ID   MED16_DROME             Reviewed;         818 AA.
AC   Q9W278; Q8MT57; Q9GYW8;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 2.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=MED16; Synonyms=Trap95; ORFNames=CG5465;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Southworth J.W., Kennison J.A.;
RT   "Transcriptional coactivators in Drosophila.";
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RA   Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA   Celniker S.E.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   FUNCTION, AND INTERACTION WITH DIF.
RX   PubMed=15297616; DOI=10.1073/pnas.0401985101;
RA   Kim T.W., Kwon Y.-J., Kim J.M., Song Y.-H., Kim S.N., Kim Y.-J.;
RT   "MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and
RT   heat-shock-induced transcriptional activators.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12153-12158(2004).
RN   [7]
RP   FUNCTION.
RX   PubMed=16751183; DOI=10.1101/gad.1418806;
RA   Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT   "Coactivator cross-talk specifies transcriptional output.";
RL   Genes Dev. 20:1458-1469(2006).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). Required for
CC       activated transcription of the MtnA, MtnB and MtnD genes. Required for
CC       transcriptional activation in response to lipopolysacchardie (LPS).
CC       {ECO:0000250, ECO:0000269|PubMed:15297616,
CC       ECO:0000269|PubMed:16751183}.
CC   -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC       with Dif. {ECO:0000250, ECO:0000269|PubMed:15297616}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM48398.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF289994; AAG02459.1; -; mRNA.
DR   EMBL; AE013599; AAF46815.2; -; Genomic_DNA.
DR   EMBL; AY118369; AAM48398.1; ALT_FRAME; mRNA.
DR   EMBL; BT025233; ABF17924.1; -; mRNA.
DR   RefSeq; NP_611650.2; NM_137806.4.
DR   AlphaFoldDB; Q9W278; -.
DR   SMR; Q9W278; -.
DR   BioGRID; 63152; 31.
DR   IntAct; Q9W278; 1.
DR   STRING; 7227.FBpp0071684; -.
DR   PaxDb; Q9W278; -.
DR   PRIDE; Q9W278; -.
DR   DNASU; 37535; -.
DR   EnsemblMetazoa; FBtr0071770; FBpp0071684; FBgn0034707.
DR   GeneID; 37535; -.
DR   KEGG; dme:Dmel_CG5465; -.
DR   CTD; 10025; -.
DR   FlyBase; FBgn0034707; MED16.
DR   VEuPathDB; VectorBase:FBgn0034707; -.
DR   eggNOG; ENOG502QQ3H; Eukaryota.
DR   GeneTree; ENSGT00390000003821; -.
DR   HOGENOM; CLU_018773_0_0_1; -.
DR   InParanoid; Q9W278; -.
DR   OMA; DSHAQLH; -.
DR   OrthoDB; 493637at2759; -.
DR   PhylomeDB; Q9W278; -.
DR   BioGRID-ORCS; 37535; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 37535; -.
DR   PRO; PR:Q9W278; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0034707; Expressed in saliva-secreting gland and 14 other tissues.
DR   Genevisible; Q9W278; DM.
DR   GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR   GO; GO:0003712; F:transcription coregulator activity; IDA:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   GO; GO:0060260; P:regulation of transcription initiation from RNA polymerase II promoter; ISS:FlyBase.
DR   GO; GO:0009408; P:response to heat; IMP:UniProtKB.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IMP:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR021665; Mediator_Med16.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR13224; PTHR13224; 1.
DR   Pfam; PF11635; Med16; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   1: Evidence at protein level;
KW   Activator; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; WD repeat.
FT   CHAIN           1..818
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307622"
FT   REPEAT          86..125
FT                   /note="WD 1"
FT   REPEAT          296..342
FT                   /note="WD 2"
FT   REPEAT          615..666
FT                   /note="WD 3"
FT   REGION          175..524
FT                   /note="Interaction with Dif"
FT   CONFLICT        191
FT                   /note="T -> P (in Ref. 1; AAG02459)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   818 AA;  89979 MW;  9AC366D99948F4D5 CRC64;
     MTILYKVEGK EFSKDSVFPH KKVLCSVSAR NIVAFSALQS AIFPASHGGD AGDGSLPGAG
     LAVGASNSHV YVCDIVTPWE YYKVCSSKSL ISVLQWSPNG EQLLLGYVGG RVEIWQPRNQ
     SINLWVLQYY ATVPSEDIIE AQFFQNGKGV IFNALKKDHT YYAEKFERVE QQRPTLSGFG
     GVASEGCVLL TSSGLLAAFS LPAIQKTSAA GADGPAHEVV ELTPALHSIG ISRSFIEHCS
     MTPSPSGALN IAFSCGWQQQ LVQCFKVSLS LEGELGLEQH LAIKSESQTS IFLGPLDGRR
     ISHLKWTRIA NEDVIFIAYA CPDGAGSQLE QWTLTRRHQS VHALLQGGGG ANNKPNEFVQ
     SESWEQVGKV QLNASLADIS VTRLSVSTPD SCQVYAILQD NSVQVLEPNS MKQLNHTQFD
     RLSDASGAVK FVSGDLTPSS QILLIFDSHA QLYAMQAPLP KQGGSGLLLL EYCIVTGCDA
     SDVLLLNLGN LEALVEKLTD NFTRQPSFVR HFYYANFLAL KSNICRMQQQ EFDNLIILHA
     ISTTFKSLLR PSDLGFQDKG PADNLAIKLA ESIPDVDTVM LNLDAKDFTV EPVMLQSLQQ
     LIQWVTDLAL NMLHRLPEEV MKTKLSGKRP SYDISRDIVA ISSLRELLVM IRIWGLLNTQ
     CLPVYTKTMD NIDVLVILFR LLTRLAQNPA EPDEMLLDEC SLLSKQLLIP QPTKFNPTTL
     LSAQGFAAVK SGQLQFTSME EPTCLQDMET EEVVFASSVK DGVSNLQLGA RPSHIRRCAR
     CGFVFVNNAS KVAKTSALKA WFSKWLHCHC GGFWKQVH