNDN VIDSVMYLQL GRRPPSLRRC
     TRCGSCSSVV SVAKTAAMKA WEQRWIDKCR CNGFWRLEVA