MED18_ARATH
ID MED18_ARATH Reviewed; 219 AA.
AC Q9SJZ6;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 18 {ECO:0000303|PubMed:17560376};
GN Name=MED18 {ECO:0000303|PubMed:17560376};
GN Synonyms=MED18_1 {ECO:0000303|PubMed:22021418};
GN OrderedLocusNames=At2g22370 {ECO:0000312|Araport:AT2G22370};
GN ORFNames=F14M13.23 {ECO:0000312|EMBL:AAD22361.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH YY1; ABI4 AND SUF4, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24451981; DOI=10.1038/ncomms4064;
RA Lai Z., Schluttenhofer C.M., Bhide K., Shreve J., Thimmapuram J., Lee S.Y.,
RA Yun D.-J., Mengiste T.;
RT "MED18 interaction with distinct transcription factors regulates multiple
RT plant functions.";
RL Nat. Commun. 5:3064-3064(2014).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional pre-initiation complex with RNA polymerase II and the
CC general transcription factors (PubMed:22021418, PubMed:24451981).
CC Involved in the regulation of histone H3 lysine tri-methylation
CC (H3K36me3). Associates with the promoter, coding and terminator regions
CC of target genes suggesting its function in transcription initiation,
CC elongation and termination. Multifunctional protein which regulates
CC plant immunity, especially during necrotrophic fungal infection (e.g.
CC B.cinerea and A.brassicicola), flowering time and responses to hormones
CC (e.g. abscisic acid ABA and ethylene) through interactions with
CC distinct transcription factors (PubMed:24451981).
CC {ECO:0000269|PubMed:22021418, ECO:0000269|PubMed:24451981}.
CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376). Interacts
CC with YY1 to suppress disease susceptibility via the repression of genes
CC glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to
CC regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in
CC the presence of abscisic acid (ABA). Interacts with SUF4 to regulate
CC flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981).
CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:24451981}.
CC -!- INTERACTION:
CC Q9SJZ6; Q6NPF4: MED20A; NbExp=3; IntAct=EBI-1386265, EBI-1386270;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24451981}.
CC -!- DISRUPTION PHENOTYPE: Deregulated expression of glutaredoxins GRX480,
CC GRXS13 and thioredoxin TRX-h5 leading to enhanced susceptibility to
CC fungal infection (e.g. B.cinerea and A.brassicicola). Altered RNA
CC polymerase II occupancy and histone H3 lysine tri-methylation
CC (H3K36me3) of target genes. Insensitivity to abscisic acid (ABA) and to
CC the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC).
CC Delayed flowering. {ECO:0000269|PubMed:24451981}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 18 family.
CC {ECO:0000305}.
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DR EMBL; AC006592; AAD22361.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07298.1; -; Genomic_DNA.
DR EMBL; BT029378; ABK32192.1; -; mRNA.
DR PIR; H84611; H84611.
DR RefSeq; NP_565534.1; NM_127802.4.
DR AlphaFoldDB; Q9SJZ6; -.
DR SMR; Q9SJZ6; -.
DR BioGRID; 2122; 2.
DR IntAct; Q9SJZ6; 3.
DR STRING; 3702.AT2G22370.1; -.
DR PaxDb; Q9SJZ6; -.
DR PRIDE; Q9SJZ6; -.
DR ProteomicsDB; 239052; -.
DR EnsemblPlants; AT2G22370.1; AT2G22370.1; AT2G22370.
DR GeneID; 816769; -.
DR Gramene; AT2G22370.1; AT2G22370.1; AT2G22370.
DR KEGG; ath:AT2G22370; -.
DR Araport; AT2G22370; -.
DR TAIR; locus:2041243; AT2G22370.
DR eggNOG; KOG3264; Eukaryota.
DR HOGENOM; CLU_111349_0_0_1; -.
DR InParanoid; Q9SJZ6; -.
DR OMA; QAEPTWT; -.
DR OrthoDB; 1140930at2759; -.
DR PhylomeDB; Q9SJZ6; -.
DR PRO; PR:Q9SJZ6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJZ6; baseline and differential.
DR Genevisible; Q9SJZ6; AT.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048440; P:carpel development; IGI:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0035196; P:miRNA processing; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0048441; P:petal development; IGI:TAIR.
DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:UniProtKB.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IDA:UniProtKB.
DR GO; GO:2000142; P:regulation of DNA-templated transcription, initiation; IDA:UniProtKB.
DR GO; GO:0031554; P:regulation of DNA-templated transcription, termination; IDA:UniProtKB.
DR GO; GO:2001253; P:regulation of histone H3-K36 trimethylation; IMP:UniProtKB.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0010219; P:regulation of vernalization response; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB.
DR GO; GO:0048442; P:sepal development; IGI:TAIR.
DR GO; GO:0048833; P:specification of floral organ number; IMP:TAIR.
DR GO; GO:0048443; P:stamen development; IGI:TAIR.
DR GO; GO:0006369; P:termination of RNA polymerase II transcription; IBA:GO_Central.
DR InterPro; IPR019095; Mediator_Med18.
DR PANTHER; PTHR13321; PTHR13321; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Developmental protein;
KW Ethylene signaling pathway; Nucleus; Plant defense; Reference proteome;
KW Transcription; Transcription regulation; Transcription termination.
FT CHAIN 1..219
FT /note="Mediator of RNA polymerase II transcription subunit
FT 18"
FT /id="PRO_0000418352"
SQ SEQUENCE 219 AA; 23365 MW; FE53D1C081F36F55 CRC64;
MSMECVVQGI IETQHVEALE ILLQGLCGVQ RERLRVHELC LRSGPNLGVV SSEVRLLCDL
DQPEPTWTVK HVGGAMRGAG ADQISVLVRN MIESKVSKNA LRMFYALGYK LDHELLKVGF
AFHFQRTAHI SVSVSSVNKM PKVHAIDEAV PVTPGMQIVD VTAPATSENY SEVAAAVSSF
CEFLAPLVHL SKPSISTGVV PTAAAAAASL MSDGGGTTL
