ID   MED16_ASHGO             Reviewed;         938 AA.
AC   Q75E72;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=SIN4; Synonyms=MED16; OrderedLocusNames=ABL202W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 282.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016815; AAS50569.2; -; Genomic_DNA.
DR   RefSeq; NP_982745.2; NM_208098.2.
DR   AlphaFoldDB; Q75E72; -.
DR   STRING; 33169.AAS50569; -.
DR   PRIDE; Q75E72; -.
DR   EnsemblFungi; AAS50569; AAS50569; AGOS_ABL202W.
DR   GeneID; 4618824; -.
DR   KEGG; ago:AGOS_ABL202W; -.
DR   eggNOG; ENOG502QWAC; Eukaryota.
DR   HOGENOM; CLU_311703_0_0_1; -.
DR   InParanoid; Q75E72; -.
DR   OMA; WYQFSNS; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0070202; P:regulation of establishment of protein localization to chromosome; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR021665; Mediator_Med16.
DR   Pfam; PF11635; Med16; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..938
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307623"
SQ   SEQUENCE   938 AA;  105826 MW;  A061ADF074025833 CRC64;
     MHLEHLMSST TAVSWSKIGL IAYGDAQSQD GNLCITFLET VNGSNWRFHP PKKYVIHPQL
     HEDQSSNGNP KSPLFFHDLR SMHWNNWGLL NGELLAVCDE LGNMTVLAAG QGLNGNGAYD
     RLSVLFQDNI FKIHNQVLPL ESVPKKDATP KVERKHTKKE YYSTILDFQW IGNQKPSVAQ
     VAAQRDRTNN IFKNQMHQCP PYGVFHPASI KSACIAIRRN GYIDLWYQFS NTLDFKKISL
     QLSKDKESEW LQYAQIAHTD KEQCFLIGVF SNVAKSFAFY ELLINWNVNT TNQAMFHDPK
     LTLRHILRVR PDALGPNGEL LKLENFHVIS KTALPGTRPE ILISYNILGT ERSLVRRFQM
     VLSNPDMVFL SSLGLAITAL HNNHSVLRYS MKHVQDLTFD SKIMDIQSHS LDALVCFRLH
     NGRFQLYNRH TWAVESETAD AKQIGGYTKD TIISIFGSGF NYPLLPPADA IEWCAISPSS
     GGIILKLKCK PQPTFYALEQ SVLTDPSRDI VHATAFAFEF VRFNNMIHSG EDLAIAAKTH
     VFRLQRLSEE RAVNFIASVI GAILSLYGIH FDGPKEILEK LLQSKAIQKI FLLQMELGSH
     LKNKNVYSMA YASMKLRSIN LAMNGVARNV HAMIQHTAVV NSLPNGRAFQ FAFSKQDLIY
     SLIPSVNWFV SFVTFLTQQL IMLVNNPMDN THSLVLGILS CITTRHLMLK VILELKNMIG
     LITKFPETTY TVLNESSRFL RKALGDSPVN LEKFEVFLNE INNKFLSLLD DHGAQSMDRE
     PSFMVKAEIP PALGHVREFL LSFAGSALLA QTNLAEVFFA STHNLRIFDH QHFHPSVANL
     LQPPEKGLVV DDAILPDACR GSSSFSPLDY DDISSEWVDM SALVRIKRCV RCGCVTRAGN
     PVAKNNTILE TSIVTKRWTA LYSRYCQCTG LLYELDTP