ID   MED16_CANAL             Reviewed;        1053 AA.
AC   Q59PP6; A0A1D8PRC3; Q3MP22; Q59PM8;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 2.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=SIN4; Synonyms=MED16; OrderedLocusNames=CAALFM_C703320CA;
GN   ORFNames=CaJ7.0384, CaO19.1343, CaO19.8923;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15937140; DOI=10.1534/genetics.104.034652;
RA   Chibana H., Oka N., Nakayama H., Aoyama T., Magee B.B., Magee P.T.,
RA   Mikami Y.;
RT   "Sequence finishing and gene mapping for Candida albicans chromosome 7 and
RT   syntenic analysis against the Saccharomyces cerevisiae genome.";
RL   Genetics 170:1525-1537(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
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DR   EMBL; AP006852; BAE44838.1; -; Genomic_DNA.
DR   EMBL; CP017629; AOW30676.1; -; Genomic_DNA.
DR   RefSeq; XP_711683.2; XM_706591.2.
DR   AlphaFoldDB; Q59PP6; -.
DR   BioGRID; 1229818; 2.
DR   STRING; 237561.Q59PP6; -.
DR   PRIDE; Q59PP6; -.
DR   GeneID; 3646725; -.
DR   KEGG; cal:CAALFM_C703320CA; -.
DR   CGD; CAL0000174522; MED16.
DR   eggNOG; ENOG502QWAC; Eukaryota.
DR   HOGENOM; CLU_013428_0_0_1; -.
DR   InParanoid; Q59PP6; -.
DR   OrthoDB; 209987at2759; -.
DR   Proteomes; UP000000559; Chromosome 7.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR021665; Mediator_Med16.
DR   PANTHER; PTHR13224; PTHR13224; 1.
DR   Pfam; PF11635; Med16; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1053
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307626"
FT   REGION          139..170
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        149..170
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        52
FT                   /note="Missing (in Ref. 1; BAE44838)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194
FT                   /note="V -> S (in Ref. 1; BAE44838)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        495
FT                   /note="S -> F (in Ref. 1; BAE44838)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        512
FT                   /note="K -> W (in Ref. 1; BAE44838)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1053 AA;  117759 MW;  46B8BFB9AC3C6FFC CRC64;
     MATQTDKQTI GRHLEQVPKA SNLISWSRNG FIAYIPPIIT TTTTTPTTTT TTNNNKSNLL
     LTYIKNSDGK KWQLASPEPI NIKLENNFLP QLSLVSWGSL NTDLAVSDIY GNFYILLAGV
     GLLDPNHIPS TITTTSTIKT EGNTEKNKDT KQIGNGSGTN GHGDSPINTP SFELTSYNHM
     EMIYRDIINP DINVAVNPGA SIVAFKWLNI EKPQIVNKAA TRLAENPTTT SSNSSSSIYG
     YGINQYQPYG VCHPIPTKQA CVALRKNGQF ILFYQGEHKV EYHKICCNLT DNISIIEKAS
     IGFNNDKQII VTAWDSLSND INVYSIDINW GFLVESAKRQ KLDQHYHTPK EAQKPPRLTL
     KKLHQMKPIQ CFKEEDGESQ SQSVVPEMGK LKVEELSSID IISSNPDPKS GLSILITYGS
     SIIYRYALEK SDSSNTTSGG ETSKNPISQA FYNLGVEKKI DWKGDDSSVR LVFKDKLMRR
     GQIESIINGF LDLSSLIIYK DGTVDVVDTT SKQIVNNSKN NNSVTGDEME LDNNDYPPKL
     ISNLFDQGFQ FPKISHKNRL VLAISPTMSS IVYTEIYGET VNLQLKPLER IDNFGTNSHD
     LYYTSVAFAH RYAFALYTST CSDDLLLLIQ SEINRIKDAV DGDTITRNKL GKQLCDSVII
     ECHKAINFHL DTITKESLDK LLSNNASLQK LLSLQLILGE FQQSSHFPNN YVVPDIAWIV
     LNLRSASLGI MFTLSSIYRQ VSKKKPSEDT LQDSITRGEC IMSIIGNFKW LIDLLVYLNQ
     ELLQLIYVKN NFLNNGNTTT SKLTLSNSIV LPLILNKVSR LFLMYAISAM GRTHEILKKL
     HKDLTEANKL FAPMKESLNR YFSISNNSPI TVNLFESYLR ECDALLNKEV PQKILTANNS
     INGNVNGNSG TSTNKPYSAL KFEQKLLIKG IDVNDDNDSS TKISNTIIEE LSNMILDRYS
     ISISRETKLS ELMFYDTDWL NIGINKKEVP SYTIDALRKL IISDSSTSTS LAKGEKLRVC
     TRCRAVSLVG DVTGLWTMVF QRTCMCGNAW VNV