MED18_DROME
ID MED18_DROME Reviewed; 217 AA.
AC Q9XZT1; C0PTW2; Q8MS08;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 18;
DE AltName: Full=Mediator complex subunit 18;
DE AltName: Full=dp28b;
GN Name=MED18; Synonyms=arm; ORFNames=CG14802;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION OF THE MEDIATOR COMPLEX, IDENTIFICATION IN A COMPLEX WITH CDK8;
RP MED4; MED6; MED14; MED17; MED20; MED21 AND MED31, AND DEVELOPMENTAL STAGE.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, FUNCTION OF THE MEDIATOR COMPLEX, AND INTERACTION WITH MED6 AND
RP MED17.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000269|PubMed:11259581,
CC ECO:0000269|PubMed:12021283}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22,
CC MED27, MED28, MED30 and MED31. {ECO:0000269|PubMed:11259581,
CC ECO:0000269|PubMed:12021283}.
CC -!- INTERACTION:
CC Q9XZT1; P91641: MED20; NbExp=4; IntAct=EBI-148856, EBI-175591;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expression decreases during larval stages then rises during mid-pupal
CC metamorphosis. {ECO:0000269|PubMed:11259581}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 18 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM51027.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF45675.3; -; Genomic_DNA.
DR EMBL; AL021106; CAB41707.1; -; Genomic_DNA.
DR EMBL; AY119167; AAM51027.1; ALT_SEQ; mRNA.
DR EMBL; BT071807; ACN52010.1; -; mRNA.
DR PIR; T12679; T12679.
DR RefSeq; NP_569948.3; NM_130592.4.
DR AlphaFoldDB; Q9XZT1; -.
DR SMR; Q9XZT1; -.
DR BioGRID; 57686; 43.
DR DIP; DIP-17703N; -.
DR IntAct; Q9XZT1; 1.
DR STRING; 7227.FBpp0070295; -.
DR PaxDb; Q9XZT1; -.
DR DNASU; 31140; -.
DR EnsemblMetazoa; FBtr0070308; FBpp0070295; FBgn0026873.
DR GeneID; 31140; -.
DR KEGG; dme:Dmel_CG14802; -.
DR CTD; 54797; -.
DR FlyBase; FBgn0026873; MED18.
DR VEuPathDB; VectorBase:FBgn0026873; -.
DR eggNOG; KOG3264; Eukaryota.
DR GeneTree; ENSGT00390000003312; -.
DR HOGENOM; CLU_084570_0_0_1; -.
DR InParanoid; Q9XZT1; -.
DR OMA; PTSPWHL; -.
DR PhylomeDB; Q9XZT1; -.
DR BioGRID-ORCS; 31140; 1 hit in 1 CRISPR screen.
DR ChiTaRS; arm; fly.
DR GenomeRNAi; 31140; -.
DR PRO; PR:Q9XZT1; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0026873; Expressed in secondary oocyte and 22 other tissues.
DR ExpressionAtlas; Q9XZT1; baseline and differential.
DR Genevisible; Q9XZT1; DM.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006369; P:termination of RNA polymerase II transcription; IBA:GO_Central.
DR InterPro; IPR019095; Mediator_Med18.
DR PANTHER; PTHR13321; PTHR13321; 1.
DR Pfam; PF09637; Med18; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..217
FT /note="Mediator of RNA polymerase II transcription subunit
FT 18"
FT /id="PRO_0000304749"
SQ SEQUENCE 217 AA; 24673 MW; CF0A9E0215A56560 CRC64;
MAIVSSARES LSHAMNNRFL PNLEYLLQGS ILDSAVEHLM HRLKGLCDNV DTSPEPFHDL
EVCMSLRQPN ANQPLLLRVR RALGRDAPFQ MRYLGNPEVD LRRPTLVRSC MDCACTNGIL
EFLTEMGFRL EFEYIAKGYM FRKGRMKITV SKLIKIVPGK QQDMANEPIS QSYIVELSVV
APTGQENVGE EMRVFAEQLK PLVQLEKIDY KRLGGMP
