MED19_SCHPO
ID MED19_SCHPO Reviewed; 138 AA.
AC Q9Y7N2;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 19;
DE AltName: Full=Mediator complex subunit 19;
DE AltName: Full=RNA polymerase II mediator complex protein rox3;
GN Name=med19; Synonyms=rox3; ORFNames=SPCC1450.05c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:11572939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 19 family.
CC {ECO:0000305}.
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DR EMBL; CU329672; CAB40172.1; -; Genomic_DNA.
DR PIR; T40987; T40987.
DR RefSeq; NP_588304.1; NM_001023294.2.
DR PDB; 5N9J; X-ray; 3.40 A; C=1-138.
DR PDBsum; 5N9J; -.
DR AlphaFoldDB; Q9Y7N2; -.
DR SMR; Q9Y7N2; -.
DR BioGRID; 275819; 72.
DR IntAct; Q9Y7N2; 1.
DR STRING; 4896.SPCC1450.05c.1; -.
DR iPTMnet; Q9Y7N2; -.
DR PaxDb; Q9Y7N2; -.
DR PRIDE; Q9Y7N2; -.
DR EnsemblFungi; SPCC1450.05c.1; SPCC1450.05c.1:pep; SPCC1450.05c.
DR GeneID; 2539249; -.
DR KEGG; spo:SPCC1450.05c; -.
DR PomBase; SPCC1450.05c; -.
DR VEuPathDB; FungiDB:SPCC1450.05c; -.
DR eggNOG; ENOG502R765; Eukaryota.
DR HOGENOM; CLU_1856455_0_0_1; -.
DR InParanoid; Q9Y7N2; -.
DR OMA; RSYKAYI; -.
DR PRO; PR:Q9Y7N2; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; IC:PomBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR013942; Mediator_Med19_fun.
DR PANTHER; PTHR28270; PTHR28270; 1.
DR Pfam; PF08633; Rox3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..138
FT /note="Mediator of RNA polymerase II transcription subunit
FT 19"
FT /id="PRO_0000096369"
FT TURN 8..11
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 26..29
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 33..39
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 56..59
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 73..79
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 90..97
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 109..112
FT /evidence="ECO:0007829|PDB:5N9J"
SQ SEQUENCE 138 AA; 15928 MW; 32D1261667B27D52 CRC64;
MAQAPEYHYV GSVDYQPTRP SAHQNLIELY GLTELAKKVG RVDEFGNKRK MRRSYKAYIQ
DLPGYNEILR DNTIKQWLTN PIREEVPIDI EFLHHVFSVE PGIIPGFNPK VFGLEDDVVM
GNVSRDSSQP RSPSRRKK
