MED16_ARATH
ID MED16_ARATH Reviewed; 1278 AA.
AC F4JGZ1; F4JGZ2; Q9M0Y7;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE AltName: Full=Protein SENSITIVE TO FREEZING 6;
GN Name=MED16; Synonyms=MED16_1, SFR6; OrderedLocusNames=At4g04920;
GN ORFNames=T1J1.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), FUNCTION, INDUCTION,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19067974; DOI=10.1111/j.1365-313x.2008.03763.x;
RA Knight H., Mugford S.G., Ulker B., Gao D., Thorlby G., Knight M.R.;
RT "Identification of SFR6, a key component in cold acclimation acting post-
RT translationally on CBF function.";
RL Plant J. 58:97-108(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION.
RX PubMed=12753580; DOI=10.1046/j.1365-313x.2003.01734.x;
RA Boyce J.M., Knight H., Deyholos M., Openshaw M.R., Galbraith D.W.,
RA Warren G., Knight M.R.;
RT "The sfr6 mutant of Arabidopsis is defective in transcriptional activation
RT via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress.";
RL Plant J. 34:395-406(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP FUNCTION.
RX PubMed=18614706; DOI=10.1104/pp.108.123901;
RA Knight H., Thomson A.J., McWatters H.G.;
RT "Sensitive to freezing6 integrates cellular and environmental inputs to the
RT plant circadian clock.";
RL Plant Physiol. 148:293-303(2008).
RN [7]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [8]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=22494141; DOI=10.1111/j.1469-8137.2012.04138.x;
RA Wathugala D.L., Hemsley P.A., Moffat C.S., Cremelie P., Knight M.R.,
RA Knight H.;
RT "The Mediator subunit SFR6/MED16 controls defence gene expression mediated
RT by salicylic acid and jasmonate responsive pathways.";
RL New Phytol. 195:217-230(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors. Involved in the regulation of the circadian
CC clock, in the control of flowering time, in freezing- and osmotic-
CC stress tolerance and in both salicylic acid- and jasmonate-mediated
CC defense gene expression. {ECO:0000269|PubMed:12753580,
CC ECO:0000269|PubMed:18614706, ECO:0000269|PubMed:19067974,
CC ECO:0000269|PubMed:22494141}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19067974}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4JGZ1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JGZ1-2; Sequence=VSP_044033;
CC -!- INDUCTION: Not up- or down-regulated by cold.
CC {ECO:0000269|PubMed:19067974}.
CC -!- DISRUPTION PHENOTYPE: Late flowering in long days. Very low expression
CC of cold-responsive (COR) genes. {ECO:0000269|PubMed:19067974}.
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 16 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB81034.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL161502; CAB81034.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82441.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82442.1; -; Genomic_DNA.
DR PIR; H85061; H85061.
DR RefSeq; NP_001190676.1; NM_001203747.1. [F4JGZ1-2]
DR RefSeq; NP_192401.5; NM_116730.5. [F4JGZ1-1]
DR AlphaFoldDB; F4JGZ1; -.
DR BioGRID; 11142; 21.
DR STRING; 3702.AT4G04920.1; -.
DR PaxDb; F4JGZ1; -.
DR PRIDE; F4JGZ1; -.
DR ProteomicsDB; 250842; -. [F4JGZ1-1]
DR EnsemblPlants; AT4G04920.1; AT4G04920.1; AT4G04920. [F4JGZ1-1]
DR EnsemblPlants; AT4G04920.2; AT4G04920.2; AT4G04920. [F4JGZ1-2]
DR GeneID; 825831; -.
DR Gramene; AT4G04920.1; AT4G04920.1; AT4G04920. [F4JGZ1-1]
DR Gramene; AT4G04920.2; AT4G04920.2; AT4G04920. [F4JGZ1-2]
DR KEGG; ath:AT4G04920; -.
DR Araport; AT4G04920; -.
DR TAIR; locus:2135383; AT4G04920.
DR eggNOG; ENOG502QU1U; Eukaryota.
DR InParanoid; F4JGZ1; -.
DR OrthoDB; 80000at2759; -.
DR PRO; PR:F4JGZ1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JGZ1; baseline and differential.
DR Genevisible; F4JGZ1; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IDA:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0032922; P:circadian regulation of gene expression; IMP:TAIR.
DR GO; GO:2001011; P:positive regulation of plant-type cell wall cellulose biosynthetic process; IMP:TAIR.
DR GO; GO:1901672; P:positive regulation of systemic acquired resistance; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:TAIR.
DR GO; GO:1902066; P:regulation of cell wall pectin metabolic process; IGI:TAIR.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:TAIR.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0048586; P:regulation of long-day photoperiodism, flowering; IMP:TAIR.
DR GO; GO:2001009; P:regulation of plant-type cell wall cellulose biosynthetic process; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IMP:TAIR.
DR GO; GO:0048364; P:root development; IGI:TAIR.
DR GO; GO:0010091; P:trichome branching; IMP:TAIR.
DR GO; GO:1905499; P:trichome papilla formation; IMP:TAIR.
DR InterPro; IPR038836; MED16.
DR PANTHER; PTHR35130; PTHR35130; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Jasmonic acid signaling pathway; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1278
FT /note="Mediator of RNA polymerase II transcription subunit
FT 16"
FT /id="PRO_0000418351"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 839..861
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 864..874
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19067974"
FT /id="VSP_044033"
SQ SEQUENCE 1278 AA; 138187 MW; D612D8F90B747D42 CRC64;
MNQQNPEEEV SLVNNSGGGG IIEAPAIVEE KEEEGLQQKQ EETIESTDPI LVVVEEKLLE
KSVDGEKEDD NSSSSNMEID PVSPATVFCV KLKQPNSNLL HKMSVPELCR NFSAVAWCGK
LNAIACASET CARIPSSKAN TPFWIPIHIL IPERPTECAV FNVVADSPRD SVQFIEWSPT
SCPRALLIAN FHGRITIWTQ PTQGSANLVH DATSWQCEHE WRQDIAVVTK WLTGASPYRW
LSSKPSSGTN AKSTFEEKFL SQSSESSARW PNFLCVCSVF SSGSVQIHWS QWPSNQGSTA
PKWFSTKKGL LGAGPSGIMA ADAIITDSGA MHVAGVPIVN PSTIVVWEVT PGPGNGLQAT
PKISTGSRVP PSLSSSSWTG FAPLAAYLFS WQEYLISEIK QGKKPSDQDS SDAISLSCSP
VSNFSAYVSP EAAAQSAATT TWGSGVTAVA FDPTRGGSVI AVVIVEGQYM SPYDPDEGPS
ITGWRVQRWE SSVQPVVLHQ IFGNPTSNFG GQVPTQTVWV SRVDMSIPPT KDFKNHQVAA
AGPSVDAPKE PDSGDEKANK VVFDPFDLPS DIRTLARIVY SAHGGEIAIA FLRGGVHIFS
GPTFSPVENY QINVGSAIAA PAFSPTSCCS ASVWHDAAKD CAMLKIIRVL PPALPRNQSK
VDQSTWERAI AERFWWSLLV GVDWWDAVGC TQSAAEDGIV SLNSVIAVMD ADFHSLPSTQ
HRQQYGPNLD RIKCRLLEGT NAQEVRAMVL DMQARLLLDM LGKGIESALV NPSALVFEPW
RVDGETITGI NPEAMAVDPA LVSSIQAYVD AVLDLASHFI TRLRRYASFC RTLASHAASA
GTGSNRNNVT SPTQNASSPA TPQVFPDKSL YLAVGQPTTT TTTTATTNSS GSSHVQAWMQ
GAIAKISSSN DGSNSTASPI SGSPTFMPIS INTGTFPGTP AVRLIGDCHF LHRLCQLLLF
CFLQRSSRFP QRNADVSSQK LQTGATSKLE EVNSAKPTPA LNRIEDAQGF RGAQLGTGVK
GIDENSARTT KMGSGNAGQG YTYEEVRVLF HILMDLCKRT SGLAHPLPGS QVGSGNIQVR
LHYIDGNYTV LPEVVEAALG PHMQNMPRPR GADAAGLLLR ELELHPPSEE WHRRNLFGGP
GSEPEDMILT DDVSKLSNSL DLPDTNFSGI CDGYNRVHSL WPRKRRMSER DAAFGSNTSV
GLGAYLGIMG SRRDVVTATW KTGLEGVWYK CIRCLRQTSA FASPGATKQP NPNERETWWT
SRWVYCCPMC GGTWVRVV
