MED19_DROME
ID MED19_DROME Reviewed; 337 AA.
AC Q9VVL6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 19;
DE AltName: Full=Mediator complex subunit 19;
GN Name=MED19; ORFNames=CG5546;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9VVL6; Q8SYU5: Dmel\CG10086; NbExp=3; IntAct=EBI-125442, EBI-116042;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 19 family.
CC {ECO:0000305}.
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DR EMBL; AE014296; AAF49295.1; -; Genomic_DNA.
DR EMBL; AY069647; AAL39792.1; -; mRNA.
DR RefSeq; NP_001287103.1; NM_001300174.1.
DR RefSeq; NP_649016.1; NM_140759.3.
DR AlphaFoldDB; Q9VVL6; -.
DR BioGRID; 65272; 32.
DR IntAct; Q9VVL6; 12.
DR STRING; 7227.FBpp0074926; -.
DR PaxDb; Q9VVL6; -.
DR PRIDE; Q9VVL6; -.
DR DNASU; 39987; -.
DR EnsemblMetazoa; FBtr0075160; FBpp0074926; FBgn0036761.
DR EnsemblMetazoa; FBtr0345815; FBpp0311801; FBgn0036761.
DR GeneID; 39987; -.
DR KEGG; dme:Dmel_CG5546; -.
DR CTD; 219541; -.
DR FlyBase; FBgn0036761; MED19.
DR VEuPathDB; VectorBase:FBgn0036761; -.
DR eggNOG; KOG4043; Eukaryota.
DR HOGENOM; CLU_829689_0_0_1; -.
DR InParanoid; Q9VVL6; -.
DR OMA; GNMMDSP; -.
DR OrthoDB; 1484317at2759; -.
DR PhylomeDB; Q9VVL6; -.
DR BioGRID-ORCS; 39987; 0 hits in 1 CRISPR screen.
DR ChiTaRS; MED19; fly.
DR GenomeRNAi; 39987; -.
DR PRO; PR:Q9VVL6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036761; Expressed in cleaving embryo and 22 other tissues.
DR ExpressionAtlas; Q9VVL6; baseline and differential.
DR Genevisible; Q9VVL6; DM.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0008134; F:transcription factor binding; IPI:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR InterPro; IPR019403; Mediator_Med19_met.
DR PANTHER; PTHR22536; PTHR22536; 1.
DR Pfam; PF10278; Med19; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..337
FT /note="Mediator of RNA polymerase II transcription subunit
FT 19"
FT /id="PRO_0000304773"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 155..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 189..223
FT /evidence="ECO:0000255"
FT COMPBIAS 158..172
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..219
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 337 AA; 35390 MW; 46407EBF573EC80D CRC64;
MMSNYGNMMD SPKSSPHGGG RSPVVARQDS SGTLKTTISL GKTPTIIHTG PFYSMKEPPA
KAELTGDKDL MTEYGLHHTL TKFKEKKFKE SLASFLQNIP GINDLITHPV ENSTLRSVIE
KPPIGGKELL PLTPVQLAGF RLHPGPLPEQ YRTTYVTPAR KHKNKHKKQK HKDGVTTGQE
STLLDSAGLE TYEKKHKKQK RHEDDKERKK RKKEKKRKKK NQSPEPGVGL LPGGASLGGA
AGVMGATSLG SLGGGPGGPG LSSLSASMGP GVVGNMNSFS SSMQMQQQQQ MPMQQQQMSS
GGLLGSVLGT SGGPGGGGGG GGGGGGGVGG SLLMSQF
