MED17_MOUSE
ID MED17_MOUSE Reviewed; 649 AA.
AC Q8VCD5; Q8BYS6; Q99KQ1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 17;
DE AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 6;
DE Short=CRSP complex subunit 6;
DE AltName: Full=Mediator complex subunit 17;
DE AltName: Full=Thyroid hormone receptor-associated protein complex 80 kDa component;
GN Name=Med17; Synonyms=Crsp6, Trap80;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Hypothalamus, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PPARG.
RX PubMed=12037571; DOI=10.1038/417563a;
RA Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M.,
RA Roeder R.G.;
RT "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated
RT adipogenesis.";
RL Nature 417:563-567(2002).
RN [4]
RP INTERACTION WITH GATA1, AND ASSOCIATION WITH PROMOTER REGIONS.
RX PubMed=17132730; DOI=10.1073/pnas.0604494103;
RA Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X.,
RA Guyot B., Roeder R.G., Borggrefe T.;
RT "The mediator complex functions as a coactivator for GATA-1 in
RT erythropoiesis via subunit Med1/TRAP220.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:18504-18509(2006).
RN [5]
RP ERRATUM OF PUBMED:17132730.
RA Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X.,
RA Guyot B., Roeder R.G., Borggrefe T.;
RL Proc. Natl. Acad. Sci. U.S.A. 104:1442-1442(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2 (By
CC similarity). Interacts with GATA1 and PPARG. {ECO:0000250,
CC ECO:0000269|PubMed:12037571, ECO:0000269|PubMed:17132730}.
CC -!- INTERACTION:
CC Q8VCD5; Q04207: Rela; NbExp=4; IntAct=EBI-5744951, EBI-644400;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 17 family.
CC {ECO:0000305}.
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DR EMBL; AK033151; BAC28172.1; -; mRNA.
DR EMBL; AK038451; BAC30005.1; -; mRNA.
DR EMBL; AK049112; BAC33548.1; -; mRNA.
DR EMBL; AK151377; BAE30349.1; -; mRNA.
DR EMBL; BC004062; AAH04062.1; -; mRNA.
DR EMBL; BC020185; AAH20185.1; -; mRNA.
DR CCDS; CCDS22834.1; -.
DR RefSeq; NP_659182.1; NM_144933.1.
DR PDB; 6W1S; EM; 4.02 A; L=15-645.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q8VCD5; -.
DR SMR; Q8VCD5; -.
DR BioGRID; 231602; 5.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR CORUM; Q8VCD5; -.
DR DIP; DIP-48407N; -.
DR IntAct; Q8VCD5; 11.
DR MINT; Q8VCD5; -.
DR STRING; 10090.ENSMUSP00000034411; -.
DR iPTMnet; Q8VCD5; -.
DR PhosphoSitePlus; Q8VCD5; -.
DR EPD; Q8VCD5; -.
DR MaxQB; Q8VCD5; -.
DR PaxDb; Q8VCD5; -.
DR PeptideAtlas; Q8VCD5; -.
DR PRIDE; Q8VCD5; -.
DR ProteomicsDB; 295988; -.
DR DNASU; 234959; -.
DR Ensembl; ENSMUST00000034411; ENSMUSP00000034411; ENSMUSG00000031935.
DR GeneID; 234959; -.
DR KEGG; mmu:234959; -.
DR UCSC; uc009ofl.1; mouse.
DR CTD; 9440; -.
DR MGI; MGI:2182585; Med17.
DR VEuPathDB; HostDB:ENSMUSG00000031935; -.
DR eggNOG; KOG4512; Eukaryota.
DR GeneTree; ENSGT00390000011810; -.
DR HOGENOM; CLU_028003_1_0_1; -.
DR InParanoid; Q8VCD5; -.
DR OMA; CQIYQHQ; -.
DR OrthoDB; 976669at2759; -.
DR PhylomeDB; Q8VCD5; -.
DR TreeFam; TF323615; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 234959; 24 hits in 76 CRISPR screens.
DR ChiTaRS; Med17; mouse.
DR PRO; PR:Q8VCD5; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8VCD5; protein.
DR Bgee; ENSMUSG00000031935; Expressed in primary oocyte and 244 other tissues.
DR ExpressionAtlas; Q8VCD5; baseline and differential.
DR Genevisible; Q8VCD5; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005667; C:transcription regulator complex; ISO:MGI.
DR GO; GO:0000151; C:ubiquitin ligase complex; IEA:Ensembl.
DR GO; GO:0030374; F:nuclear receptor coactivator activity; ISO:MGI.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; ISO:MGI.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:MGI.
DR InterPro; IPR019313; Mediator_Med17.
DR PANTHER; PTHR13114; PTHR13114; 1.
DR Pfam; PF10156; Med17; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..649
FT /note="Mediator of RNA polymerase II transcription subunit
FT 17"
FT /id="PRO_0000304700"
FT REGION 51..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 550
FT /note="S -> G (in Ref. 1; BAC30005)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 649 AA; 72462 MW; 924D5C2D4150B4B5 CRC64;
MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS QGSGSEEEEA
AGPDGDAPDW GGAGADQDDE EGLVKFQPSL WPWDSVRNNL RSALTEMCVL YDVLSIVRDK
KFMTLDPVSQ DALPPKQSPQ TLQLISKKKS LAGAAQILLK GAERLTKSVA ENQENKLQRD
FNSELLRLRQ HWKLRKVGDK ILGDLSYRSA GSLFPHHGTF EVIKNTDIDL DKKIPEDYCP
LDVQIPSDLE GSAYIKVSIQ KQAPDIGDLG TVNLFKRPLP KSKPGSPHWQ TKLEAAQNVL
LCKEIFAQLS REAVQIKSQI PHIVVKNQII SQPFPSLQLS ISLCHSSDDK KSQKCAAEKP
GQEDHLYVLE HNLHLLIREF HKQTLSSIVM PHPASAPFGH KRMRLSGPQA FDKNEINSIQ
STEGLLEKII KQAKHIFLRS RTAATIDSLA SRIEDPQIQA HWSNINDVYE SSVKVLITSQ
GYEQICKSIQ LQLNIGVEQV RVVHRDGRVI MLSHQEQELQ DFLLSQMSQH QVHAVQQLAK
VMGWQVLSFS NHVGLGPIES IGNASAITVA SPSGDYAISV RNGPESGSKI MVQFPRNQCK
DLPKSDVLQD SKWSHLRGPF KEVQWNKMEG RNFVYKMELL MSALSPCLL
