ID   MED16_PICST             Reviewed;        1020 AA.
AC   A3LY88; A3LY89;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 3.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 16;
DE   AltName: Full=Mediator complex subunit 16;
GN   Name=SIN4; Synonyms=MED16; ORFNames=PICST_48926, PICST_61313;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABN67590.2; Type=Erroneous gene model prediction; Note=The predicted gene PICST_61313 has been split into 2 genes: PICST_61313 and PICST_48926.; Evidence={ECO:0000305};
CC       Sequence=ABN67591.2; Type=Erroneous gene model prediction; Note=The predicted gene PICST_61313 has been split into 2 genes: PICST_61313 and PICST_48926.; Evidence={ECO:0000305};
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DR   EMBL; CP000500; ABN67590.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP000500; ABN67591.2; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001385619.2; XM_001385582.1.
DR   RefSeq; XP_001385620.2; XM_001385583.1.
DR   AlphaFoldDB; A3LY88; -.
DR   STRING; 4924.XP_001385619.2; -.
DR   PRIDE; A3LY88; -.
DR   EnsemblFungi; ABN67590; ABN67590; PICST_61313.
DR   EnsemblFungi; ABN67591; ABN67591; PICST_48926.
DR   GeneID; 4840400; -.
DR   GeneID; 4840401; -.
DR   KEGG; pic:PICST_48926; -.
DR   KEGG; pic:PICST_61313; -.
DR   eggNOG; ENOG502QWAC; Eukaryota.
DR   HOGENOM; CLU_013428_0_0_1; -.
DR   InParanoid; A3LY88; -.
DR   OrthoDB; 209987at2759; -.
DR   Proteomes; UP000002258; Chromosome 6.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   InterPro; IPR021665; Mediator_Med16.
DR   PANTHER; PTHR13224; PTHR13224; 1.
DR   Pfam; PF11635; Med16; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1020
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   16"
FT                   /id="PRO_0000307631"
SQ   SEQUENCE   1020 AA;  113303 MW;  1B0D1D5DBCD0A318 CRC64;
     MARTASGATR PSDLISWSRN GFIAYACPVQ HSKDNLLLTY LENVNGNSWK LAEPQSISVK
     LENNFLSELA LVEWSYLSTD LAISDVYGNF YILLAGIGLL EPGSEKSTVN SDSSGAASTA
     AASQNSPSYE LTSYNHMEMI YRDIINQDPA LPVNPGAAIV SFSWLNIEKV QIINKPAALV
     TLENQNSSNS PFVYTYGVNQ FQPHGVTHPI STKQACVALR QNGQFTLYFQ GEHKVEYHKT
     SVALSDDIVI ITKASIGFTN DKQIVVTAYD SVTRNISTYS VTVDWGFLVE SAKRQKVDPH
     YHTPKEEHKT PKLNVTRISA SIPVPTFLAA AEEEGMDMDK MDVDADSEDG NQPSYQIGSL
     ASIDIISAGA EKDSSLDILI SYTFEDGSGI ASSTIYRYSL SEESELISSA FGELGVRKNV
     TAPANSPTVQ TVTLQDKLIR SGTIQSITTA LSDYFILLYY LDGHVDVVDR NSMKIVNNSD
     DTNLPPKTIS SIFDVGFNFP KIDNSSNLIM AVSPNLTSVA YADFKGGSTR LNLKVFEKEK
     YLGISPKELF ATSVGFAFRH AYACYTNTCS DDLVALIQTE IVRLQISLQK TITDKPHNIE
     MIIKKFVESI VSESHKAINF QLDAFNKESV DKLLSNPPLQ KLLSLQLVLG ELQDKNSIVS
     DIAWIVLNLR STSFGIMFSL SSIYRQISKK KPTEDSLQDS ITRGECIMSL VGNVKWLIDL
     MVYINQELLQ LSYSKQDSSN SKLTIKNSIA LPIILSKVPR LFLMYALSSI GRTHEILKKL
     HKDLSEASKL FTPMKESLNR YFTICNNSPL TLSLFENFLR ECDALITKEQ SIRLAGKEKG
     YSLKVEQKLV CQGELTEDME QIGKILIDRH AVNINRDMKV SDLYFYDVDW LDIGVNKRSA
     RTSKGLSETV IYPSSQLKPK MIPRLQYSDK ECIDSLRKII ISVDMNQITG KTDTSRNGTT
     NHKAAIANDK IAKLRKCTRC RSVSLVADPL VFDAPSTIGL WTMVFQRTCI CGNAWVNCVS