ID   MED16_DICDI             Reviewed;        1284 AA.
AC   Q54X75;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 2.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Putative mediator complex subunit 16;
GN   Name=med16; ORFNames=DDB_G0279157;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000027; EAL67878.2; -; Genomic_DNA.
DR   RefSeq; XP_641853.2; XM_636761.2.
DR   AlphaFoldDB; Q54X75; -.
DR   STRING; 44689.DDB0266902; -.
DR   PaxDb; Q54X75; -.
DR   EnsemblProtists; EAL67878; EAL67878; DDB_G0279157.
DR   GeneID; 8621898; -.
DR   KEGG; ddi:DDB_G0279157; -.
DR   dictyBase; DDB_G0279157; med16.
DR   eggNOG; ENOG502RDPH; Eukaryota.
DR   HOGENOM; CLU_262861_0_0_1; -.
DR   InParanoid; Q54X75; -.
DR   OMA; MEVAFVD; -.
DR   PRO; PR:Q54X75; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; ISS:dictyBase.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR021665; Mediator_Med16.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR13224; PTHR13224; 2.
DR   SUPFAM; SSF50998; SSF50998; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; WD repeat.
FT   CHAIN           1..1284
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 16"
FT                   /id="PRO_0000388655"
FT   REPEAT          188..229
FT                   /note="WD 1"
FT   REPEAT          548..587
FT                   /note="WD 2"
FT   REGION          55..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          267..288
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          608..687
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1085..1121
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        608..637
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        644..687
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1284 AA;  147190 MW;  D815CB0A8C5603BB CRC64;
     MIESVWSIKI MEGVSEDFYD SGPTFQYPNP LSSIYDKSMN IDLMNHYHPS TSLITNSNNP
     FYESLSDTSS DDLNNEDNSN SNNNQNNNNN NNNNNNNNNQ NSNNKLLGIE KQLQSINLLT
     NKVDWSDYHQ QVYHGCSNEN SQLISWSVDN QIAFTMNFNK NQFIKQEQQQ IYITRVEYPH
     EYVVIKTSHT NSIKCLEWST QSPSNLLLSF DQSDKLCLWK TKDNCSDDWY CLHIFPFQYL
     LLSKWLPLRS TYTPIFDPFN LNNTNNNNNN TTNINNNNNN NSNNSNTSTN TFKSPFESLI
     TDNHIDNSFQ TFISITKDGK IYIGYYGIVE SCWKYIIGQI PLPFYHTIDC VDIFPTQEPD
     SLLVTIYSKN IGLALIVYHI NYNIHTSEVT VTLKDKISMS NIIEQQQQQQ QQQQQQQQQQ
     QQQQQQQQQQ QQQQQQQQQQ QQQQDVDIRS LSFRISSIQF DNSMSSLIVL MKGIDSSAIY
     RLEKKLISID SPQLSIFELL SGTAPNETNQ EIEEWTCTAK RLFYTPNNNN SNNNSNSNNN
     NNSGNNFNHY STISSIYFSS TTGFLWLTHS NGSIEIVDRL TFESISTLKE SEKPIDNIII
     SSNLNNENSE QSIMNSPTIS GSTSPILQST KANSPHSIKD DSMVIDPSNP TSTSTTTSST
     TSQIQEQDNN NNNNNNNNFL PSTKNTTLSI TSSPNGGLIA CLNSDLKVTL YSLPIKSIGT
     SPDNQKVVSK WLAQLFQNSM LKSNDWWDIL ILLKIYSTSP DTRTTFHHTI IHLSSDFSCL
     PLTFQQTYRH CFDAIKASIY RIVSGMEVAF VDSQAKSFIY YLCDTLKSSF SPLLMQPSQQ
     SDGTTNQQTL KERENIIILI DWAIEFSQFF LKNCYYFSFV YAKKGDISVK IPESMLDLPI
     KHYFPFYYSE TKGNFHFPII SFLCDFNLLF VIFELMNISK LFKEKTSTSQ SKVKFKKSKE
     IDFFLEYFTK IIKVLCKEVV SSSSSSNNNN PDEINNTLGL IDTYLSTFYG FLSEDKLDQV
     ITPPAIECKG SHMNSIIAIM KKFDSIIPSE VIADTNDISI NISYSILDLD PPLDGFLDQN
     PITSSSLSTI QQQQQQQQLG NSSNNNNYNN NNNNDTSGNG SVIFKYSPKS TEYMGIVHDI
     ITRIPIAPRI DKPYKKCIRC HRITVMLRTS MFWSDRWSMA CPICGGKWKS YQSSSSSYSS
     SSNTHDIANN FNSNFYNNNY SSNNFNSNYY NSNNNNNYNN NNYNNNKYYF NNSNNNKINK
     HLNKTNKHLN NIHRHHNHIL INRT