ID   MED16_KLULA             Reviewed;         956 AA.
AC   Q6CQ49;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=SIN4; Synonyms=MED16; OrderedLocusNames=KLLA0D19767g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382124; CAH01036.1; -; Genomic_DNA.
DR   RefSeq; XP_453940.1; XM_453940.1.
DR   AlphaFoldDB; Q6CQ49; -.
DR   STRING; 28985.XP_453940.1; -.
DR   EnsemblFungi; CAH01036; CAH01036; KLLA0_D19767g.
DR   GeneID; 2893093; -.
DR   KEGG; kla:KLLA0_D19767g; -.
DR   eggNOG; ENOG502QWAC; Eukaryota.
DR   HOGENOM; CLU_311703_0_0_1; -.
DR   InParanoid; Q6CQ49; -.
DR   OMA; WYQFSNS; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0070202; P:regulation of establishment of protein localization to chromosome; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR021665; Mediator_Med16.
DR   Pfam; PF11635; Med16; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..956
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307629"
SQ   SEQUENCE   956 AA;  107961 MW;  B0F6D926C3E104DB CRC64;
     MPSVSSSRNE FPMLSSTCSS ASWSKIGLIA YPDNSTADGN LSITFLETIN GTNWKFHKPY
     NYLIHPHMYD DPKSNGSSTS NGVKQGQFFY DIDFVKWNNW SLLGGELLAV SDSVGNLTIL
     AASLNDEGNT TYESLSMLFQ DTIFKVHNKF LHLTPINSYT SADAKDNSKL ERKGTKKEYM
     TRILDFQWCG NSKPILTPLG AMKTPQTEPP FYKNHIQNCT PIGVFHPASV KHAFTAIRTN
     GMVDFWYQLS NSKDFKKISL PVNQGSRQNE FEWLENASIA HMDEDQCFLI GTFSNVSQKI
     NFYKLKVDWN VNGSTVNDPS LQLTHIYEML PETTYKASVL LKLTNMKILS RTPIPNSKPE
     ILLTYRSLNQ NQSATTFRSI VKRYQITSTS LNETFLESLS IQVPSQRITH YCLKSMGTLE
     LQNLLKEITA EDLCISTAFK YDDGHIEIYS RNNWKLQIGT ESSTTGGYDK PFIQSMFSVG
     FKYQDIPTSA NVEWSIISPS MGGTLIKFLN EPLPVFKSVL NEVITDPIRD QIHATGLALV
     FVILSHRNFS GEDITITIKT HVLRLQELDP KRALNFISML ISTIFGLYGL VLDGTKGTLD
     KALQSKAVQK ILLLQMEMGP HIIANKNIYS VGYIAMKLRN MNIAFNGVAR NVQAMIQHTS
     NISMLPNGKV FQFAFSKQDL IYSVLPSAKW IITFISFVTQ QLILKVNNVP DSDESLLISL
     FGSRIIRHLV SKVIRELTNI ANLMIKFPEI AFPILNESSQ FFRKVINDCT VNLDLFETFL
     SEFGSKINEL DQNTVIVQQK YEAEFLIKTE VPDSLPEFDS ILRATTMSFL LPNINLSTVF
     FTDTSFLNLC WEEKFKNELL SLQAPITNGL VTNESSVSLT DSVPDSIYDD ITSEHVDLTK
     RSVIKKCCRC GTFTRAGYPV DGQNTVVSTS IVTKRWTALY YRNCHCTGLL YEIKLK