ID   MED16_EMENI             Reviewed;         930 AA.
AC   Q5BE03; C8VSR4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=sin4; Synonyms=med16; ORFNames=AN1227;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CBF87897.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EAA65820.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AACD01000017; EAA65820.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BN001308; CBF87897.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_658831.1; XM_653739.1.
DR   AlphaFoldDB; Q5BE03; -.
DR   STRING; 162425.CADANIAP00001397; -.
DR   EnsemblFungi; EAA65820; EAA65820; AN1227.2.
DR   GeneID; 2876997; -.
DR   KEGG; ani:AN1227.2; -.
DR   eggNOG; ENOG502QQU3; Eukaryota.
DR   HOGENOM; CLU_007624_0_0_1; -.
DR   InParanoid; Q5BE03; -.
DR   OrthoDB; 209987at2759; -.
DR   Proteomes; UP000000560; Chromosome VIII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR021665; Mediator_Med16.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR13224; PTHR13224; 1.
DR   Pfam; PF11635; Med16; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..930
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307628"
SQ   SEQUENCE   930 AA;  102378 MW;  6DA9C1FE87497343 CRC64;
     MPLIMEDGIN VDDLFGESTS LELGLPTSTP TASTKGLAQR LDEMRLVGCC QKIAWSRLGC
     IASISQDSTR VNVRHLQCQQ SDGKWLLSDE TPLNQVSEVH AGHALVHLCW NDSGTELAVA
     DSSGRVSIYS IPIALNSVNV TRQAAFDPDD DGAQIVGMMW LSQYRSVHSF YQAAKVQGRW
     AYSPFRRRPI GTFHPAHKGA LLCITRSGVM KLLYQNPDGR WAEITTELKN TSYSDRLLTH
     AALVTTQAGI LVATHSACQR ISLYRVSIQW TPSQWDPTQQ KQGSQQFPTP SFRLVHMKVE
     VPYNIPSSNR DAGENPDEQL PFTSSLYCLT SLHIILPASD NSAGSTPNPW IVAVFSIPTH
     AILPHPQPQI PASVIVRWQL ETSLMTLHPR FDDVPSKKGN GQVKPKTVLR RLDDITSGRH
     VISVDQTEYG NVLAVSYDDS SIAFYDPKTM AAFDGIDDAN TVTSLAQAGF HYPLEPSALH
     MSFSPSACLI VGLDNDGQTH LRIMEHSYGA ENGHYDENKY SAAIASLTLA FCRGCGGEVN
     TDDILLILIR QLSPDAQTAF LNEVYRALSI NCNFTTEQDK LMNHPYIPRA LSIQAALGFK
     GKFRARNIAS NVPWAIIQLR QAAMIYPSFF QYNKGVQGPE PHDPDVLRLV LGNTKWALDF
     SLYILNELFD LADDFESVSS DQEAFNQKLK TTTSLPLIIL LSSMSRAFLR FICRGLRGIH
     AGYANAPLTG DSRVYYTEIC QTLDASPVRT DVYEKLLAGV DSAVKHAYTG AGFGDNERPG
     PEKELLVSSR IPPVLVPAVS TILRQTVPAM RGEMDRLAIF MGDYGWLGFG NDARSELYRR
     ERDVDIIKKT PIASRSGKGS GLGSVEAVMG LGARNGNGSQ LSRRCVRCCE VSEAMYPPRS
     VLSFKMTLKL GHLRSCICGG MWNLEGGLPG