MED1_CAEEL
ID MED1_CAEEL Reviewed; 1475 AA.
AC Q9N4G4; Q95Y43; Q9GPV1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 1.1;
DE AltName: Full=Mediator complex subunit 1.1;
DE AltName: Full=Suppressor of pal-1 protein 3;
GN Name=sop-3; Synonyms=mdt-1.1; ORFNames=Y71F9B.10;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RX PubMed=11171401; DOI=10.1242/dev.128.5.767;
RA Zhang H., Emmons S.W.;
RT "The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox
RT gene expression.";
RL Development 128:767-777(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9N4G4; Q8MPT2: T04C9.1; NbExp=6; IntAct=EBI-2315681, EBI-2315635;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q9N4G4-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q9N4G4-2; Sequence=VSP_027908, VSP_027909;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF308860; AAG45416.1; -; mRNA.
DR EMBL; FO081276; CCD70417.1; -; Genomic_DNA.
DR EMBL; FO081276; CCD70418.1; -; Genomic_DNA.
DR RefSeq; NP_001021811.1; NM_001026640.3.
DR RefSeq; NP_740821.1; NM_170837.3. [Q9N4G4-1]
DR AlphaFoldDB; Q9N4G4; -.
DR BioGRID; 37321; 3.
DR IntAct; Q9N4G4; 2.
DR STRING; 6239.Y71F9B.10a; -.
DR iPTMnet; Q9N4G4; -.
DR EPD; Q9N4G4; -.
DR PaxDb; Q9N4G4; -.
DR PeptideAtlas; Q9N4G4; -.
DR PRIDE; Q9N4G4; -.
DR EnsemblMetazoa; Y71F9B.10a.1; Y71F9B.10a.1; WBGene00004946. [Q9N4G4-1]
DR EnsemblMetazoa; Y71F9B.10b.1; Y71F9B.10b.1; WBGene00004946.
DR GeneID; 171840; -.
DR UCSC; Y71F9B.10a.1; c. elegans. [Q9N4G4-1]
DR CTD; 171840; -.
DR WormBase; Y71F9B.10a; CE28808; WBGene00004946; sop-3. [Q9N4G4-1]
DR WormBase; Y71F9B.10b; CE29912; WBGene00004946; sop-3. [Q9N4G4-2]
DR eggNOG; ENOG502QPZ7; Eukaryota.
DR GeneTree; ENSGT00970000196378; -.
DR InParanoid; Q9N4G4; -.
DR SignaLink; Q9N4G4; -.
DR PRO; PR:Q9N4G4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00004946; Expressed in embryo and 4 other tissues.
DR ExpressionAtlas; Q9N4G4; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1475
FT /note="Mediator of RNA polymerase II transcription subunit
FT 1.1"
FT /id="PRO_0000302025"
FT REGION 579..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 645..894
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 908..1475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1098..1135
FT /evidence="ECO:0000255"
FT COMPBIAS 580..600
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 690..714
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 754..804
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 863..879
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 913..943
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 968..990
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1027..1052
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1053..1178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1198..1220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1221..1266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1279..1308
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1317..1332
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1389..1403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1419..1440
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1441..1475
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 536..544
FT /note="VTVLEVGPA -> ELYALTTSK (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_027908"
FT VAR_SEQ 545..1475
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_027909"
SQ SEQUENCE 1475 AA; 164547 MW; C0A80E70DE9CC98B CRC64;
MIIEERKNHE LNLESIDRDL RMENVRQSAE KLGWEHFARS VRRNLLEKRQ TLDARTRLDV
LGSLVFMKEH LPIDNDATIA RKVQQLSEGL GEHCVFSTQN SGFSIRNPDV TIDIGVAEDQ
ISTCKIGYFG QPLTDAPGAL ALMKNGEFSK LRDSITAILS SLPKEITLSE KNACKEAFRA
LDQLLIRDAA DSSFTAINTG KYGFYAPRNE LRSGRIYYVA EPVLIRLAEK EGKTKADASY
LDLLPYIEIS FFKHDKPSKL PVFTQTGEWS TFNDANVCIC VRFNQGFLLS QQTIRKLSHI
VPKSPLVRNY VNFYRYMTGR VGVKSNLKLL TQFPDEGDIQ QQYQVDSKML TKEDDVVAME
MYLSDFRDLS KLIETLRSEW KHASLWESIL AICESSKGEQ KEVNAVDMQL VLRRTEFFLQ
FDTDYGSMTM QIFEKSPSNY VVKTTSQITG ESPSLDFDKM LTEKLNSTWS IPATLTFAIT
DLKCRINSVL NCVISFDSSS SSDKQHQNYW SISNNNTQLM QSAAQKSAAK CTKKKVTVLE
VGPAIEPTQL VYTRENDEEF QGSLLSSFGE VDDYYVKTST IGRTRPQPRP KREPNQLTSM
DMMSAELDKS RHTMEGSMRD AQTSSFLDAA RVRMKQSIGA AHAVGLASHQ PPASPVAAAA
AAPGGPMRHN SYPSGFEPPP SVAPYDFPAD PSKKQRKQRA KKQPGEEPPA GRGKGGKKGR
GAGAVGAAAA GGRKSAGGAG ENPFGMDPMR PPMLQRSSSE QHNPNPHQMS QYQMQQYQQN
QQFRMHQMQQ QQQQQFQMQS PKNPLVPAYT DEDSDEECDP PPPPKPQVST SSRMSSVPSH
PPPPPQQLGN PMVGYPGMPL QSPNHLPLTP SPLSAPPKPF SPEQQHHFGM KMRETSYWKE
LERLETKPDV EKLKQQLSSS SSSSQAEASD PPSTSTEAQD PPKPSSSSAP PPPKKKLGLE
AAISKIRGQQ EQALQKQLQQ QESVESELAT PHPGSSGAPP LAPHQVNRAR NLNDIFDDEA
DDSSPTADIK PSLQALQKPT DTSSQSTSSE PPPKKEPADE QPEREKEKLI LKIPKMLKPV
VDDRREDRKE RDRDRDRDRD RDEDREKVRD KEDKAQREKD KKEKERERRR QRDRDRTEQK
KSDREKEPSK KRKLEKKDEK EKDRREPERK KGKSDGKEYS KASTTSLIPT LSLKNFRIPK
KDTVEEEKKD VKEEAPGPSS STESKKEPPS AAPITRKEST TAPVAPAVQQ QQQRKESFTA
LPSLPEQQQH HREPLLKKKP PLQPPPPPQM TGPPIGIYPG PPAPGNLPGS SRPSGNRKQP
LPPPPPMIRG PPPDQMYRDR SGGGGGSMRG GFPPASHYHG GTSGVNKQVA SYAQGLPPGM
GPPVVKPHGN NYQASQWVRP PTHRDSSHSY HGMPSLGPPQ LQREPPAPPP PQMIPLPKDP
PVLREHPREH RAHRGGADDD GPDSPEEGTL RIDDE