MED16_MOUSE
ID MED16_MOUSE Reviewed; 828 AA.
AC Q6PGF3; E9QM81;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE AltName: Full=Mediator complex subunit 16;
DE AltName: Full=Thyroid hormone receptor-associated protein 5;
DE AltName: Full=Thyroid hormone receptor-associated protein complex 95 kDa component;
DE Short=Trap95;
DE AltName: Full=Vitamin D3 receptor-interacting protein complex 92 kDa component;
DE Short=DRIP92;
GN Name=Med16; Synonyms=Thrap5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000312|EMBL:AAH57056.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH57056.1};
RC TISSUE=Brain {ECO:0000312|EMBL:AAH57056.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC {ECO:0000305}.
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DR EMBL; AC151846; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC057056; AAH57056.1; -; mRNA.
DR CCDS; CCDS23996.2; -.
DR CCDS; CCDS48623.1; -.
DR PDB; 6W1S; EM; 4.02 A; K=1-828.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q6PGF3; -.
DR SMR; Q6PGF3; -.
DR BioGRID; 229709; 24.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR CORUM; Q6PGF3; -.
DR DIP; DIP-48408N; -.
DR IntAct; Q6PGF3; 7.
DR MINT; Q6PGF3; -.
DR STRING; 10090.ENSMUSP00000129375; -.
DR iPTMnet; Q6PGF3; -.
DR PhosphoSitePlus; Q6PGF3; -.
DR EPD; Q6PGF3; -.
DR MaxQB; Q6PGF3; -.
DR PaxDb; Q6PGF3; -.
DR PeptideAtlas; Q6PGF3; -.
DR PRIDE; Q6PGF3; -.
DR ProteomicsDB; 295860; -.
DR MGI; MGI:2158394; Med16.
DR eggNOG; ENOG502QQ3H; Eukaryota.
DR InParanoid; Q6PGF3; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR ChiTaRS; Med16; mouse.
DR PRO; PR:Q6PGF3; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q6PGF3; protein.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISS:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IC:MGI.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR021665; Mediator_Med16.
DR InterPro; IPR011041; Quinoprot_gluc/sorb_DH.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13224; PTHR13224; 1.
DR Pfam; PF11635; Med16; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50952; SSF50952; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..828
FT /note="Mediator of RNA polymerase II transcription subunit
FT 16"
FT /id="PRO_0000051293"
FT REPEAT 67..106
FT /note="WD 1"
FT REPEAT 198..240
FT /note="WD 2"
FT REPEAT 263..307
FT /note="WD 3"
FT REPEAT 621..662
FT /note="WD 4"
FT REPEAT 777..816
FT /note="WD 5"
FT CONFLICT 33
FT /note="I -> T (in Ref. 2; AAH57056)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="M -> L (in Ref. 2; AAH57056)"
FT /evidence="ECO:0000305"
FT CONFLICT 409
FT /note="P -> L (in Ref. 2; AAH57056)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 828 AA; 91786 MW; 6388AEF86575997D CRC64;
MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE
VHSVSSGHSE AITCLEWDQS GSRLLSADAD GQIKCWSMAD HLANSWESSV GSQVEGDPIV
ALSWLHNGVK LALHVEKSGA SSFGEKFSRV KFSPSLTLFG GKPMEGWIAV TVSGLVTVSL
LKPSGQVLTS TESLCRLRGR VALADIAFTG GGNIVVAAAD GSSASPVKFY KVCVSVVSEK
CRIDTEILPS LFMRCTTDPN RKDRFPAITH LKFLARDMSE QVLLCASSQT SSLVECWSLR
KEGLPVNNIF QQISPVVGDK QPMILKWRIL SATNDLDRVS AVALPKLPIS LTNTDLKVAS
DTQFYPGLGL ALAFQDGSVH MVHRLSLQTM AVFYSSAPRS LDEPALKRPR TTCPAVHFKA
MQLSWTSLAL VGIDNHGKLS MLRISPSLGH PLEPKLALQH LLFLLEYCMV TGYDWWDILL
HVQPGMVQSL VERLHEEYTR QKPALQQVLS TRILAMKASL CKLSPCTVAR VCDYHTKLFL
MAITSTLKSL LRPHFLNTPD KSPGDRLAEI CAKITDVDID KVMINLKTEE FVLDMNTLQA
LQQLLQWVGD FVLYLLVSLP NQGSPLRPGH SFLRDGTSLG MLRELMVVIR IWGLLKPSCL
PVYTATSDTQ DSMSLLFRLL TKLWICCRDE GAASEPDEGL VDECCLLPSQ LLVPNLDWLP
ASDGLVSRLQ PKQPLRLRFG RAPTLPSSTS TLQLDGLTRA PGQPKIDHLR RLHLGAYPTE
ECKACTRCGC VTMLKSPNKT TAVTQWEQRW IKNCLCGGLW RRVPLSCS
